26 research outputs found
Screening Target Specificity of siRNAs by Rapid Amplification of cDNA Ends (RACE) for Non-Sequenced Species
Full text linkRNA interference (RNAi) is the process of sequence-specific posttranslational gene silencing triggered by double-stranded RNAs (dsRNAs). RNAi is a widely used approach for studying gene function. However, studies have shown that using siRNA can lead to off-target effects when the siRNA contains sufficient sequence identity to non-target mRNA sequences. One of the important steps in designing dsRNA is verification that it has sequence identity to only the target mRNA. In this report, we propose an approach for primary screening dsRNAs for potential off-target effects by using rapid amplification of cDNA ends. This method can be especially useful for model systems using species that have limited availability of sequence data
Changes in expansin gene expression, IAA content, and extension growth of leaf cells in maize plants subjected to salinity
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Activation of mammalian Tolloid-like 1 expression by hypoxia in human neuroblastoma SH-SY5Y cells
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Inhibition of microRNA-711 limits angiopoietin-1 and Akt changes, tissue damage, and motor dysfunction after contusive spinal cord injury in mice
No full textIdentification of Circular RNA Expression Profiles and their Implication in Spinal Cord Injury Rats at the Immediate Phase
No full textCloning and Characterization of Glutamate Receptor Subunit 4 (GLUA4) and its Alternatively Spliced Isoforms in Turtle Brain
No full textRutin maintains redox balance to relieve oxidative stress induced by TBHP in nucleus pulposus cells
No full textLithium alleviated spinal cord injury (SCI)-induced apoptosis and inflammation in rats via BDNF-AS/miR-9-5p axis
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