MicroRNA-222 regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells

A number of microRNAs have been shown to regulate skeletal muscle development and differentiation. MicroRNA-222 is downregulated during myogenic differentiation and its overexpression leads to alteration of muscle differentiation process and specialized structures. By using RNA-induced silencing complex (RISC) pulldown followed by RNA sequencing, combined with in silico microRNA target prediction, we have identified two new targets of microRNA-222 involved in the regulation of myogenic differentiation, Ahnak and Rbm24. Specifically, the RNA-binding protein Rbm24 is a major regulator of muscle-specific alternative splicing and its downregulation by microRNA-222 results in defective exon inclusion impairing the production of muscle-specific isoforms of Coro6, Fxr1 and NACA transcripts. Reconstitution of normal levels of Rbm24 in cells overexpressing microRNA-222 rescues muscle-specific splicing. In conclusion, we have identified a new function of microRNA-222 leading to alteration of myogenic differentiation at the level of alternative splicing, and we provide evidence that this effect is mediated by Rbm24 protei

Polarisation-independent Bragg gratings in ion-exchanged glass channel waveguides

The polarisation dependence of Bragg gratings photowritten in ion-exchanged glass waveguides is characterised for waveguides with different mask-opening widths and burial depths. It is found that polarisation-independent gratings can be written in waveguides with a wide variation in fabrication parameters

Subsurface drip irrigated potato (Solanum tuberosum L.) with saline water under Tunisian climate

Field experiment was conducted at the Higher Institute of Agronomy of Chott Meriem (Tunisia) during the growing season (2011/2012) to investigate the effects of water quality on water‘s dynamic in soil (water potential, soilcmoisture distribution, water’s stock in soil) and water use efficiency (WUE) to produce potato (Solanum tuberosum L.). Irrigation management treatments were fresh (1 dS m-1) and saline waters (4 dS m-1). Subsurface drip irrigation was used, a rate of 4 L h-1 applied at the same irrigation duration and interval. The results indicate that water content is more uniform using the saline water varies from 15 to 23% than treatment varies from 10 to 26%. The recorded changes occurred due to improving soil water distribution in root zone are explained by the increased salinity and the existence of a root system more intense level of treatment. Water quality has no direct effect on water use efficinency

Effect of different saline Levels and Irrigation Regimes on agronomic parameters of potatoes crop under the semi-arid environment of Tunisia

In Tunisia, the scarcity of water for irrigation allows the use of low water quality. Deficit irrigation has shown successful results with a large number of crops in various countries. Field experiments, were carried out in Central TunIsia (10.5632°W; 35.9191°N; 19 m a.s.l) on a potatoes crop planted in a sandy loam soil, during the growing season 2015, from January 21 (plantation of tubers) to May 21 (harvesting). Objective of the work, was to assess four different irrigation management on the agronomic parameters of potatoes crop and Water use efficient. The experimental field were laid out in a randomized complete block design consisting of two different irrigation levels and two water qualities. Each plot received the same plant management and was irrigated by a subsurface drip irrigation system (SDI). The first plot irrigated with water characterized by electrical conductivity of 1.0 dS/ m T1 with 100% ET0, T2 with 50% ET0, the second plot was irrigated with water with a water of 4dS/m and was compound by T3 with 100% ET0 and T4 with 50% ET0. This study was aimed to investigate on the effect of salt stress or water stress and the combination salt water stress on some agronomics parameters of potatoes. Statistical analysis evidenced that the degree of salinity has a highly significant positive effect on the plant’s growth, leaf area, tuber yield and volume efficiency, and water use efficiency

Using Hydrus 2-D to assess the emitters optimal position for Eggplants under surface and subsurface drip irrigation

The main objective of the work is to assess the emitters optimal position for Eggplant crop (Solanum melongena L.) in a sandy loam soil irrigated with surface or subsurface drip irrigation systems, by means of field measurements and simulations carried out with Hydrus-2D model. Initially, the performance of the model is evaluated on the basis of the comparison between simulated soil water contents (SWC) and the corresponding measured in two plots, in which laterals with coextruded emitters are laid on the soil surface (T0) and at 20 cm depth (T20), respectively. In order to choose the best position of the lateral, the results of different simulation runs, carried out by changing the installation depth of the lateral (5 cm, 15 cm and 45 cm) were compared in terms of ratio between actual transpiration and total amount of water provided during the entire growing season (WUE). Experiments were carried out, from April to June 2007, at Institut Sup\ue9rieur Agronomique de Chott M\ue9riem (Sousse, Tunisia). In the two plots, plants were spaced 0.40 m along the row and 1.2 m between the rows. Each plot was irrigated by means of laterals with coextruded emitters spaced 0.40 m and discharging a flow rate equal to 4.0 l h-1 at a nominal pressure of 100 kPa. In each plot, spatial and temporal variability of SWCs were acquired with a Time Domain Reflectometry probe (Trime-FM3), on a total of four 70 cm long access tubes, installed along the direction perpendicular to the plant row, at distances of 0, 20, 40 and 60 cm from the emitter. Irrigation water was supplied, accounting for the rainfall, every 7-10 days at the beginning of the crop cycle (March-April) and approximately once a week during the following stages till the harvesting (May-June), for a total of 15 one-hour watering. To run the model, soil evaporation, Ep, and crop transpiration, Tp were determined according to the modified FAO Penman-Monteith equation and the dual crop coefficient approach, whereas soil hydraulics and rooting system parameters were experimentally determined. Simulated SWCs resulted fairly close to the corresponding measured at different distances from the emitter and therefore the model was able to predict SWCs in the root zone with values of the Root Mean Square Error generally lower than 4%. This result is consequent to the appropriate schematization of the root distribution, as well as of the root water uptake. Simulations also evidenced the contribute of soil evaporation losses when laterals are installed from the soil surface to a 20 cm depth, whereas significant water losses by deep percolation occured at the highest installation depth. The values of WUE associated to the different examined installation depths tend to a very slight increase when the position of the lateral rises from 0 to 15 cm and start to decrease for the higher depths

Water extract of Cryphaea heteromalla (Hedw.) D. Mohr bryophyte as a natural powerful source of biologically active compounds

Bryophytes comprise of the mosses, liverworts, and hornworts. Cryphaea heteromalla, (Hedw.) D. Mohr, is a non-vascular lower plant belonging to mosses group. To the date, the most chemically characterized species belong to the liverworts, while only 3.2% and 8.8% of the species belonging to the mosses and hornworts, respectively, have been investigated. In this work, we present Folin–Ciocalteu and oxygen radical absorbance capacity (ORAC) data related to crude extracts of C. heteromalla obtained by three different extraction solvents: pure water (WT), methanol:water (80:20 v/v) (MET), and ethanol:water (80:20 v/v) (ETH). The water extract proved to be the best solvent showing the highest content of biophenols and the highest ORAC value. The C. heteromalla-WT extract was investigated by HPLC-TOF/MS (High Performance Liquid Chromatography-Time of Flight/Mass Spectrometry) allowing for the detection of 14 compounds, five of which were phenolic compounds, derivatives of benzoic, caffeic, and coumaric acids. Moreover, the C. heteromalla WT extract showed a protective effect against reactive oxygen species (ROS) generation induced by tert-butyl hydroperoxide (TBH) on the murine NIH-3T3 fibroblast cell line

Type VI secretion system mutations reduced competitive fitness of classical Vibrio cholerae biotype

The gram-negative bacterium Vibrio cholerae is the causative agent of the diarrhoeal disease cholera and is responsible for seven recorded pandemics. Several factors are postulated to have led to the decline of 6th pandemic classical strains and the rise of El Tor biotype V. cholerae, establishing the current 7th pandemic. We investigated the ability of classical V. cholerae of the 2nd and 6th pandemics to engage their type six secretion system (T6SS) in microbial competition against non-pandemic and 7th pandemic strains. We report that classical V. cholerae underwent sequential mutations in T6SS genetic determinants that initially exposed 2nd pandemic strains to microbial attack by non-pandemic strains and subsequently caused 6th pandemic strains to become vulnerable to El Tor biotype V. cholerae intraspecific competition. The chronology of these T6SS-debilitating mutations agrees with the decline of 6th pandemic classical strains and the emergence of 7th pandemic El Tor V. cholerae