Australian water security and Asian food security: complexity and macroeconomics of sustainability

The thesis focuses on the macroeconomics of sustainable development and the extension to energy, water and food security, using a system dynamics approach, i.e. the methods of differential equations systems with initial values. The work is divided into three related parts that build a narrative concerning the interaction between economics, policy, natural resources and society. First, after reviewing the concepts of complexity in environmental security, a simple system comprising three coupled differential equations is used to explain the effects of macroeconomic business cycles on the exploitation of ecological resources, and from this is inferred an implied importance of averting business cycles. The concept of entropy production is used to represent the exploitation of ecological resources. The second part establishes a system methodology inspired by Post Keynesian economics to develop the Murray-Darling Basin Economy Simulation Model that links food production/water users and food consumers at the micro scale, to the macroeconomic system dynamics. The goal of this study is to integrate and analyze the ecological-economic system in the Murray-Darling basin. The concepts of entropy production, useful work and income distribution are used as a bridge between the micro and macro subsystems. The system parameters are estimated using an ecological-economic data set for the Murray-Darling basin and for Australia (where data of the Basin are unavailable) from 1978-2005, and the model is validated using data from 2006-2012. The results reveal important structural linkages between the two subsystems and are used to predict the consequences of business cycles and government intervention for the coordination of growth and sustainability. The third, and final, part presents the development of an ``Asian Food Security Risk Engine'' that predicts the threat of civil unrest from food insecurity in Asian developing countries. A basal characteristics index for each developing country in Asia is defined and evaluated. Based on these measures, and introducing the concept of flow of anger, we use a differential equation system to integrate the threat of food security, the trigger for food riots, and food policy. The system parameters are estimated using a data set tracking indexes for threat, trigger and policy for Asian developing countries from 2006-2008, and the model is validated using data from 2009-2012. The results show the possible alternative approaches to simulating threat severity from food insecurity and are used to predict the threat of social unrest due to food security for a given country one month ahead

Hereditary familial vestibular degenerative diseases.

Item does not contain fulltextIdentification of genes involved in hereditary vestibular disease is growing at a remarkable pace. Mutant mouse technology can be an important tool for understanding the biological mechanism of human vestibular diseases

Parameters influencing the introduction of plasmid DNA into cells by the use of synthetic amphiphiles as a carrier system

Parameters that affect cellular transfection as accomplished by introducing DNA via carriers composed of cationic synthetic amphiphiles, have been investigated with the aim to obtain insight into the mechanism of DNA translocation. Such insight may be exploited in optimizing carrier properties of synthetic amphiphiles for molecules other than nucleic acids. In the present work, the interaction of vesicles composed of the cationic amphiphile dioleyloxy-propyl-trimethylammonium chloride (DOTMA) with cultured cells was examined. The results show that optimal transfection is dependent on the concentration of lipid, which determines the efficiency of vesicle interaction with the target cell membrane. as well as the toxicity of the amphiphiles towards the cell. A low lipid/DNA ratio prevents the complex from interacting with the cell surface, whereas at a relatively high amphiphile concentration the complex becomes toxic. Translocation efficiency is independent of the initial vesicle size but is affected by the size of the DNA. An incubation time of the DNA/amphiphile complex and cells of approx. 2-4 h is required for obtaining efficient transfection. In conjunction with observations on DNA/amphiphile complex-induced hemolysuis of erythrocytes. a mechanism of DNA-entry is proposed which involves translocation of the nucleic acids through pores acrosh the membranes rather than delicery via fusion or endocytosis. Dioleoylphosphatidylethanolamine, a phospholipid frequently used in a mixture with DOTMA ('lipofectin') strongly facilitates this pore formation. Translocation of the DNA is effectively prevented when the cell, are pretreated with Ca2+ or pronase. These observations suggest that Ca2+-sensitive cell surface proteins play a role in amphiphile-mediated DNA translocation.