Antimicrobial activity against pathogenic bacteria, antioxidant and cytotoxic activity of cosmos caudatus

Cosmos caudatus is a traditional medicinal herb commonly found in Southeast Asia. This herb has been reported as a potential antioxidant and antimicrobial agent as it contains a variety of bioactive compounds.However, its cytotoxic effects on cancer still lack empirical evidence. Thus, this study aimed to investigate antimicrobial and antioxidant potentials of Cosmos caudatus as well as its cytotoxic effects on cancer cell lines. Ethanol and water extracts of Cosmos caudatus were tested for its antimicrobial activities against pathogenic bacteria, Staphylococcus aureus, Salmonella sp., Pseudomonas aeruginosa and Escherichia coli using agar well diffusion method. Antioxidant properties of the extracts were evaluated by 2,2-diphenyl-1-picryl-hydrazyl radical scavenging assay while cytotoxic activity of the extracts on colorectal carcinoma cell line, HCT 116 was determined by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The findings indicated that conventional Soxhlet extraction with water as solvent extracted more efficiently than ethanolic maceration and ultrasonic assisted extraction. In antioxidants assay, both ethanolic and water extracts obtained through ultrasonic extraction exhibited the highest DPPH free radical scavenging capacity compared to maceration and Soxhlet extraction method. As for antimicrobial assay, both water and ethanol extracts showed no inhibitory action against the tested pathogenic bacteria. For anticancer activity, the F2, F7 and F8 fractions showed potent dose dependent cytotoxicity on HCT 116 cells with IC50 value of 15.53 ± 0.4, 32.72 ± 0.3 and 34.16 ± 1.4 μg/mL, respectively

Low Temperature Geomicrobiology Follows Host Rock Composition Along a Geochemical Gradient in Lau Basin

The East Lau Spreading Center (ELSC) and Valu Fa Ridge (VFR) comprise a ridge segment in the southwest Pacific Ocean where rapid transitions in the underlying mantle chemistry manifest themselves as gradients in seafloor rock geochemistry. We studied the geology and microbial diversity of three silicate rock samples and three inactive sulfide chimney samples collected, from north to south, at the vent fields Kilo Moana, ABE, Tui Malila, and Mariner. This is the first study of microbial populations on basaltic andesite, which was sampled at Mariner vent field. Silicate rock geochemistry exhibits clear latitudinal trends that are mirrored by changes in bacterial community composition. α-proteobacteria, ε-proteobacteria, and Bacteroidetes are most common on a silicate collected from Kilo Moana and their proportions decrease linearly on silicates collected further south. Conversely, a silicate from Mariner vent field hosts high proportions of a unique lineage of Chloroflexi unrelated (<90% sequence similarity) to previously recovered environmental clones or isolates, which decrease at ABE and are absent at Kilo Moana. The exteriors of inactive sulfide structures are dominated by lineages of sulfur oxidizing α-proteobacteria, γ-proteobacteria, and ε-proteobacteria, while the interior of one chimney is dominated by putative sulfur-reducing δ-proteobacteria. A comparison of bacterial communities on inactive sulfides from this and previous studies reveals the presence of a clade of uncultured Bacteroidetes exclusive to sulfidic environments, and a high degree of heterogeneity in bacterial community composition from one sulfide structure to another. In light of the heterogeneous nature of bacterial communities observed here and in previous studies of both active and inactive hydrothermal sulfide structures, the presence of numerous niches may be detected on these structures in the future by finer scale sampling and analysis.Organismic and Evolutionary Biolog

Measurement of the eta-Meson Mass using psi(2S) --> eta J/psi

We measure the mass of the eta meson using psi(2S) --> eta J/psi events acquired with the CLEO-c detector operating at the CESR e+e- collider. Using the four decay modes eta --> gamma gamma, 3pi0, pi+pi-pi0, and pi+pi-gamma, we find M(eta)=547.785 +- 0.017 +- 0.057 MeV, in which the first uncertainty is statistical and the second systematic. This result has an uncertainty comparable to the two most precise previous measurements and is consistent with that of NA48, but is inconsistent at the level of 6.5sigma with the much smaller mass obtained by GEM.Comment: 10 pages postscript,also available through http://www.lns.cornell.edu/public/CLNS/2007/, Submitted to PR

Suppressed Decays of D_s^+ Mesons to Two Pseudoscalar Mesons

Using data collected near the Ds*+ Ds- peak production energy Ecm = 4170 MeV by the CLEO-c detector, we study the decays of Ds+ mesons to two pseudoscalar mesons. We report on searches for the singly-Cabibbo-suppressed Ds+ decay modes K+ eta, K+ eta', pi+ K0S, K+ pi0, and the isospin-forbidden decay mode Ds+ to pi+ pi0. We normalize with respect to the Cabibbo-favored Ds+ modes pi+ eta, pi+ eta', and K+ K0S, and obtain ratios of branching fractions: Ds+ to K+ eta / Ds+ to pi+ eta = (8.9 +- 1.5 +- 0.4)%, Ds+ to K+ eta' / Ds+ to pi+ eta' = (4.2 +- 1.3 +- 0.3)%, Ds+ to pi+ K0S / Ds+ to K+ K0S = (8.2 +- 0.9 +- 0.2)%, Ds+ to K+ pi0 / Ds+ to K+ K0S = (5.0 +- 1.2 +- 0.6)%, and Ds+ to pi+ pi0 / Ds+ to K+ K0S < 4.1% at 90% CL, where the uncertainties are statistical and systematic, respectively.Comment: 9 pages postscript,also available through http://www.lns.cornell.edu/public/CLNS/2007/, Submitted to PR

A Study of Exclusive Charmless Semileptonic B Decays and Extraction of |V_{ub}| at CLEO

We have studied semileptonic B decay to the exclusive charmless states pi, rho/omega, eta and eta' using the full 15.5 fb^-1 CLEO Upsilon(4S) sample, with measurements performed in subregions of phase space to minimize dependence on a priori knowledge of the form factors involved. We find total branching fractions B(B^0 -> pi^-l^+nu) = (1.37 +- 0.15_stat +- 0.11_sys) x 10^-4 and B(B^0 -> rho^- l^+ nu) = (2.93 +- 0.37_stat +- 0.37_sys) x 10^-4. We find evidence for B^+ -> eta' l^+ nu, with B(B^+ -> eta' l^+ nu) = (2.66 +- 0.80_stat +- 0.56_sys) x 10^-4 and 1.20 x 10^-4 eta' l^+ nu) < 4.46 x 10^-4 (90% CL). We also limit B(B^+ -> eta l^+ nu) < 1.01 x 10^-4 (90% CL). By combining our B -> pi l nu information with unquenched lattice calculations, we find |V_ub| = (3.6 +- 0.4 +- 0.2 +0.6 -0.4) x 10^-3, where the errors are statistical, experimental systematic, and theoretical systematic, respectively.Comment: 35 pages, 15 figures; revise

chi_cJ Decays to h^+h^-h^0

Using a sample of 3x10^6 psi(2S) decays recorded by the CLEO detector, we study three body decays of the chi_{c0}, chi_{c1}, and chi_{c2} produced in radiative decays of the psi(2S). We consider the decay modes pi+ pi- eta, K+ K- eta, p pbar eta, pi+ pi- etaprime, K+ K- pi0, p pbar pi0, pi+ K- K0S, and K+ pbar Lambda measuring branching fractions or placing upper limits. For chi_{c1} to pi+ pi- eta, K+ K- pi0, and pi+ K- K0S our observed samples are large enough to study the substructure in a Dalitz plot analysis. The results presented in this document are preliminary.Comment: 22 pages postscript,also available through http://www.lepp.cornell.edu/public/CONF/2006, Contributed to ICHEP0

Measurement of the Decay Constant fDS+f_D{_S^+} using $D_S^+ --> ell^+ nu

We measure the decay constant fDs using the Ds -> l+ nu channel, where the l+ designates either a mu+ or a tau+, when the tau+ -> pi+ nu. Using both measurements we find fDs = 274 +-13 +- 7 MeV. Combining with our previous determination of fD+, we compute the ratio fDs/fD+ = 1.23 +- 0.11 +- 0.04. We compare with theoretical estimates.Comment: 6 pages postscript,also available through http://www.lns.cornell.edu/public/CLNS/2007

Measurement of Interfering K^*+K^- and K^*-K^+ Amplitudes in the Decay D^0 --> K^+K^-pi^0

We have studied the Cabibbo-suppressed decay mode D^0 into K^+ K^- pi^0 using a Dalitz plot technique and find the strong phase difference delta_D [defined as delta_(K*^- K^+) - delta_(K*^+ K^-)] = 332 degrees +- 8 degrees +- 11 degrees and relative amplitude r_D [defined as a_(K*^- K^+) / a_(K*^+ K^-)] = 0.52 +- 0.05 +- 0.04. This measurement indicates significant destructive interference between D^0 into K^+ (K^- pi^0)_K*^- and D^0 into K^- (K^+ pi^0)_K*^+ in the Dalitz plot region where these two modes overlap. This analysis uses 9.0 fb^(-1) of data collected at s^(1/2) of approximately 10.58 GeV with the CLEO III detector.Comment: 10 pages postscript,also available through http://www.lns.cornell.edu/public/CLNS/2006/, Submitted to Phys. Rev. D (Rapid Communications

A Precision Determination of the D^0 Mass

A precision measurement of the D^0 meson mass has been made using ~281 pb-1 of e+e- annihilation data taken with the CLEO-c detector at the psi(3770) resonance. The exclusive decay D^0 -> (Ks phi) has been used to obtain M(D^0)=1864.847+-0.150(stat)+-0.095(syst) MeV. This corresponds to M(D^0 D^*0)=3871.81+-0.36 MeV, and leads to a well-constrained determination of the binding energy of the proposed (D^0 D^*0) molecule X(3872), E_b=0.6+-0.6 MeV.Comment: 8 pages postscript,also available through http://www.lns.cornell.edu/public/CLNS/2006/, Submitted to PR

Measurement of Absolute Hadronic Branching Fractions of D Mesons and e^+ e^- --> D D-bar Cross Sections at the psi(3770)

Using 281 /pb of e^+ e^- collisions recorded at the psi(3770) resonance with the CLEO-c detector at CESR, we determine absolute hadronic branching fractions of charged and neutral D mesons using a double tag technique. Among measurements for three D^0 and six D^+ modes, we obtain reference branching fractions B(D^0 --> K^-pi^+) = (3.891 +- 0.035 +- 0.059 +- 0.035)% and B(D^+ --> K^-pi^+pi^+) = (9.14 +- 0.10 +- 0.16 +- 0.07)%, where the first uncertainty is statistical, the second is all systematic errors other than final state radiation (FSR), and the third is the systematic uncertainty due to FSR. We include FSR in these branching fractions by allowing for additional unobserved photons in the final state. Using an independent determination of the integrated luminosity, we also extract the cross sections sigma(e+e- --> D^0 D^0-bar) = (3.66+- 0.03 +- 0.06) nb and sigma(e+e- --> D^+ D^-) = (2.91+- 0.03 +- 0.05) nb at a center of mass energy, E_cm = 3774 +- 1 MeV.Comment: 47 pages, postscript also available through this http://www.lns.cornell.edu/public/CLNS/2007/, to be published in PRD, updated branching fractions using B(KS0 --> pi+ pi-) from PDG 2007, and updated text in response to the PRD reviewe