Oxidative effects of extremely low frequency magnetic field and radio frequency radiation on testes tissues of diabetic and healthy rats

WOS: 000403031700006PubMed ID: 28516790With the development of technology, people are increasingly under the exposure of electromagnetic fields. Individuals with chronic diseases such as diabetes are now long-term exposed to Radio Frequency RF radiation and extremely low frequency (ELF) magnetic fields (MFs). The purpose of this present study is to investigate oxidative effects and antioxidant parameters of ELF MFs and RF radiation on testis tissue in diabetic and healthy rats. Wistar male rats were divided into 10 groups. Intraperitoneal single dose STZ (65 mg/kg) dissolved in citrate buffer (0.1M (pH 4.5)) was injected to diabetes groups. ELF MFs and RF radiation were used as an electromagnetic exposure for 20 min/day, 5 days/week for one month. Testis tissue oxidant malondialdehyde (MDA), and antioxidants glutathione (GSH), and total nitric oxide (NOx) levels were determined. The results of ANOVA and Mann Whitney tests were compared; p < 0.05 was considered significant. ELF and RF radiation resulted in an increase in testicular tissue MDA and NOx levels (p < 0.05), and caused a decrease in GSH levels (p < 0.05) in both healthy and diabetic rats, yet more distinctively in diabetic rats. The most pronounced effect was recorded in D-RF + ELF group (p < 0.005). Both radiation practices increased the oxidative stress in testis tissue while causing a decrease in antioxidant level which was more distinctive in diabetic rats (Tab. 1, Fig. 3, Ref. 30). Text in PDF www.elis.sk.Gazi University Research FoundationGazi University [31/2002-07, 01/2011-18]The authors thank the staff of the Gazi University Laboratory Animals and Experimental Researches Center. Electromagnetic field measurement devices used in this study were supplied by the grant from Gazi University Research Foundation, No. 31/2002-07. This study was supported by the grant from Gazi University Research Foundation, No. 01/2011-18. All animal rights were observed

Mitochondrial hyperpolarization and cytochrome-c release in microwave-exposed MCF-7 cells

In animals, the impact of ROS production by mitochondria on cell physiology, death, disease and ageing is well recognised. In photosynthetic organisms such as higher plants, however, the chloroplast and peroxisomes are the major sources of ROS during normal metabolism and the importance of mitochondria in oxidative stress and redox signalling is less well established. To address this, the in vivo oxidation state of a mitochondrially-targeted redox-sensitive GFP (mt-roGFP2) was investigated in Arabidopsis leaves. Classical ROS-generating inhibitors of mitochondrial electron transport (rotenone, antimycin A and SHAM) had no effect on mt-ro GFP oxidation when used singly, but combined inhibition of complex III and alternative oxidase by antimycin A and SHAM did cause significant oxidation. Inhibitors of complex IV and aconitase also caused oxidation of mt-ro GFP2. This oxidation was not apparent in the cytosol whereas antimycin A + SHAM also caused oxidation of cytosolic roGOFP2. Menadione had a much greater effect than the inhibitors, causing nearly complete oxidation of roGFP2 in both mitichondria and cytosol. A range of severe abiotic stress treatments (heat, salt, and heavy metal stress) led to oxidation of mt-roGFP2 while hyperosmotic stress had no effect and low temperature caused a slight but significant decrease in oxidation. Similar changes were observed for cytosolic roGFP2. Finally, the recovery of oxidation state of roGFP in mitochondria after oxidation by H₂O₂ treatment was dramatically slower than that of either the cytosol or chlorplast. Together, the results highlight the sensitivity of the mitochondrion to redox perturbation and suggest a potential role in sensing and signalling cellular redox challenge.The full-text of this article is not available in ORA, but you may be able to access the article via the publisher copy link on this record page

Effects of 2100 MHz radio frequency radiation on the viscosity of blood and oxidative stress parameters in hypertensive and normal rats

WOS: 000458419600005Background: With the development of technology, human beings are increasingly under the exposure of Radio Frequency Radiation (RFR) (due to wireless technologies, mobile phones, base stations etc). The aim was to investigate the possible effects of Radio Frequency Radiation (RFR) on blood, aorta and heart tissue in hypertensive and non-hypertensive rats with various parameters. Materials and Methods: Male Wistar Albino rats were divided into 4 groups: 1.Control (C), 2.Hypertension (H), 3.RFR, 4.RFR+Hypertension (RFR+H). 60 mg/kg L-Nitroarginine Methyl Ester (L-NAME) was administered for 1 month by oral gavage to induce hypertension. The rats were exposed to 2100 MHz RFR for 60 minutes/day, 5 days/week for 8 weeks. Blood hematocrit, blood and plasma viscosity, malondialdehyde (MDA), total nitric oxide (NOX), and Total sulphydril groups (RSH) / glutathione (GSH) levels in plasma and heart tissue were determined. Histological examination of the aortic wall and left ventricular muscle was performed. Results: RFR exposure resulted in a marked increase in cardiac and plasma MDA and NOX levels and a decrease in RSH/GSH levels (p<0.05). Blood viscosity increased in the hypertensive group with RFR exposure (p<0.05). There was an increase in left ventricular weight and number of muscle cells in hypertensive groups (p<0.05). Conclusion: According Our study suggests that exposure to RFR causes more negative effects on hypertension in terms of oxidative stress and antioxidants in the heart and plasma. Also, RFR exposure showed more prominent left ventricular fibrosis and aortic degenerative effects in hypertensive groups

The effect of non-ionizing radiation on the ovarian reserves of female rats

Objective: The authors aimed to investigate whether there was any effect of 1800 MHz GSM-modulated radio frequency radiation (RFR) as a source of non-ionizing radiation on the ovarian reserves of female rats by hematoxylin eosin staining under a light microscope, and also to evaluate the effect on the anti-Mulllerian hormone (AMH) level of rats. Design: A prospective observational study. Materials and Methods: A total of 12 age-matched young adult female Wistar albino rats were divided into two groups. Group 1 (n=6) constituted the controls; group 2 (n=6) constituted the 1800 MHz exposed animals. RFR exposed group were kept ten cm away from the horn antenna to satisfy the near field condition. The control group was kept in the same setting without any RFR exposure. The exposure period was 20 minutes for five days/week for one month. Results: The results of this study showed that 1800 MHz RFR did not have a significant effect on the ratios of atretic follicles in rat ovary tissues when compared with the control group (p>0.05). However, the authors detected statistically significantly higher AMH levels in RFR exposed groups (p<0.005). Conclusions: 1800 MHz GSM modulated RFR exposure in rats was found to have no adverse effect on ovarian reserves or follicles. The present authors' failure to detect any changes could be due to the limited duration of the RFR exposure or the limited number of subjects used in the study. AMH levels were significantly higher, which might be due to the aforementioned limitations in this study. There is a need for further experimental studies in which the effects of RFR emitted by cellular phones can be studied

Oxidative effects of extremely low frequency magnetic field and radio frequency radiation on testes tissues of diabetic and healthy rats

With the development of technology, people are increasingly under the exposure of electromagnetic fields. Individuals with chronic diseases such as diabetes are now long-term exposed to Radio Frequency RF radiation and extremely low frequency (ELF) magnetic fields (MFs). The purpose of this present study is to investigate oxidative effects and antioxidant parameters of ELF MFs and RF radiation on testis tissue in diabetic and healthy rats. Wistar male rats were divided into 10 groups. Intraperitoneal single dose STZ (65 mg/kg) dissolved in citrate buffer (0.1M (pH 4.5)) was injected to diabetes groups. ELF MFs and RF radiation were used as an electromagnetic exposure for 20 min/day, 5 days/week for one month. Testis tissue oxidant malondialdehyde (MDA), and antioxidants glutathione (GSH), and total nitric oxide (NOx) levels were determined. The results of ANOVA and Mann Whitney tests were compared; p < 0.05 was considered significant. ELF and RF radiation resulted in an increase in testicular tissue MDA and NOx levels (p < 0.05), and caused a decrease in GSH levels (p < 0.05) in both healthy and diabetic rats, yet more distinctively in diabetic rats. The most pronounced effect was recorded in D-RF + ELF group (p < 0.005). Both radiation practices increased the oxidative stress in testis tissue while causing a decrease in antioxidant level which was more distinctive in diabetic rats (Tab. 1, Fig. 3, Ref. 30). Text in PDF www.elis.sk

Effects of 2100 MHz radio frequency radiation on ductus epididymis tissue in rats

PURPOSE: The use of mobile phones is widespread since the beginning of 1990s. A great debate exists about the possible damage that the Radio Frequency-RF radiation from mobile phones exerts on different organs. The objective of this study was to investigate the possible histopathological effects of 2100 MHz RF radiation on rat ductus epididymis tissue using a light microscopy and immunohistochemical method after one or two month exposure. MATERIALS AND METHODS: The study was performed on 36 adult Wistar albino rats. 2100 MHz RF radiation was used with a specific absorption rate (SAR) of 0.36 W/kg for 30 min/day, 6 days per week for one or two months. There were 3 groups (n=6 for each group): one month RF exposed group, two months RF exposed group, and the control group. RESULTS: At the end of the study, the structural changes in ductus epididymis tissue were evaluated. In both 2100 MHz RF exposed groups, the rat ductus epididymis sperm were not observed in some channels, a reduction in sperm density in some of the channels drew an attention. The loss of connective tissue and edematous areas were observed in cross channel interstitial connective tissue. In addition, it was observed that vascularization was highly increased with respect to the control group in cross-channel interstitial connective tissue. CONCLUSION: 2100 MHz RF exposure resulted in some structural changes in the male genital ducts of rats (Tab. 1, Fig. 5, Ref 20). Text in PDF www.elis.sk