Vaccine efficacy in swine challenged with a highly virulent S. typhimurium

Vaccination is one of several options to reduce the impact of Salmonella infections in pork. To demonstrate cross protection against infection with S. typhimurium, pigs were vaccinated with a live vaccine (Enterisol SC-54, Boehringer Ingelheim Vetmedica, Inc.), left as positive challenge controls or negative, non challenged controls

Salmonella Surveillance in U.S. Swine Herds

Past research has focused on Salmonella choleraesuis virulence determinants and the interaction of this organism with the porcine immune system. Emphasis was placed on interaction of virulent S. choleraesuis field isolates with neutrophils and macrophages. Results suggested a correlation between relative bacterial virulence and the organisms ability to interfere with phagocyte ingestion, inhibit superoxide anion production, and overall intracellular survivability. A 40 kDal outer membrane protein was identified and determined to be involved with bacterial translocation and eukaryotic cell invasion

A hard metallic material: Osmium Diboride

We calculate the structural and electronic properties of OsB2 using density functional theory with or without taking into account spin-orbit (SO) interaction. Our results show that the bulk modulus with and without SO interaction are 364 and 365 Gpa respectively, both are in good agreement with experiment (365-395 Gpa). The evidence of covalent bonding of Os-B, which plays an important role to form a hard material, is indicated both in charge density, atoms in molecules analysis, and density of states analysis. The good metallicity and hardness of OsB2 might suggest its potential application as hard conductors.Comment: Figures improve

Age-dependent resistance to Porcine reproductive and respiratory syndrome virus replication in swine

<p>Abstract</p> <p>Background</p> <p>Porcine reproductive and respiratory syndrome virus (PRRSV) causes a prolonged, economically devastating infection in pigs, and immune resistance to infection appears variable. Since the porcine adaptive immune system is not fully competent at birth, we hypothesized that age influences the dynamics of PRRSV infection. Thus, young piglets, growing 16-20-week-old finisher pigs, and mature third parity sows were infected with virulent or attenuated PRRSV, and the dynamics of viral infection, disease, and immune response were monitored over time.</p> <p>Results</p> <p>Virulent PRRSV infection and disease were markedly more severe and prolonged in young piglets than in finishers or sows. Attenuated PRRSV in piglets also produced a prolonged viremia that was delayed and reduced in magnitude, and in finishers and sows, about half the animals showed no viremia. Despite marked differences in infection, antibody responses were observed in all animals irrespective of age, with older pigs tending to seroconvert sooner and achieve higher antibody levels than 3-week-old animals. Interferon γ (IFN γ) secreting peripheral blood mononuclear cells were more abundant in sows but not specifically increased by PRRSV infection in any age group, and interleukin-10 (IL-10) levels in blood were not correlated with PRRSV infection status.</p> <p>Conclusion</p> <p>These findings show that animal age, perhaps due to increased innate immune resistance, strongly influences the outcome of acute PRRSV infection, whereas an antibody response is triggered at a low threshold of infection that is independent of age. Prolonged infection was not due to IL-10-mediated immunosuppression, and PRRSV did not elicit a specific IFN γ response, especially in non-adult animals. Equivalent antibody responses were elicited in response to virulent and attenuated viruses, indicating that the antigenic mass necessary for an immune response is produced at a low level of infection, and is not predicted by viremic status. Thus, viral replication was occurring in lung or lymphoid tissues even though viremia was not always observed.</p

How can project-based mentorships enhance the dietetics profession?

Research Outcomes: The RD Mentorship Program is designed to provide dietetic students (mentee) with the opportunity to gain one-on-one experience with a Registered Dietitian (mentor). This nationwide program evaluates project-based mentorships. Methods: Potential mentors and mentees completed an initial survey that indicated mentor projects and mentee interests. Mentees (n=378) were matched to a mentor (n=264) based on their project preferences and interests. Matches met virtually monthly from September 2020 to April 2021. A mid-program evaluation was sent out in December 2020. Analysis: Descriptive statistics were used to analyze the data Results: The mid-program evaluation indicated that 96% (n=265) of participants found the application and matching process good to very good. The majority of mentee participants liked the variety of dietitians and projects. Some of the projects were, but not limited to, the following: social media and website development, food photography, grocery store tours, virtually shadowing, recipe development, community involvement projects, meal plans, writing and research, analyzing recipes for allergens, literature reviews, cooking classes, case studies, creating exams, resume building, newsletters, course development, interviewing skills development, public policy task force, community assessments, reviewing needs assessments, menu reviews and development, podcast development and patient education handouts. Conclusion: Through the RD Mentorship Program’s project-based mentorship format, mentees are exposed to a broad range of activities that Registered Dietitians perform across many sectors. These projects help to build relationships and offer valuable experiential learning opportunities in dietetics

Ferromagnetism in the RhGd and PtGd systems

Rhodium and platinum have been studied with up to 33% additions of gadolinium. The magnetic properties of the constituents in the layers of a eutectic structure are different from the bulk properties of the constituents. The compound GdPt5 orders ferromagnetically at 13.9 K

Comparison of outcomes of salvage robot-assisted laparoscopic prostatectomy for post-primary radiation vs focal therapy

Objectives: To compare salvage robot-assisted laparoscopic prostatectomy (RALP) outcomes in patients who underwent radiation and those who underwent focal ablation as primary therapies. Patients and Methods: We evaluated 126 patients who underwent salvage RALPbetween 2008 and 2018. Of these, 94 (74.6%) received radiation and 32 focal ablation (25.4%) as primary therapy. These groups were compared with regard to clinical, oncological and functional outcomes. Kaplan–Meier curves and regression models were used to identify survival estimations and their predictors. Results: Before surgery, more patients were potent in the focal ablation group compared to the radiation group (46.9% vs 22.6%; P = 0.013). Peri-operative characteristics and complication rates were not significantly different between the two groups. Postoperative catheterization duration was shorter in the focal ablation group (mean 10 vs 16&nbsp;days; P = 0.018). At final pathology, the focal ablation group had higher non-organ-confined disease (71% vs 50%; P = 0.042) and positive surgical margin (PSM) rates (43.8% vs 17%; P = 0.004) as compared to the radiation group; however, 5-year biochemical recurrence (BCR)-free survival rates were similar (59% vs 56%; P = 0.761). Postoperative 1-year full (no pads/day) and social (0–1&nbsp;pad/day) continence rates were significantly higher in the focal ablation as compared to the radiation group (77.3% vs 39.2%, P = 0.002, and 87.5% vs 51.3%, P = 0.002, respectively). Multivariate analyses showed primary focal ablation and nerve-sparing to be predictors of postoperative continence. Erectile function was preserved in 13% and 27% of preoperatively potent patients in the radiation and focal ablation groups, respectively (P = 0.435). No predictors were identified for postoperative potency. Conclusions: Radiation was associated with inferior functional outcomes after salvage RALP. Focal therapies were associated with higher non-organ-confined disease and PSMrates, with no significant difference in short-term BCR-free survival

Trends in clinical and oncological outcomes of robot-assisted radical prostatectomy before and after the 2012 US Preventive Services Task Force recommendation against PSA screening: a decade of experience

Objective: To assess the influence of the 2012 US Preventive Services Task Force (USPSTF) recommendation against prostate-specific antigen (PSA)-based screening on oncological and functional outcomes following robot-assisted laparoscopic prostatectomy (RALP). Materials and Methods: We retrospectively analysed patients who underwent RALP between 2008 and 2018 with a minimum of 12-month follow-up from a prospectively collected institutional review board-approved database. The impact of the USPSTF recommendation against PSA screening on our surgical outcomes was assessed using a logistic regression model using two groups comprising patients treated before/after the USPSTF statement and indicating time trends for each successive year. Results: The mean preoperative PSA increased from 6.0 to 7.4&nbsp;ng/mL after the USPSTF recommendation. We detected statistically significant time-trend changes after 2012, including an increase in the positive slope of Gleason ≥3 + 4 or ≥pT3 disease. We detected a fall in bilateral full nerve-sparing and an increase in partial nerve-sparing. The total positive surgical margin (PSM) rate increased after the USPSTF recommendation; however, PSM rates pertinent to each pathological stage did not change significantly after 2012. There was a significant negative trend change in the postoperative 12-month continence and potency rates, indicating a breakpoint in functional outcomes after 2012. We detected a 1.7-fold increase in 12-month biochemical recurrence (BCR) rates. The 12-month BCR, potency and continence rates were maintained in young (&lt;55&nbsp;years) patients with a Sexual Health Inventory for Men score &gt;22 and low-volume disease. Conclusion: Since the USPSTF’s recommendation in 2012, we have seen a significant increase in the incidence of high-risk disease that has forced us to modify our approach to the procedure and the grade of nerve-sparing used, leading to a wider resection, in order to reduce PSMs. This has led to a decrease in postoperative functional recovery. Patients with favourable characteristics had good outcomes before and after the USPSTF’s recommendation, implying that the quality of surgery did not change over time

Correlation of salmonella spp. In pigs at slaugther as determined by bacterial culture and salmonella ELISA

The purpose of this study was to detennine the prevalence of Salmonella spp. in pigs at slaughter by bacterial culture, and serology using the Danish Mix-ELISA. Fecal samples and mesenteric lymph nodes were collected from 20 farms (30-35 samples per farm) and cultured for Salmonella on XLD agar following a pre-enrichment in buffered peptone water at 37° C for 24 hours and enrichment in Rappaport-Vassiliadis broth at 42° C for 24 hours. Eighteen of 20 farms yielded at least one positive sample by culture with farm sample prevalence ranging from 3.3% to 96.6%. There were 16 different serotypes of Salmonella isolated. Individual farms had from 0 - 6 different serotypes detected by bacteriologic examination. The Danish Mix-ELISA, developed by Nielsen et al. and currently used in the Danish slaughter plants, was used to test sera collected at slaughter. Using the Mix-ELISA, 16/20 farms were positive with farm sample prevalence ranging from 28.6% to 100%. In comparing the farms, there was a direct correlation between lymph node culture and MixELISA detection levels. These data suggest that the Mix-ELISA may be a valuable herd screening tool for the evaluation of Salmonella levels in U.S. swine herds. Also, these data suggest that vaccination of pigs with SC-54 significantly reduces the prevalence of Salmonella when measured by serology, mesenteric lymph node culture, and fecal culture

COVID-19 model-based practice changes in managing a large prostate cancer practice: following the trends during a month-long ordeal

Coronavirus (COVID-19) has been a life-changing experience for both individuals and institutions. We describe changes in our practice based on real-time assessment of various national and international trends of COVID-19 and its effectiveness in the management of our resources. Initial risk assessment and peak resource requirement using the COVID-19 Hospital Impact Model for Epidemics (CHIME) and McKinsey models. Strengths, weaknesses, opportunities, and threats (SWOT) analysis of our practice\u2019s approach during the pandemic. Based on CHIME the community followed 60% social distancing, the number of expected new patients hospitalized at maximum surge would be 401, with 100 patients requiring ventilator support. In contrast, when the community followed 15% social distancing, the maximum surge of hospitalized new patients would be 1823 and 455 patients would require a ventilator. on April 15, the expected May requirement of ICU beds at peak would be 68, with 61 patients needing ventilators. The estimated surge numbers improved throughout April, and on April 22 the expected ICU bed peak in May would be 11.7, and those requiring ventilator would be 10.5. Simultaneously, within a month, our surgical waitlist grew from 585 to over 723 patients. Our SWOT analysis revealed our internal strengths and inherent weakness, relevant to the pandemic. A graded and a guarded response to this type of situation is crucial in managing patients in a large practice