Bitter taste receptors expression in human granulosa and cumulus cells: New perspectives in female fertility

Bitter taste receptors (TAS2RS) expression is not restricted to the oral cavity and the presence of these receptors in the male reproductive system and sperm provides insights into their possible role in human reproduction. To elucidate the potential role of TAS2Rs in the female reproductive system, we investigated the expression and localization of bitter taste receptors and the components of signal transduction cascade involved in the pathway of taste receptors in somatic follicular cells obtained from women undergoing assisted reproductive techniques. We found that TAS2R genes are expressed in both cumulus (CCs) and granulosa (GCs) cells, with TAS2R14 being the most highly expressed bitter receptor subtype. Interestingly, a slight increase in the expression of TAS2R14 and TAS2R43 was shown in both GCs and CCs in young women (p < 0.05), while a negative correlation may be established between the number of oocytes collected at the pickup and the expression of TAS2R43. Regarding α-gustducin and α-transducin, two Gα subunits expressed in the taste buds on the tongue, we provide evidence for their expression in CCs and GCs, with α-gustducin showing two additional isoforms in GCs. Finally, we shed light on the possible downstream transduction pathway initiated by taste receptor activation in the female reproductive system. Our study, showing for the first time the expression of taste receptors in the somatic ovarian follicle cells, significantly extends the current knowledge of the biological role of TAS2Rs for human female fertility

An XRPD and EPR spectroscopy study of microcrystalline calcite bioprecipitated by Bacillus subtilis

We report in this study the first XRPD and EPR spectroscopy characterisation of a biogenic calcite, obtained from the activity of the bacterium Bacillus subtilis. Microcrystalline calcite powders obtained from bacterial culture in a suitable precipitation liquid medium were analysed without further manipulation. Both techniques reveal unusual parameters, closely related to the biological source of the mineral, i.e., to the bioprecipitation process and in particular to the organic matrix observed inside calcite. In detail, XRPD analysis revealed that bacterial calcite has slightly higher c/a lattice parameters ratio than abiotic calcite. This correlation was already noticed in microcrystalline calcite samples grown by bio-mineralisation processes, but it had never been previously verified for bacterial biocalcites. EPR spectroscopy evidenced an anomalously large value of W6, a parameter that can be linked to occupation by different chemical species in the next nearest neighbouring sites. This parameter allows to clearly distinguish bacterial and abiotic calcite. This latter achievement was obtained after having reduced the parameters space into an unbiased Euclidean one, through an isometric log-ratio transformation. We conclude that this approach enables the coupled use of XRPD and EPR for identifying the traces of bacterial activity in fossil carbonate deposits

The Smart City and the Creation of Local Public Value

none1The distinctive feature of local authorities is the creation of public value in a financially sustainable way. In other words, they are expected to meet effectively the public needs of citizens, generating a positive spread between social benefits and costs and thus contributing to the prosperity of their community of reference (i.e. their constituency). At the same time, they are expected to pursue financial stability by efficiently using the increasingly scarce and therefore precious public resources. The fulfillment of this function is very complex, due to both the growing proliferation of public needs and the progressive lack of available resources, but also because it is significantly affected by the roles played by many other actors, including citizens, businesses, other public authorities and not-for-profit organizations. To address this difficulty, many local authorities state, in their strategic plans, that they wish to become smart. A smart city, in fact, identifies an urban environment actively engaged in improving the quality of life of its citizens and in pursuing sustainable socio-economic development, thanks to the wide and innovative use of information and communication technology. However, so far the concept of smart city, although widely used, does not have a consistent meaning and therefore needs to be deepened and better defined. More precisely, this paper seeks to identify fields of action in which the city can be smart, analyzing the potential benefits to quality of life, environmental protection and economic development, but also looking into the possible obstacles and potential solutions in the relationship between the local authority and other actors in the social system. Moreover, even the application of the smart city model is quite varied. In this regard, the paper aims to describe the state of the art of Italian regional capitals, seen as a significant sample of large and medium-size cities in the country, analyze their common and different features, strengths and weaknesses, and suggest some solutions to overcome weaknesses and exploit strengths. Therefore, the aim of this paper is two-fold. On a theoretical level, it aims to contribute to the smart city definition and critically analyze the relationship between this concept and the creation of local public value. On a practical level, it intends to verify the adoption of the smart city model by a significant sample of large and medium-size Italian cities, in order to draw useful indications of perspective. In essence, the paper aims to provide a critical and empirically informed analysis of the potential success, but also possible failure of the smart city projects.Federico FontanaFontana, Federic

Composition of supralittoral sediments bacterial communities in a Mediterranean island

Marine coasts represent highly dynamic ecosystems, with sandy beaches being one of the most heterogeneous. Despite the key importance of sandy beaches as transition ecosystems between sea and land, very few studies on the microbiological composition of beach sediments have been performed. To provide a first description of microbial composition of supralittoral sediments, we investigated the composition of bacterial communities of three sandy beaches, at Favignana Island, Italy, using metagenetic approaches (Terminal-Restriction Fragment Length Polymorphism, sequencing of 16S rRNA genes by Illumina-Solexa technology, functional genes detection, and quantitative Real-Time PCR). Results showed that the investigated beaches are harboring a rich bacterial diversity, mainly composed by members of classes Alphaproteobacteria, Gammaproteobacteria, Flavobacteria and Actinobacteria. The metagenetic analysis showed profiles of decreasing beta diversity and increasing richness, as well as a differentiation of communities, along the sea-to-land axis. In particular, members of Firmicutes and Proteobacteria displayed contrasting profiles of relative abundance (to decrease and to increase, respectively) along the sea-to-land axis of the beach. Finally, a search for the presence of genes related to the nitrogen and carbon biogeochemical cycle (nifH, nosZ, pmoA/amoA) detected the presence of ammonia monoxygenase sequences (amoA) only, suggesting the presence of bacterial ammonia oxidation to some extent, probably due to members of Nitrospira, but with the lack of nitrogen fixation and denitrification