Clonal analysis of meningococci during a 26 year period prior to the introduction of meningococcal serogroup C vaccines

Meningococcal disease remains a public health burden in the UK and elsewhere. Invasive Neisseria meningitidis, isolated in Scotland between 1972 and 1998, were characterised retrospectively to examine the serogroup and clonal structure of the circulating population. 2607 isolates causing invasive disease were available for serogroup and MLST analysis whilst 2517 were available for multilocus sequence typing (MLST) analysis only. Serogroup distribution changed from year to year but serogroups B and C were dominant throughout. Serogroup B was dominant throughout the 1970s and early 1980s until serogroup C became dominant during the mid-1980s. The increase in serogroup C was not associated with one particular sequence type (ST) but was associated with a number of STs, including ST-8, ST-11, ST-206 and ST-334. This is in contrast to the increase in serogroup C disease seen in the 1990s that was due to expansion of the ST-11 clonal complex. While there was considerable diversity among the isolates (309 different STs among the 2607 isolates), a large proportion of isolates (59.9%) were associated with only 10 STs. These data highlight meningococcal diversity over time and the need for ongoing surveillance during the introduction of new meningococcal vaccines

Measurement of the Angle of Plantar Flexion An Objective Way of Assessing Muscle Relaxation in Children with Spastic Cerebral Palsy

Infants and young children with spastic cerebral palsy (CP) do well with a single daily dose of diazepamgiven at bedtime, as the muscle relaxation decreases the spasticity and faciltates both passive and voluntarymovements. This double blind randomized control trial showed that reducing the hypertonia enhanced thechild’s mobility and quality of life without causing daytime drowsiness. The traditional method of assessingthe muscle tone is by using the Ashworth’s scale. However this is a subjective measurement and inter-rater variability is invariably present. The angle of plantar flexion at the ankle was measured in this study using a goniometer and compared with the Ashworth scores to study if it is a valid and reliable indicator of musclerelaxation. Measuring the angle of plantar flexion is easy to perform and is an effective and objective tool to monitor the dose of diazepam required for optimum muscle relaxation

Structure and lattice dynamics of the wide band gap semiconductors MgSiN2_{2} and MgGeN2_{2}

We have determined the structural and lattice dynamical properties of the orthorhombic, wide band gap semiconductors MgSiN$_{2}$ and MgGeN$_{2}$ using density functional theory. In addition, we present structural properties and Raman spectra of a MgSiN$_{2}$ powder. The structural properties and lattice dynamics of the orthorhombic systems are compared to wurtzite AlN. We find clear differences in the lattice dynamics between MgSiN$_{2}$, MgGeN$_{2}$ and AlN, for example we find that the highest phonon frequency in MgSiN$_{2}$ is about 100~cm$^{-1}$ higher than the highest frequency in AlN and that MgGeN$_{2}$ is much softer. We also provide the Born effective charge tensors and dielectric tensors of MgSiN$_{2}$, MgGeN$_{2}$ and AlN. Phonon related thermodynamic properties, such as the heat capacity and entropy, are in very good agreement with available experimental results.Comment: 9 pages, 11 figures, 6 table

Annotating Synapses in Large EM Datasets

Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience and becoming a focus of the emerging field of connectomics. To date, electron microscopy (EM) is the most proven technique for identifying and quantifying synaptic connections. As advances in EM make acquiring larger datasets possible, subsequent manual synapse identification ({\em i.e.}, proofreading) for deciphering a connectome becomes a major time bottleneck. Here we introduce a large-scale, high-throughput, and semi-automated methodology to efficiently identify synapses. We successfully applied our methodology to the Drosophila medulla optic lobe, annotating many more synapses than previous connectome efforts. Our approaches are extensible and will make the often complicated process of synapse identification accessible to a wider-community of potential proofreaders

Properties of Umbral Dots from Stray Light Corrected Hinode Filtergrams

High resolution blue continuum filtergrams from Hinode are employed to study the umbral fine structure of a regular unipolar sunspot. The removal of scattered light from the images increases the rms contrast by a factor of 1.45 on average. Improvement in image contrast renders identification of short filamentary structures resembling penumbrae that are well separated from the umbra-penumbra boundary and comprise bright filaments/grains flanking dark filaments. Such fine structures were recently detected from ground based telescopes and have now been observed with Hinode. A multi-level tracking algorithm was used to identify umbral dots in both the uncorrected and corrected images and to track them in time. The distribution of the values describing the photometric and geometric properties of umbral dots are more easily affected by the presence of stray light while it is less severe in the case of kinematic properties. Statistically, umbral dots exhibit a peak intensity, effective diameter, lifetime, horizontal speed and a trajectory length of 0.29 I_QS, 272 km, 8.4 min, 0.45 km/s and 221 km respectively. The 2 hr 20 min time sequence depicts several locations where umbral dots tend to appear and disappear repeatedly with various time intervals. The correction for scattered light in the Hinode filtergrams facilitates photometry of umbral fine structure which can be related to results obtained from larger telescopes and numerical simulations.Comment: Accepted for publication in ApJ : 10 pages, 10 figures, 3 table

Acute military psychiatric casualties from the war in Iraq

Background: The view that most military personnel evacuated from war zones are suffering from combat stress reactions, or are otherwise traumatised by the horrors of war, has an impact on all aspects of military psychiatry. Aims: To delineate the reasons for psychiatric aeromedical evacuation from Iraq from the start of build-up of UK forces in January 2003 until the end of October that year, 6 months after the end of formal hostilities. Method: A retrospective study was conducted of field and in-patient psychiatric assessments of 116 military personnel evacuated to the UK military psychiatric in-patient facility in Catterick Garrison. Results: Evacuees were mainly non-combatants (69%). A significant proportion were in reserve service (21%) and had a history of contact with mental health services (37%). Only 3% had a combat stress reaction. In over 85% of cases evacuation was for low mood attributed to separation from friends or family, or difficulties adjusting to the environment. Conclusions: These findings have implications especially for screening for suitability for deployment, and for understanding any longer-term mental health problems arising in veterans from Iraq

Optimization of Transesterification Parameters of Biodiesel Produced from Sword Bean (Cavalia gladiate) Seed Oil

The subject of this study was to optimize the transesterification parameters of biodiesel produced from sword bean (C. gladiate) using Response Surface Methodology (RSM). Four transesterification parameters (Reaction temperature 55 0C, Catalyst concentration 0.4 wt%, Methanol to oil ratio 4:1 and reaction time 60 min) were optimized. Data obtained revealed that the optimum reaction conditions for the transesterification of C. gladiata seed oil resulted in 92.88% biodiesel yield. Multiple regression analysis produced a quadratic polynomial equation for methylester and a linear relationship was recorded between the observed and predicted value (R2 = 0.9647) with a significant molar ratio and temperature.  The interaction terms of methanol to oil, molar ratio and temperature with reaction time exhibited a positive effect on the methylester yield. The response surface methodology was found to be a suitable technique for optimizing transesterification process and producing biodiesel that meets the ASTM standard

Study of candidate Be stars in the Magellanic Clouds using near-infrared photometry and optical spectroscopy

Mennickent et al. and Sabogal et al. identified a large number of classical Be (CBe) candidates (∼3500) in the Large and Small Magellanic Clouds (LMC and SMC) based on their photometric variability using the OGLE II data base. They classified these stars into four different groups based on the appearance of their variability. In order to refine and understand the nature of this large number of stars, we studied the infrared properties of the sample and the spectroscopic properties of a subsample. We cross‐correlated the optical sample with the IRSF‐MCPS catalogue to obtain the J, H, Ks magnitudes of all the four types of stars (∼2500) in the LMC and SMC. Spectra of 120 stars belonging to the types 1, 2 and 3 were analysed to study their spectral properties. Among the four types, the type 4 stars are the dominant group, with ∼60 and ∼65 per cent of the total sample in the LMC and SMC, respectively. The near‐infrared (NIR) colour–colour diagrams suggest that the type 4 stars in the LMC have a subclass, which is not found in our Galaxy or in the SMC. This subclass is ∼18 per cent of the type 4 sample. The main type 4 sample which is ∼49 per cent of the total sample has NIR properties similar to the Galactic CBe stars and the SMC type 4 stars. Though the new subclass of type 4 stars have high E(B−V) ∼ 0.75, they are not located close to regions with high reddening. The type 3 stars (∼6 per cent and 7.3 per cent in the LMC and SMC) are found to have large Hα equivalent width (EW) in the SMC and some are found to have large NIR excess. This small fraction of stars are unlikely to be CBe stars. Three stars among the type 3 stars in the LMC are found to be double periodic variables. The type 2 stars are found in larger fraction in the SMC (∼14.5 per cent), when compared to the LMC (∼6 per cent). The spectroscopic and the NIR properties suggest that these could be CBe stars. The type 1 stars are relatively more in the LMC (∼24 per cent) when compared to the SMC (∼13 per cent). The SMC type 1 stars have relatively large Hα EW and this class has properties similar to CBe stars. The spectroscopic sample of type 1 stars which show Hα in emission and are confirmed as CBe stars are more abundant in the SMC by a factor of 2.6. If the effect of metallicity is to cause more CBe stars in the SMC, when compared to the LMC, then type 1, type 2 and type 4 stars follow this rule, with an enhancement of 2.6, 2.4 and 1.3, respectively

Buccal Drug Delivery: Past, Present and Future – A Review

The major hindrance for the absorption of a drug taken orally is extensive first pass metabolism or stability problems within the GI environment like instability in gastric pH and complexation with mucosal membrane. These obstacles can be overcome by altering the route of administration as parenteral, transdermal or trasmucosal. Among these trasmucosal has the advantage of ease of administration, patient compliance and are economic too. The mucosa of the buccal cavity is the most easily accessible transmuocosal site. Buccal transmucosal delivery helps to bypass first- pass metabolism by allowing direct access to the systemic circulation through the internal jugular vein. The buccal transmucosal route has been researched for a wide variety of drugs. Several methodologies have been considered so far, to design and manipulate the release properties towards the invention of buccal mucosal delivery systems. This article aims at reviewing the numerous techniques that has been designed till date for optimizing buccal transmucosal drug delivery.Keywords: complexation; parenteral; transdermal; trasmucosa