1,082 research outputs found
Excellent daytime seeing at Dome Fuji on the Antarctic plateau
Context. Dome Fuji, the second highest region on the Antarctic plateau, is
expected to have some of the best astronomical seeing on Earth. However, site
testing at Dome Fuji is still in its very early stages.
Aims. To investigate the astronomical seeing in the free atmosphere above
Dome Fuji, and to determine the height of the surface boundary layer.
Methods. A Differential Image Motion Monitor was used to measure the seeing
in the visible (472 nm) at a height of 11 m above the snow surface at Dome Fuji
during the austral summer of 2012/2013.
Results. Seeing below 0.2'' has been observed. The seeing often has a local
minimum of ~0.3'' near 18 h local time. Some periods of excellent seeing, 0.3''
or smaller, were also observed, sometimes extending for several hours at local
midnight. The median seeing is higher, at 0.52''---this large value is believed
to be caused by periods when the telescope was within the turbulent boundary
layer.
Conclusions. The diurnal variation of the daytime seeing at Dome Fuji is
similar to that reported for Dome C, and the height of the surface boundary
layer is consistent with previous simulations for Dome Fuji. The free
atmosphere seeing is ~0.2'', and the height of the surface boundary layer can
be as low as ~11 m.Comment: 4 pages, 6 figures, Submitted to Astronomy & Astrophysics (letter
The deoxyribonucleic acid polymerases from the diatom Cylindrotheca fusiformis. Subcellular distribution, exonuclease activity and heterogeneity of the enzymes
Sex-biased inbreeding effects impact upon reproductive success and home range size in the critically endangered black rhinoceros
A reliable, single stage method for gender determination in black rhinoceros from low-copy template DNA
Evidence for a companion to BM Gem, a silicate carbon star
Balmer and Paschen continuum emission as well as Balmer series lines of P
Cygni-type profile from H_gamma through H_23 are revealed in the violet spectra
of BM Gem, a carbon star associated with an oxygen-rich circumstellar shell
(`silicate carbon star') observed with the high dispersion spectrograph (HDS)
on the Subaru telescope. The blue-shifted absorption in the Balmer lines
indicates the presence of an outflow, the line of sight velocity of which is at
least 400 km s^-1, which is the highest outflow velocity observed to date in a
carbon star. We argue that the observed unusual features in BM Gem are strong
evidence for the presence of a companion, which should form an accretion disk
that gives rise to both an ionized gas region and a high velocity, variable
outflow. The estimated luminosity of ~0.2 (0.03-0.6) L_sun for the ionized gas
can be maintained by a mass accretion rate to a dwarf companion of ~10^-8 M_sun
yr^-1, while ~10^-10 M_sun yr^-1 is sufficient for accretion to a white dwarf
companion. These accretion rates are feasible for some detached binary
configurations on the basis of the Bond-Hoyle type accretion process. We
concluded that the carbon star BM Gem is in a detached binary system with a
companion of low mass and low luminosity. However, we are unable to determine
whether this companion object is a dwarf or a white dwarf. The upper limits for
binary separation are 210 AU and 930 AU for a dwarf and a white dwarf,
respectively. We also note that the observed features of BM Gem mimic those of
Mira (omi Cet), which may suggest actual similarities in their binary
configurations and circumstellar structures.Comment: 11 pages, 2 figures, 1 table, accepted for publication in Ap
Virus-free induction of pluripotency and subsequent excision of reprogramming factors
Reprogramming of somatic cells to pluripotency, thereby creating induced pluripotent stem (iPS) cells, promises to transform regenerative medicine. Most instances of direct reprogramming have been achieved by forced expression of defined factors using multiple viral vectors1-7. However, such iPS cells contain a large number of viral vector integrations1,8, any one of which could cause unpredictable genetic dysfunction. While c-Myc is dispensable for reprogramming9,10, complete elimination of the other exogenous factors is also desired since ectopic expression of either Oct4 or Klf4 can induce dysplasia11,12. Two transient transfection reprogramming methods have been published to address this issue13,14. However, the efficiency of either approach is extremely low, and neither has thus far been applied successfully to human cells. Here we show that non-viral transfection of a single multiprotein expression vector, which comprises the coding sequences of c-Myc, Klf4, Oct4 and Sox2 linked with 2A peptides, can reprogram both mouse and human fibroblasts. Moreover, the transgene can be removed once reprogramming has been achieved. iPS cells produced with this non-viral vector show robust expression of pluripotency markers, indicating a reprogrammed state confirmed functionally by in vitro differentiation assays and formation of adult chimeric mice. When the single vector reprogramming system was combined with a piggyBac transposon15,16 we succeeded in establishing reprogrammed human cell lines from embryonic fibroblasts with robust expression of pluripotency markers. This system minimizes genome modification in iPS cells and enables complete elimination of exogenous reprogramming factors, efficiently providing iPS cells that are applicable to regenerative medicine, drug screening and the establishment of disease models
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair.
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair outcomes. Here, we utilize a pooled CRISPR screen to define host cell involvement in HDR between a Cas9 DSB and a plasmid double stranded donor DNA (dsDonor). We find that the Fanconi Anemia (FA) pathway is required for dsDonor HDR and that other genes act to repress HDR. Small molecule inhibition of one of these repressors, CDC7, by XL413 and other inhibitors increases the efficiency of HDR by up to 3.5 fold in many contexts, including primary T cells. XL413 stimulates HDR during a reversible slowing of S-phase that is unexplored for Cas9-induced HDR. We anticipate that XL413 and other such rationally developed inhibitors will be useful tools for gene modification
Chemical composition of carbon-rich, very metal-poor subgiant LP625-44 observed with the Subaru/HDS
We have obtained a high resolution (R~90,000) spectrum of the carbon- and
s-process-element-rich, very metal-poor ([Fe/H]=-2.7) subgiant LP625-44, as
well as that of HD140283 (a metal-poor subgiant with normal abundance ratio)
for comparison, with the High Dispersion Spectrograph (HDS) for the Subaru
Telescope for detailed abundance study. The oxygen abundance derived from the O
I triplet around 7770A is uncertain, but the excess of oxygen in LP625-44 seems
remarkable (perhaps by nearly a factor 10), in comparison with that of HD140283
derived from the same lines. The Na enhancement in LP625-44 is by about a
factor 50, suggesting hydrogen burning in the 22Ne-rich layer in an asymptotic
giant branch star which produces the abundance pattern of this object. In our
new spectrum of LP625-44, the Pb I lambda 3683A line has been detected, as well
as the Pb I lambda 4057A line which has already been studied, confirming the Pb
abundance (log epsilon(Pb)~1.9) derived by the previous work. The abundance
ratio of s-process elements at the second peak (e.g., La, Ce and Nd) to that at
the third peak (Pb) in LP 625-44 is significantly higher (by a factor 5) than
that in other three s-process element-rich objects recently studied by van Eck
et al.. Recent theoretical works have modeled the s-process nucleosynthesis in
the radiative layer of asymptotic giant branch stars in the inter-pulse phase,
and the above results means that these processes produced a large scatter in
the abundance ratios. Another possiblity is that different processes (e.g.,
s-process nucleosynthesis during thermal pulses) have contributed to heavy
elements in the early Galaxy.Comment: 65 pages, 11 figures, to appear in PAS
Heteroepitaxial growth of ferromagnetic MnSb(0001) films on Ge/Si(111) virtual substrates
Molecular beam epitaxial growth of ferromagnetic MnSb(0001) has been achieved on high quality, fully relaxed Ge(111)/Si(111) virtual substrates grown by reduced pressure chemical vapor deposition. The epilayers were characterized using reflection high energy electron diffraction, synchrotron hard X-ray diffraction, X-ray photoemission spectroscopy, and magnetometry. The surface reconstructions, magnetic properties, crystalline quality, and strain relaxation behavior of the MnSb films are similar to those of MnSb grown on GaAs(111). In contrast to GaAs substrates, segregation of substrate atoms through the MnSb film does not occur, and alternative polymorphs of MnSb are absent
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