一抗体免疫法によるPorphyromonas. gingivalis SODの定量(プレリミナリーレポート)

Superoxide dismutase (SOD) as a free radical scavenger in Porphyromonas gingivalis is well documented. The aim of this work was to develop an enzyme-linked immunosorbent assay using recombinant P. gingivalis SOD as an antigen. The sandwich complex was detected using a secondary antibody conjugated to β-D-galactosidase. Under optimum conditions, the sensitivity and the limit of detection were determined from 25pg to 500pg. In future, the application will be extended to the expression of SOD from P. gingivalis under various growing conditions

Aberrant TRPV1 Expression in Heat Hyperalgesia Associated with Trigeminal Neuropathic Pain

<p>Trigeminal neuropathic pain is a facial pain syndrome associated with trigeminal nerve injury. However, the mechanism of trigeminal neuropathic pain is poorly understood. This study aimed to determine the role of transient receptor potential vanilloid 1 (TRPV1) in heat hyperalgesia in a trigeminal neuropathic pain model. We evaluated nociceptive responses to mechanical and heat stimuli using a partial infraorbital nerve ligation (pIONL) model. Withdrawal responses to mechanical and heat stimuli to vibrissal pads (VP) were assessed using von Frey filaments and a thermal stimulator equipped with a heat probe, respectively. Changes in withdrawal responses were measured after subcutaneous injection of the TRP channel antagonist capsazepine. In addition, the expression of TRPV1 in the trigeminal ganglia was examined. Mechanical allodynia and heat hyperalgesia were observed in VP by pIONL. Capsazepine suppressed heat hyperalgesia but not mechanical allodynia. The number of TRPV1-positive neurons in the trigeminal ganglia was significantly increased in the large-diameter-cell group. These results suggest that TRPV1 plays an important role in the heat hyperalgesia observed in the pIONL model.</p