A Myelin Proteolipid Protein-LacZ Fusion Protein Is Developmentally Regulated and Targeted to the Myelin Membrane in Transgenic Mice

Transgenic mice were generated with a fusion gene carrying a portion of the murine myelin proteolipid protein (PLP) gene, including the first intron, fused to the E. coli LacZ gene. Three transgenic lines were derived and all lines expressed the transgene in central nervous system white matter as measured by a histochemical assay for the detection of β-galactosidase activity. PLP-LacZ transgene expression was regulated in both a spatial and temporal manner, consistent with endogenous PLP expression. Moreover, the transgene was expressed specifically in oligodendrocytes from primary mixed glial cultures prepared from transgenic mouse brains and appeared to be developmentally regulated in vitro as well. Transgene expression occurred in embryos, presumably in pre- or nonmyelinating cells, rather extensively throughout the peripheral nervous system and within very discrete regions of the central nervous system. Surprisingly, beta-galactosidase activity was localized predominantly in the myelin in these transgenic animals, suggesting that the NH_2-terminal 13 amino acids of PLP, which were present in the PLP-LacZ gene product, were sufficient to target the protein to the myelin membrane. Thus, the first half of the PLP gene contains sequences sufficient to direct both spatial and temporal gene regulation and to encode amino acids important in targeting the protein to the myelin membrane

Polarization Observations with the Cosmic Background Imager

We describe polarization observations of the CMBR with the Cosmic Background Imager, a 13 element interferometer which operates in the 26-36 GHz band from Llano de Chajnantour in northern Chile. The array consists of 90-cm Cassegrain antennas mounted on a steerable platform which can be rotated about the optical axis to facilitate polarization observations. The CBI employs single mode circularly polarized receivers which sample multipoles from ℓ~400 to ℓ~4250. The instrumental polarization of the CBI was calibrated with 3C279, a bright polarized point source which was monitored with the VLA

Radio to gamma-ray variability study of blazar S5 0716+714

We present the results of a series of radio, optical, X-ray, and γ-ray observations of the BL Lac object S50716+714 carried out between April 2007 and January 2011. The multifrequency observations were obtained using several ground- and space-based facilities. The intense optical monitoring of the source reveals faster repetitive variations superimposed on a long-term variability trend on a time scale of ~350 days. Episodes of fast variability recur on time scales of ~60−70 days. The intense and simultaneous activity at optical and γ-ray frequencies favors the synchrotron self-Compton mechanism for the production of the high-energy emission. Two major low-peaking radio flares were observed during this high optical/γ-ray activity period. The radio flares are characterized by a rising and a decaying stage and agrees with the formation of a shock and its evolution. We found that the evolution of the radio flares requires a geometrical variation in addition to intrinsic variations of the source. Different estimates yield robust and self-consistent lower limits of δ ≥ 20 and equipartition magnetic field B_eq ≥ 0.36 G. Causality arguments constrain the size of emission region θ ≤ 0.004 mas. We found a significant correlation between flux variations at radio frequencies with those at optical and γ-rays. Theoptical/GeV flux variations lead the radio variability by ~65 days. The longer time delays between low-peaking radio outbursts and optical flares imply that optical flares are the precursors of radio ones. An orphan X-ray flare challenges the simple, one-zone emission models, rendering them too simple. Here we also describe the spectral energy distribution modeling of the source from simultaneous data taken through different activity periods

A Multi-wavelength Study of the Host Environment of SMBHB 4C+37.11

4C+37.11, at z=0.055 shows two compact radio nuclei, imaged by VLBI at 7mas separation, making it the closest known resolved super-massive black hole binary (SMBHB). An important question is whether this unique object is young, caught on the way to a gravitational in-spiral and merger, or has `stalled' at 7pc. We describe new radio/optical/X-ray observations of the massive host and its surrounding X-ray halo. These data reveal X-ray/optical channels following the radio outflow and large scale edges in the X-ray halo. These structures are promising targets for further study which should elucidate their relationship to the unique SMBHB core.Comment: To appear in the Astrophysical Journa

Lobe Advance Velocities in the Extragalactic Compact Symmetric Object 4C 31.04

We report on the results of a two epoch study of the low power Compact Symmetric Object 4C 31.04. Observations performed with the VLBA at 5 GHz in 1995 and 2000 have yielded images of this source at milliarcsecond angular resolution. A central core is detected, with bright compact hot spots and extended lobes on both sides. Model-fitting and other analysis of the data (brightness profile, difference map) clearly indicate that the source is expanding. We estimate the velocity of this expansion to be (0.085 +/- 0.016) mas/yr, i.e. (0.33 +/- 0.06) c in both hot spots. Assuming a constant expansion velocity, we estimate the kinematic age of the source at 550 yrs. We also study the spectral index using VLBA observations at 1.3 GHz and MERLIN at 22 GHz. The derived spectral age is 3000-5000 years in equipartition conditions. The two estimates are discussed and found to be in agreement, given present uncertainties.Comment: 9 pages, 8 .eps figures; accepted for publication in Astronomy & Astrophysic

Contemporaneous VLBA 5 GHz Observations of Large Area Telescope Detected Blazars

The radio properties of blazars detected by the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope have been observed contemporaneously by the Very Long Baseline Array (VLBA). In total, 232 sources were observed with the VLBA. Ninety sources that were previously observed as part of the VLBA Imaging and Polarimetry Survey (VIPS) have been included in the sample, as well as 142 sources not found in VIPS. This very large, 5 GHz flux-limited sample of active galactic nuclei (AGNs) provides insights into the mechanism that produces strong γ-ray emission. In particular, we see that γ-ray emission is related to strong, uniform magnetic fields in the cores of the host AGN. Included in this sample are non-blazar AGNs such as 3C84, M82, and NGC 6251. For the blazars, the total VLBA radio flux density at 5 GHz correlates strongly with γ-ray flux. The LAT BL Lac objects tend to be similar to the non-LAT BL Lac objects, but the LAT flat-spectrum radio quasars (FSRQs) are significantly different from the non-LAT FSRQs. Strong core polarization is significantly more common among the LAT sources, and core fractional polarization appears to increase during LAT detection

The properties of the gamma-ray blazars in the CJ-F VLBI sample

We present first results from the analysis of multi-epoch VLBI observations of the EGRET detected sources [9] in the CJ-F sample (Caltech Jodrell-Flat-spectrum, [10]). These objects form a subsample of 14 sources within the 293 AGN of the full CJ-F sample. 5 GHz VLBI snapshot observations of the CJ-F sources are continuously being performed in order to create a valid database for thorough statistical tests of pc-scale jet motion in AGN. All gamma-bright CJ-F AGN have been observed at least twice with the VLBA, which enables us to investigate jet component motions and paths. In particular, we concentrate on the analysis of those properties supposed to be essential for gamma-ray production, i.e., superluminal motion and bending. A paper discussing the possible relation between morphological changes and gamma-ray flaring/production is in preparation

Characteristics of Gamma-Ray Loud Blazars in the VLBA Imaging and Polarimetry Survey

The radio properties of blazars detected by the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope have been observed as part of the VLBA Imaging and Polarimetry Survey (VIPS). This large, flux-limited sample of active galactic nuclei (AGN) provides insights into the mechanism that produces strong gamma-ray emission. At lower flux levels, radio flux density does not directly correlate with gamma-ray flux. We find that the LAT-detected BL Lacs tend to be similar to the non-LAT BL Lacs, but that the LAT-detected FSRQs are often significantly different from the non-LAT FSRQs. The differences between the gamma-ray loud and quiet FSRQs can be explained by Doppler boosting; these objects appear to require larger Doppler factors than those of the BL Lacs. It is possible that the gamma-ray loud FSRQs are fundamentally different from the gamma-ray quiet FSRQs. Strong polarization at the base of the jet appears to be a signature for gamma-ray loud AGN.Comment: 32 pages, 9 figures, accepted by Ap