Molecular and cellular mechanisms underlying the evolution of form and function in the amniote jaw.

The amniote jaw complex is a remarkable amalgamation of derivatives from distinct embryonic cell lineages. During development, the cells in these lineages experience concerted movements, migrations, and signaling interactions that take them from their initial origins to their final destinations and imbue their derivatives with aspects of form including their axial orientation, anatomical identity, size, and shape. Perturbations along the way can produce defects and disease, but also generate the variation necessary for jaw evolution and adaptation. We focus on molecular and cellular mechanisms that regulate form in the amniote jaw complex, and that enable structural and functional integration. Special emphasis is placed on the role of cranial neural crest mesenchyme (NCM) during the species-specific patterning of bone, cartilage, tendon, muscle, and other jaw tissues. We also address the effects of biomechanical forces during jaw development and discuss ways in which certain molecular and cellular responses add adaptive and evolutionary plasticity to jaw morphology. Overall, we highlight how variation in molecular and cellular programs can promote the phenomenal diversity and functional morphology achieved during amniote jaw evolution or lead to the range of jaw defects and disease that affect the human condition

Flea-borne rickettsioses: ecologic considerations.

Ecologic and economic factors, as well as changes in human behavior, have resulted in the emergence of new and the reemergence of existing but forgotten infectious diseases during the past 20 years. Flea-borne disease organisms (e.g., Yersinia pestis, Rickettsia typhi, R. felis, and Bartonella henselae) are widely distributed throughout the world in endemic-disease foci, where components of the enzootic cycle are present. However, flea-borne diseases could reemerge in epidemic form because of changes in vector-host ecology due to environmental and human behavior modification. The changing ecology of murine typhus in southern California and Texas over the past 30 years is a good example of urban and suburban expansion affecting infectious disease outbreaks. In these areas, the classic rat-flea-rat cycle of R. typhi has been replaced by a peridomestic animal cycle involving, e.g., free-ranging cats, dogs, and opossums and their fleas. In addition to the vector-host components of the murine typhus cycle, we have uncovered a second typhuslike rickettsia, R. felis. This agent was identified from the blood of a hospitalized febrile patient and from opossums and their fleas. We reviewed the ecology of R. typhi and R. felis and present recent data relevant to the vector biology, immunology, and molecular characterization and phylogeny of flea-borne rickettsioses

Knowledge and practices of residents in two north Namibian towns with regard to rabies and pet care

Recent studies have highlighted the epidemiology of rabies in southern Africa and the unique nature of kudu rabies in Namibia. However, the serious effect on human populations in northern Namibia lacks focused attention. This study surveyed knowledge and awareness of rabies, including its prevention and pet care in two towns in the Oshana Region of Namibia. Of the 245 interviewed, two thirds owned at least one dog, while a third owned a cat. Eighty-one per cent allowed their animals to roam freely, while 14% reported having been bitten by a dog. The majority of those surveyed recognised that rabies is caused by a virus (53%), identified a dog bite as the main means of transmission (90.6%), cited wild animals as reservoirs (75.5%) and knew that dogs and jackals are significant reservoirs (96.3%). Only 35 (14.3%) identified the correct answers to all four questions. Most of the study participants (63.3%) received their information about rabies from the media. While 83% knew that free vaccines are available at government offices, only 37% had vaccinated their pets, and only 6% had been vaccinated themselves. The results indicate a general understanding of rabies, but focused education efforts are needed with respect to community members in order for specific points to be clarified. The high level of knowledge of vaccine availability, but low coverage, indicates the need for mobilisation with regard to at-risk populations. By building on what is already known, future programmes should successfully reach populations throughout northern Namibia and control rabies in the future.Peer reviewedEntomology and Plant Patholog

Olfactory responses of Amblyomma maculatum to rumen fluid and other odourants that attract blood-seeking arthropods

Amblyomma maculatum Koch (Ixodida: Ixodidae) has emerged as a significant vector of human and companion animal diseases in the U.S.A. When expanding in range, A. maculatum can be difficult to collect in the field and control on livestock. A novel method is needed to improve the field collection of A. maculatum, as well as to control their effects as ectoparasites of livestock and companion animals. The present study aimed to test the effects of known volatiles on the activation and selection choices of A. maculatum in a laboratory-based Y-tube assay and field-based assays. Although the majority of adult A. maculatum were activated to move by five of the seven semiochemicals tested, only rumen fluid significantly attracted ticks to make a selection in the Y-tube apparatus. Rumen fluid attracted the most A. maculatum in the laboratory, with 56% (84/150) making it to the rumen Y-tube arm, although the results were not replicated in semi-field experiments. These studies highlight the need for continued work to identify attractants for tick vectors that will assist field collections. These attractants could also be incorporated into management strategies that lead to prevention technologies to reduce tick burdens on cattle or in risk areas of humans.Peer reviewedEntomology and Plant Patholog

Morphology and foraging behavior of Oklahoma's grass-feeding termite: Gnathamitermes tubiformans (Isoptera: Termitidae)

Grass-feeding termites, Gnathamitermes sp., can be considered beneficial in pastureland, where they aerate topsoil, recycle nutrients from grasses and forbs, and excavate and redistribute soil. The primary objective of this report is to illustrate and determine the average physical dimensions of Gnathamitermes tubiformans soldiers by measuring key body parts, including left mandible length, as well as discuss observed foraging behavior. G.Tubiformans soldiers have unique mandibles that can be used for accurate identification. Workers are morphologically similar compared with some other arid-land termite species, thus soldier characteristics are used for identification. The secondary objective is to describe observations of G.Tubiformans foraging behavior. This report also provides detailed line drawings and a watercolor illustration for aid in identification. Termites were collected near Temple, Cotton County, Oklahoma, and measured with high precision microscopy. To ensure accurate identification, fourteen different soldier body measurements were determined. The ability to identify termites that are potentially beneficial or detrimental to forage grass biomass production may aid efforts to enhance livestock production on pastures.Peer reviewedEntomology and Plant PathologyPsycholog

Mosquito ingestion of antibodies against mosquito midgut microbiota improves conversion of ookinetes to oocysts for Plasmodium falciparum, but not P. yoelii

The mosquito midgut is a site of complex interactions between the mosquito, the malaria parasite and the resident bacterial flora. In laboratory experiments, we observed significant enhancement of Plasmodium falciparum oocyst production when Anopheles gambiae (Diptera: Culicidae) mosquitoes were membrane-fed on infected blood containing gametocytes from in vitro cultures mixed with sera from rabbits immunized with A. gambiae midguts. To identify specific mechanisms, we evaluated whether the immune sera was interfering with the usual limiting activity of gram-negative bacteria in An. gambiae midguts. Enhancement of P. falciparum infection rates occurred at some stage between the ookinete and oocyst stage and was associated with greater numbers of oocysts in mosquitoes fed on immune sera. The same immune sera did not affect the sporogonic development of P. yoelii, a rodent malaria parasite. Not only did antibodies in the immune sera recognize several types of midgut-derived gram-negative bacteria (Pseudomonas spp. and Cedecea spp.), but gentamicin provided in the sugar meal 3. days before an infectious P. falciparum blood meal mixed with immune sera eliminated the enhancing effect. These results suggest that gram-negative bacteria, which normally impair P. falciparum development between the ookinete and oocyst stage, were altered by specific anti-bacterial antibodies produced by immunizing rabbits with non-antibiotic-treated midgut lysates. Because of the differences in developmental kinetics between human and rodent malaria species, the anti-bacterial antibodies had no effect on P. yoelii because their ookinetes leave the midgut much earlier than P. falciparum and so are not influenced as strongly by resident midgut bacteria. While this study highlights the complex interactions occurring between the parasite, mosquito, and midgut microbiota, the ultimate goal is to determine the influence of midgut microbiota on Plasmodium development in anopheline midguts in malaria endemic settings.Peer reviewedEntomology and Plant Patholog

Does the extent of neck surgery based on preoperative calcitonin level influence survival in medullary thyroid carcinoma: a retrospective tertiary centre experience

Introduction Medullary thyroid carcinoma (MTC) is a rare thyroid cancer arising from the calcitonin-secreting parafollicular cells. Previous studies suggested a preoperative calcitonin level >200ng/l is an indication for prophylactic lateral neck dissection (LND) to remove micrometastases and improve survival outcomes. Methods This retrospective single-centre study assessed the efficacy of preoperative calcitonin levels as a marker for determining need for prophylactic LND in MTC. Data were obtained on demographics, preoperative calcitonin levels, size and laterality of index tumour, type of neck dissection (central neck dissection (CND), LND), nodes removed, levels with involved nodes, number of nodes histologically involved, mortality, adjuvant therapy and locoregional recurrence. Results A total of 33 patients were identified from St George's University Hospitals NHS Foundation Trust between 1 January 2001 and 19 August 2021; 8 were excluded for data quality issues. Of the 18 classified with a high preoperative calcitonin level (>200ng/l), 10 (56%) had a LND and 8 (44%) had a CND. In the low-calcitonin group, three (43%) patients had a CND only and four (57%) had a LND. There was no difference in absolute or disease-free survival between the low and high groups (p=0.960, p=0.817), or between those who had a CND and LND in the high-calcitonin group (p=0.607, hazard ratio (HR) 0.55; p=0.129, HR 8.78). Conclusion There was no statistically significant difference in outcomes between high and low calcitonin groups. A selective approach to performing LND in MTC patients based on clinical and imaging findings suggesting disease presence in the lateral neck should be explored further