17 research outputs found
An apoplastic fluid extraction method for the characterization of grapevine leaves proteome and metabolome from a single sample
The analysis of complex biological systems keeps challenging
researchers. The main goal of systems biology is to decipher interactions
within cells, by integrating datasets from large scale analytical
approaches including transcriptomics, proteomics and metabolomics
andmore specialized âOMICSâ such as epigenomics and lipidomics. Studying
different cellular compartments allows a broader understanding of cell
dynamics. Plant apoplast, the cellular compartment external to the plasma
membrane including the cell wall, is particularly demanding to analyze.
Despite our knowledge on apoplast involvement on several processes from
cell growth to stress responses, its dynamics is still poorly known due to the
lack of efficient extraction processes adequate to each plant system.Analyzing
woody plants such as grapevine raises even more challenges. Grapevine is
among the most important fruit crops worldwide and awider characterization
of its apoplast is essential for a deeper understanding of its physiology and cellular
mechanisms. Here, we describe, for the first time, a vacuum-infiltrationcentrifugationmethod
that allows a simultaneous extraction of grapevine apoplastic
proteins and metabolites from leaves on a single sample, compatible
with high-throughput mass spectrometry analyses. The extracted apoplast
from two grapevine cultivars, Vitis vinifera cv âTrincadeiraâ and âRegentâ, was
directly used for proteomics and metabolomics analysis. The proteome was
analyzed by nanoLC-MS/MS and more than 700 common proteinswere identified,
with highly diverse biological functions. The metabolome profile
through FT-ICR-MS allowed the identification of 514 unique putative compounds
revealing a broad spectrum of molecular classesinfo:eu-repo/semantics/publishedVersio
Television pictures of Phobos: first results
In February-March 1989, 37 television images of the Martian satellite Phobos were obtained by the Phobos 2 spacecraft from distances of 200-1100 km. These images provide an important supplement to the TV data from the American Mariner 9 and Viking spacecraft in coverage of t4e surface of Phobos and in resolution in certain regions, in spectral range, and in range of phase angles. They make it possible to refine the figure and topographic and geological maps of the surface of Phobos, its spectral and angular reflective characteristics,
the surface composition and texture, and characteristics of the orbital and librational motion
Rivulet/Droplet Dynamics and Boiling Incipience in Microgravity: Results of ESA Parabolic Flights Experiments
Two-phase flows are present in space applications: life
support systems, electronic cooling devices, energy
production and, as one can expect in the future, in waste
water treatment for long duration space exploration missions.
In many applications high heat fluxes are achieved by
utilising the latent heat associated with the phase change
from liquid to vapour. However, the physical mechanisms
involved are intricate and many questions remain
unanswered. Three independent experiments have been
conducted during the 52nd ESA Parabolic Flights Campaign
of May 2010 using a common apparatu
Rivulet/Droplet Dynamics and Boiling Incipience in Microgravity: Results of ESA Parabolic Flights Experiments
Book of Abstractsinfo:eu-repo/semantics/publishe