25 research outputs found
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Understanding sporulation and dissemination of Podosphaera macularis, hop powdery mildew
Podosphaera macularis causes one of the most important diseases, powdery mildew, of Humulus lupulus (hop). If left unmanaged, hop powdery mildew can cause total crop loss due to disease or browning of hop cones rendering the cones unmarketable to buyers. The Hop Powdery Mildew Infection Risk Index (HOPS) is heavily relied on for management of the disease. However, HOPS assumes constant inoculum presence, which is not likely to be true. Evidence suggests that temperature fluctuations influence inoculum availability. As such, knowledge of inoculum presence and density and their relationship to environmental conditions may increase management efficacy by reducing pesticide use and cost of crop production.
Molecular methods for detecting and quantifying P. macularis were developed and used to analyze air samples taken from hop fields for two seasons. A protocol for DNA extraction from field samples was modified in order to use PCR to detect and quantify P. macularis DNA from the field. Primers and a probe designed to the internal
transcribed spacer (ITS) region of P. macularis ribosomal DNA have made inoculum detection possible using both conventional polymerase chain reaction (PCR) and real-time quantitative PCR (qPCR). DNA extracted from conidia impinged on glass rods coated in silicon grease has been used as template for qPCR, and we have successfully detected as DNA extracted from 10 spores Specific and sensitive detection of P. macularis DNA with conventional PCR was possible under conditions typically used to evaluate qPCR; however results from field samples indicated that sensitivity was an issue, in that spores were not detected in samples from fields with high disease pressure.
The effects of constant exposure (5, 10, 15, 20, 25, 30, and 35°C) and 6 hr exposure (18, 22, 26, 30, 34, and 38°C) on sporulation of P. macularis were tested. Exposure to constant low and high temperatures decreases sporulation, which indicates that inoculum may not always be available once the epidemic has started. Sporulation is also decreased during brief exposures to temperatures above 30°C. These data indicate that inoculum availability is reduced when the temperature exceeds 30°C in the field. A modification of HOPS should be examined to incorporate an algorithm which takes the findings in this study into account
Comparative Genomics of Wolbachia–Cardinium Dual Endosymbiosis in a Plant-Parasitic Nematode
Wolbachia and Cardinium are among the most important and widespread of all endosymbionts, occurring in nematodes and more than half of insect and arachnid species, sometimes as coinfections. These symbionts are of significant interest as potential biocontrol agents due to their abilities to cause major effects on host biology and reproduction through cytoplasmic incompatibility, sex ratio distortion, or obligate mutualism. The ecological and metabolic effects of coinfections are not well understood. This study examined a Wolbachia–Cardinium coinfection in the plant-parasitic nematode (PPN), Pratylenchus penetrans, producing the first detailed study of such a coinfection using fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR), and comparative genomic analysis. Results from FISH and single-nematode PCR showed 123/127 individuals in a focal population carried Cardinium (denoted strain cPpe), and 48% were coinfected with Wolbachia strain wPpe. Both endosymbionts showed dispersed tissue distribution with highest densities in the anterior intestinal walls and gonads. Phylogenomic analyses confirmed an early place of cPpe and long distance from a sister strain in another PPN, Heterodera glycines, supporting a long history of both Cardinium and Wolbachia in PPNs. The genome of cPpe was 1.36 Mbp with 35.8% GC content, 1,131 predicted genes, 41% having no known function, and missing biotin and lipoate synthetic capacity and a plasmid present in other strains, despite having a slightly larger genome compared to other sequenced Cardinium. The larger genome revealed expansions of gene families likely involved in host–cellular interactions. More than 2% of the genes of cPpe and wPpe were identified as candidate horizontally transferred genes, with some of these from eukaryotes, including nematodes. A model of the possible Wolbachia–Cardinium interaction is proposed with possible complementation in function for pathways such as methionine and fatty acid biosynthesis and biotin transport
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Using Mitogenomic and Nuclear Ribosomal Sequence Data to Investigate the Phylogeny of the Xiphinema americanum Species Complex
Nematodes within the Xiphinema americanum species complex are economically important because they vector nepoviruses which cause considerable damage to a variety of agricultural crops. The taxonomy of X. americanum species complex is controversial, with the number of putative species being the subject of debate. Accurate phylogenetic knowledge of this group is highly desirable as it may ultimately reveal genetic differences between species. For this study, nematodes belonging to the X. americanum species complex, including potentially mixed species populations, were collected from 12 geographically disparate locations across the U.S. from different crops and in varying association with nepoviruses. At least four individuals from each population were analyzed. A portion of the 18S nuclear ribosomal DNA (rDNA) gene was sequenced for all individuals while the internal transcribed spacer region 1 (ITS1) of rDNA was cloned and 2 to 6 clones per individual were sequenced. Mitochondrial genomes for numerous individuals were sequenced in parallel using high-throughput DNA sequencing (HTS) technology. Phylogenetic analysis of the 18S rDNA revealed virtually identical sequences across all populations. Analysis of ITS1 rDNA sequences revealed several well-supported clades, with some degree of congruence with geographic location and viral transmission, but also numerous presumably paralogous sequences that failed to form clades with other sequences from the same population. Analysis of mitochondrial DNA (mtDNA) indicated the presence of three distinct monophyletic clades of X. americanum species complex nematodes. Two clades contained nematodes found in association with nepovirus and the third contained divergent mtDNA sequences from three nematode populations from the western U.S. where nepovirus was absent. The inherent heterogeneity in ITS1 rDNA sequence data and lack of informative sites in 18S rDNA analysis suggests that mtDNA may be more useful in sorting out the taxonomic confusion of the X. americanum species complex
Variable Abundance and Distribution of Wolbachia and Cardinium Endosymbionts in Plant-Parasitic Nematode Field Populations
The bacterial endosymbiont Wolbachia interacts with different invertebrate hosts, engaging in diverse symbiotic relationships. Wolbachia is often a reproductive parasite in arthropods, but an obligate mutualist in filarial nematodes. Wolbachia was recently discovered in plant-parasitic nematodes, and, is thus far known in just two genera Pratylenchus and Radopholus, yet the symbiont’s function remains unknown. The occurrence of Wolbachia in these economically important plant pests offers an unexplored biocontrol strategy. However, development of Wolbachia-based biocontrol requires an improved understanding of symbiont-host functional interactions and the symbiont’s prevalence among nematode field populations. This study used a molecular-genetic approach to assess the prevalence of a Wolbachia lineage (wPpe) in 32 field populations of Pratylenchus penetrans. Populations were examined from eight different plant species in Washington, Oregon, and California. Nematodes were also screened for the endosymbiotic bacterium Cardinium (cPpe) that was recently shown to co-infect P. penetrans. Results identified wPpe in 9/32 and cPpe in 1/32 of P. penetrans field populations analyzed. No co-infection was observed in field populations. Wolbachia was detected in nematodes from 4/8 plant-hosts examined (raspberry, strawberry, clover, and lily), and in all three states surveyed. Cardinium was detected in nematodes from mint in Washington. In the wPpe-infected P. penetrans populations collected from raspberry, the prevalence of wPpe infection ranged from 11 to 58%. This pattern is unlike that in filarial nematodes where Wolbachia is an obligate mutualist and occurs in 100% of the host. Further analysis of wPpe-infected populations revealed female-skewed sex ratios (up to 96%), with the degree of skew positively correlating with wPpe prevalence. Uninfected nematode populations had approximately equal numbers of males and females. Comparisons of 54 wPpe 16S ribosomal RNA sequences revealed high similarity across the geographic isolates, with 45 of 54 isolates being identical at this locus. The complete absence of wPpe among some populations and low prevalence in others suggest that this endosymbiont is not an obligate mutualist of P. penetrans. The observed sex ratio bias in wPpe-infected nematode populations is similar to that observed in arthropods where Wolbachia acts as a reproductive manipulator, raising the question of a similar role in plant-parasitic nematodes
Cereal Root Interactions with Soilborne Pathogens—From Trait to Gene and Back
Realizing the yield potential of crop plants in the presence of shifting pathogen populations, soil quality, rainfall, and other agro-environmental variables remains a challenge for growers and breeders worldwide. In this review, we discuss current approaches for combatting the soilborne phytopathogenic nematodes, Pratylenchus and Heterodera of wheat and barley, and Meloidogyne graminicola Golden and Birchfield, 1965 of rice. The necrotrophic fungal pathogens, Rhizoctonia solani Kühn 1858 AG-8 and Fusarium spp. of wheat and barley, also are discussed. These pathogens constitute major causes of yield loss in small-grain cereals of the Pacific Northwest, USA and throughout the world. Current topics include new sources of genetic resistance, molecular leads from whole genome sequencing and genome-wide patterns of hosts, nematode or fungal gene expression during root-pathogen interactions, host-induced gene silencing, and building a molecular toolbox of genes and regulatory sequences for deployment of resistance genes. In conclusion, improvement of wheat, barley, and rice will require multiple approaches
Using mitogenomic and nuclear ribosomal sequence data to investigate the phylogeny of the Xiphinema americanum species complex.
Nematodes within the Xiphinema americanum species complex are economically important because they vector nepoviruses which cause considerable damage to a variety of agricultural crops. The taxonomy of X. americanum species complex is controversial, with the number of putative species being the subject of debate. Accurate phylogenetic knowledge of this group is highly desirable as it may ultimately reveal genetic differences between species. For this study, nematodes belonging to the X. americanum species complex, including potentially mixed species populations, were collected from 12 geographically disparate locations across the U.S. from different crops and in varying association with nepoviruses. At least four individuals from each population were analyzed. A portion of the 18S nuclear ribosomal DNA (rDNA) gene was sequenced for all individuals while the internal transcribed spacer region 1 (ITS1) of rDNA was cloned and 2 to 6 clones per individual were sequenced. Mitochondrial genomes for numerous individuals were sequenced in parallel using high-throughput DNA sequencing (HTS) technology. Phylogenetic analysis of the 18S rDNA revealed virtually identical sequences across all populations. Analysis of ITS1 rDNA sequences revealed several well-supported clades, with some degree of congruence with geographic location and viral transmission, but also numerous presumably paralogous sequences that failed to form clades with other sequences from the same population. Analysis of mitochondrial DNA (mtDNA) indicated the presence of three distinct monophyletic clades of X. americanum species complex nematodes. Two clades contained nematodes found in association with nepovirus and the third contained divergent mtDNA sequences from three nematode populations from the western U.S. where nepovirus was absent. The inherent heterogeneity in ITS1 rDNA sequence data and lack of informative sites in 18S rDNA analysis suggests that mtDNA may be more useful in sorting out the taxonomic confusion of the X. americanum species complex
Genome Skimming: A Rapid Approach to Gaining Diverse Biological Insights into Multicellular Pathogens
<p>Genome Skimming: A Rapid Approach to Gaining Diverse Biological Insights into Multicellular Pathogens</p
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HoweDanaZoologyUsingMitogenomicNuclear.pdf
Nematodes within the Xiphinema americanum species complex are economically important because they vector
nepoviruses which cause considerable damage to a variety of agricultural crops. The taxonomy of X. americanum species
complex is controversial, with the number of putative species being the subject of debate. Accurate phylogenetic
knowledge of this group is highly desirable as it may ultimately reveal genetic differences between species. For this study,
nematodes belonging to the X. americanum species complex, including potentially mixed species populations, were
collected from 12 geographically disparate locations across the U.S. from different crops and in varying association with
nepoviruses. At least four individuals from each population were analyzed. A portion of the 18S nuclear ribosomal DNA
(rDNA) gene was sequenced for all individuals while the internal transcribed spacer region 1 (ITS1) of rDNA was cloned and
2 to 6 clones per individual were sequenced. Mitochondrial genomes for numerous individuals were sequenced in parallel
using high-throughput DNA sequencing (HTS) technology. Phylogenetic analysis of the 18S rDNA revealed virtually identical
sequences across all populations. Analysis of ITS1 rDNA sequences revealed several well-supported clades, with some
degree of congruence with geographic location and viral transmission, but also numerous presumably paralogous
sequences that failed to form clades with other sequences from the same population. Analysis of mitochondrial DNA
(mtDNA) indicated the presence of three distinct monophyletic clades of X. americanum species complex nematodes. Two
clades contained nematodes found in association with nepovirus and the third contained divergent mtDNA sequences from
three nematode populations from the western U.S. where nepovirus was absent. The inherent heterogeneity in ITS1 rDNA
sequence data and lack of informative sites in 18S rDNA analysis suggests that mtDNA may be more useful in sorting out
the taxonomic confusion of the X. americanum species complex