Analysis of a database of DNA profiles of 734 hybrid tea rose varieties

Over 10,000 Hybrid Tea rose varieties have been described. The large number of varieties and the size of the reference collections may cause problems in DUS (Distinctiveness, Uniformity, and Stability) testing. Molecular markers may help to solve these problems by providing high power to identify and recognise seedling-derived varieties based on unique genotypes, while grouping mutants into groups with identical marker scores. Here we describe the use of a set of 11 rose microsatellite markers to generate a database of molecular profiles of Hybrid Tea varieties. The data were analysed with respect to reproducibility, discriminative power, genetic (sub) structure, and correlation between molecular and DUS characteristics. The use of the markers in the DUS context is discussed with respect to the options 2 and 3 as they were formulated by the UPOV-BMT working group. It is concluded that an option 3 approach (granting of PBR based on distinctness observed with molecular markers) is feasible for ros

Diversity between and within farmers’ varieties of tomato from Eritrea

Tomato yields in Eritrea are low (15 Mg/ha) compared with 19 Mg/ha in Africa and 27 Mg/ha worldwide. This is partly caused by poor quality of varieties used. This study analysed the diversity among and heterogeneity within farmers’ varieties of tomato from Eritrea and compared these varieties with other African and Italian varieties. Fifteen simple sequence repeat (SSR) markers were used for the genetic analysis. Genetic similarities among the varieties were calculated and an Unweighted Pair Group Method with Arithmetic Mean analysis was performed. Furthermore, individual plants of varieties were genotyped to evaluate uniformity within varieties. A high degree of diversity was observed among the Eritrean varieties. Thirteen out of the 15 SSRs were polymorphic, with 2 to 5 alleles per marker. The dendrogram showed two major types of varieties: San-Marzano and Marglob. Eritrean varieties were closely related to old Italian varieties in both types. Analysis of the within-variety variation showed that the Eritrean tomato genotypes were less uniform than the other varieties, probably because of deliberate mixing. A survey among farmers showed that some of them purposely mixed seeds to prolong the harvesting period, for yield stability and stress tolerance. Farmers value ‘new material’ as a source of influ

Defects and agility: localization issues in agile development projects

It has been noted that software localization does not always fit well into agile software development. This poster aims to illustrate the relationship between the two by examining how localization issues occur in agile projects. A list of common localization issues is presented and examined as to where and why they can be caused during development and if there is a connection to agile methodologies. The poster serves as an introduction to our research in this area

A European reference collection of rose varieties : final report

An integrated pilot database was constructed containing administrative, morphological and molecular data as well as pictures of each variety. In spite of some encountered difficulties, it was demonstrated that two laboratories can produce substantially equivalent data and that the molecular data produced is useful as a tool for managing reference collections, prescreening and quality assuranc