A Full MALDI-Based Approach to Detect Plasmid-Encoded KPC-Producing Klebsiella pneumoniae

KPC-producing Klebsiella pneumoniae represents a severe public health concern worldwide. The rapid detection of these isolates is of fundamental importance for the adoption of proper antibiotic treatment and infection control measures, and new applications of MALDI-TOF MS technology fit this purpose. In this study, we present a full MALDI-based approach to detect plasmid-encoded KPC-producing strains, accomplished by the automated detection of a KPC-specific peak (at 11,109 m/z) by a specific algorithm integrated into the MALDI Biotyper system (Bruker Daltonik), and the confirmation of carbapenemase activity by STAR-Carba imipenem hydrolysis assay. A total of 6209 K. pneumoniae isolates from Italy and Germany were investigated for the presence of the KPC-related peak, and a subset of them (n = 243) underwent confirmation of carbapenemase activity by STAR-Carba assay. The novel approach was further applied directly to positive blood culture bottles (n = 204), using the bacterial pellet obtained with Sepsityper kit (Bruker Daltonik). The novel approach enabled a reliable and very fast detection of KPC-producing K. pneumoniae strains, from colonies as well as directly from positive blood cultures. The automated peak detection enabled the instant detection of KPC-producing K. pneumoniae during the routine identification process, with excellent specificity (100%) and a good sensitivity (85.1%). The sensitivity is likely mainly related to the prevalence of the specific plasmid harboring clones among all the KPC-producing circulating strains. STAR-Carba carbapenemase confirmation showed 100% sensitivity and specificity, both from colonies and from positive blood cultures

P-EdgeCoolingMode: An Agent Based Performance Aware Thermal Management Unit for DVFS Enabled Heterogeneous MPSoCs

Thermal cycling as well as spatial and thermal gradient affects the lifetime reliability and performance of heterogeneous multiprocessor systems-on-chips (MPSoCs). Conventional temperature management techniques are not intelligent enough to cater for performance, energy efficiency as well as operating temperature of the system. In this paper we propose a light-weight novel thermal management mechanism (P-EdgeCoolingMode) in the form of intelligent software agent, which monitors and regulates the operating temperature of the CPU cores to improve reliability of the system while catering for performance requirements. P-EdgeCoolingMode is capable of pro-actively monitoring performance and based on the user’s demand the agent takes necessary action, making the proposed methodology highly suitable for implementation on existing as well as conceptual Edge devices utilizing heterogeneous MPSoCs with dynamic voltage and frequency scaling (DVFS) capabilities. We validated our methodology on the Odroid-XU4 MPSoC and Huawei P20 Lite (HiSilicon Kirin 659 MPSoC). P-EdgeCoolingMode has been successful to reduce the operating temperature while improving performance and reducing power consumption for chosen test cases than the state-of-the-art. For applications with demanding performance requirement P-EdgeCoolingMode has been found to improve the power consumption by 30.62% at the most in comparison to existing state-of-the-art power management methodologies

Wettability Switching Techniques on Superhydrophobic Surfaces

The wetting properties of superhydrophobic surfaces have generated worldwide research interest. A water drop on these surfaces forms a nearly perfect spherical pearl. Superhydrophobic materials hold considerable promise for potential applications ranging from self cleaning surfaces, completely water impermeable textiles to low cost energy displacement of liquids in lab-on-chip devices. However, the dynamic modification of the liquid droplets behavior and in particular of their wetting properties on these surfaces is still a challenging issue. In this review, after a brief overview on superhydrophobic states definition, the techniques leading to the modification of wettability behavior on superhydrophobic surfaces under specific conditions: optical, magnetic, mechanical, chemical, thermal are discussed. Finally, a focus on electrowetting is made from historical phenomenon pointed out some decades ago on classical planar hydrophobic surfaces to recent breakthrough obtained on superhydrophobic surfaces

Nutritional therapy and infectious diseases: a two-edged sword

The benefits and risks of nutritional therapies in the prevention and management of infectious diseases in the developed world are reviewed. There is strong evidence that early enteral feeding of patients prevents infections in a variety of traumatic and surgical illnesses. There is, however, little support for similar early feeding in medical illnesses. Parenteral nutrition increases the risk of infection when compared to enteral feeding or delayed nutrition. The use of gastric feedings appears to be as safe and effective as small bowel feedings. Dietary supplementation with glutamine appears to lower the risk of post-surgical infections and the ingestion of cranberry products has value in preventing urinary tract infections in women

Droplet Actuation by Electrowetting-on-Dielectric (EWOD): A Review

This paper reviews publications that have fortified our understanding of the electrowetting-on-dielectric (EWOD) actuation mechanism. Over the last decade, growing interest in EWOD has led to a wide range of scientific and technological investigations motivated by its applicability in microfluidics, especially for droplet-based optical and lab-on-a-chip systems. At this point in time, we believe that it is helpful to summarize the observations, insights, and modeling techniques that have led to the current picture showing how forces act on liquid droplets and how droplets respond in EWOD microfluidic devices. We discuss the basic physics of EWOD and explain the mechanical response of a droplet using free-body diagrams. It is our hope that this review will inspire new research approaches and help design useful devices. © 2012 Copyright Taylor and Francis Group, LLC

Content of macroelements in tubers of several potato varieties depending on the foliar fertilization applied

The aim of the study was to determine the effect of foliar fertilizers containing macro- and microelements, and used in the form of chelates, on the content of macroelements in tubers of several potato varieties. The study was based on a 3-year (2013-2015) field experiment conducted in Haczow (49°39′40″N, 21°53′49″E), on brown, slightly acidic soil. The experiment was established using the method of random subblocks, where the first order factors were foliar fertilization treatments with the following fertilizers: Fortis Duotop Zn Mn + Fortis Aminotop (A), Fortis B Mo + Ferti Agro (B), Fortis Zn Mn + Fortis B Mo (C) and the control object, without foliar fertilization. The second order factors were 4 potato varieties belonging to different classes of earliness (Agnes, Jelly, Viviana, Vineta). Foliar application of all fertilizer combinations contributed to an increase in the magnesium content, while the application of Fortis B Mo + Ferti Agro fertilizers increased the nitrogen and potassium content. Genetic traits differentiated the content of macroelements. Tubers of the cultivar Jelly were characterized by the largest accumulation of phosphorus, potassium, magnesium and calcium, while the Viviana cultivar had the smallest amounts of phosphorus, potassium, calcium. The meteorological conditions in the years of the experiment modified the content of macroelements. Under the dry summer conditions but very wet September in 2013, the tubers accumulated most magnesium and calcium, but contained the least nitrogen, phosphorus and potassium. With excess precipitation in 2014, the tubers were found to contain the most phosphorus, potassium and calcium, while in the dry 2015 year, with a significant shortage of precipitation during the potato growing season and air temperature higher than the long-term average, the tubers accumulated the highest nitrogen but the lowest calcium amounts