METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF CURCUMIN AND PIPERINE BY RP-HPLC

Objective: To develop a simple, sensitive, specific, accurate reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of curcumin and piperine.Methods: The separation was done using a column Inertsil–ODS C18 (250 mm × 4.6 mm, 5µ particle size) and mobile phase composed of methanol: water (45:55 v/v), flow rate at 1 ml/min and detection was carried out at 282 nm with photodiode array (PDA) detector.Results: The separation of curcumin and piperine were found to be at the retention time of 2.433 min and 3.095 min, respectively. The method was found to be linear at a concentration range 20-80 µg/ml for curcumin and piperine. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.05µg/ml and 0.17µg/ml for curcumin and 0.18µg/ml and 0.53µg/ml for piperine respectively. The average percentage recoveries of curcumin and piperine were in the range of 98-100.6%.Conclusion: A simple and sensitive reverse phase high performance liquid chromatographic method was developed for the estimation of curcumin and piperine

A NOVEL STABILITY-INDICATING REVERSE PHASE LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN AND TENELIGLIPTIN IN PURE AND PHARMACEUTICAL FORMULATIONS

Objective: The present method was proposed to develop a simple, sensitive, rapid, accurate and stability-indicating reverse phase-high performance liquid chromatographic (RP-HPLC) method for the simultaneous estimation of metformin and teneligliptin in pure and pharmaceutical formulations. Methods: The chromatographic separation was done on Discovery [250 mm X 4.6 mm: 5 μm is particle size] using a mobile phase composed of 0.1% orthophosphoric acid buffer: acetonitrile [65:35, v/v], the flow rate is 1 ml/min and the detection was carried out with a photodiode array (PDA) at 260 nm.Results: The retention time of metformin and teneligliptin were found to be 2.517 min and 3.687 min, respectively. Stability indicating studies were conducted under the guidelines of an international conference on harmonization [ICH] Q1A R2 and the developed method was validated as per the guidelines of ICH Q2 R1. The linearity was found in the range of concentration of 125-750 μg/ml and 5-30 μg/ml for metformin and teneligliptin. The lower limit of detection (LOD) and lower limit of quantification (LOQ) was found to be 0.02μg/ml and 0.07 μg/ml for metformin and 0.19 μg/ml and 0.56 μg/ml for teneligliptin, respectively.Conclusion: A novel stability-indicating reverse phase liquid chromatographic method developed for the simultaneous estimation of metformin and teneligliptin. The proposed method was adopted for the routine estimation of metformin and teneligliptin in bulk and pharmaceutical dosage forms

A review on natural and synthetic polymers employed in the formulation of oral disintegrating tablets

The oral route is the most popular route of administration for numerous drugs because it is considered as a safest, most preferred and less economical. Disintegrating agents are the substances that disperse or dissolve within a matter of seconds. This disintegrating mechanism helps in dysphagia, pediatric and geriatric patients. Synthetic disintegrating agents are available as a highly economical and less effective when compared to natural disintegrating agents. Therefore natural disintegrants serve as the best alternative to overcome the problems of these synthetic substances. Due to their numerous advantages over synthetic products they are widely used in the pharmaceutical field as a binder, disintegrants, gums, and mucilages. Keywords: Disintegrants, dysphagia, synthetic substances

Cephalopod resources revealed by exploratory surveys in Indian seas

The areawise and depth wise cephalopod catches of fishing vessels of Government of India and some Agencies which conducted exploratory fishing in offshore areas have been presented and discussed. The exploratory fishing by Government of India vessels in Bombay-Gujarat region during 1977-80 yielded a maximum of 7,609 kg of cephalopods a year at a catch rate of 6.8 kg/h. The highest catch rates were recorded from the area 19-70 and the depth zone 80-89 m. In Visakhapatnam region the cephalopod catch was poor during 1968-75 but during 1977-80 the annual catch increased to 3,283 kg. The most intensively fished area was 17-83. The catch was composed of four species of cuttlefishes and three species of squids. The results of trawl surveys in other parts of Indian waters are also dealt with

ANTIPLASMODIAL ACTIVITY OF CAESALPINIA CRISTA SEED EXTRACTS

Objective: To evaluate antiplasmodial activity of Caesalpinia crista seed extracts Methods:  Antiplasmodial activity of the seed extracts of Caesalpinia crista against rodent malaria infections in chloroquine sensitive Plasmodium falcipuram strain was investigated, and oral acute toxicity of seed extracts of Caesalpinia crista was also evaluated. Results: The findings of this study revealed significant (P < 0.05) and dose dependent decrease in parasitaemia in the parasitized groups treated with varying doses of the extract (50-200 mg/kg p.o.) in both suppressive and curative tests. There was also significant decrease in parasitaemia density in the chloroquine treated group. The alcoholic extract was found no toxicity in wistar rats and the oral LD50 was determined to be greater than 5000 mg/kg. Conclusion: Seed extracts of Caesalpinia crista extract possesses potent antiplasmodial activity and may therefore, serve as potential sources of new antimalarial agents Keywords: Plasmodium falcipuram, Caesalpinia crista, Plant extracts, Phytochemicals, Toxicity tests, malaria

Preparation and evaluation of poly herbal hair oil

In Ayurvedic medicine, herbs are used as an integral part of health care system. Besides healthcare, herbs are also used for beautification of the body and for preparation of various cosmetics and colours. The aim of present study involves preparation of poly herbal hair oil using fresh leaves of various plants. The prepared herbal oils were subjected to phytochemical screening, General characterization, Physical and Biological evaluation.The aim of present study involves preparation of poly herbal hair oil using fresh leaves of Sphaeranthus indicus, Wrightia tinctoria, Eclipta alba, Hibiscus Rosa sinensis. The oil was prepared according to Ayurvedic pharmacopeia. The evaluation of prepared poly herbal hair oil was carried out by various parameters such as organoleptic, phytochemical, specific gravity, pH, viscosity, acid value, saponification value, refractive index and stability studies. Antimicrobial activity of the poly herbal hair oil was studied by the zone of inhibition method. The Antioxidant activity of the oil was studied by DPPH radical scavenging test. The prepared formulations are assessed for primary skin irritation test on our forearm. Above parameters were found to be good and within the standards and among three concentrations of prepared poly herbal hair oil, third concentration showed better results than other two concentrations. All the values in the evaluation of finished product showed that they are within the acceptable limits. Hence, it is concluded that the oil is beneficial in maintaining good growth of hairs, turning grey hairs to black, providing protection from dandruff, and results in lustrous looking hairs. Keywords: Hair, Herbal formulations, Hair oil, Stability studies

Cephalopod production in India and constituent maritime states

The fishing craft and gear in which cepbalopods are obtained in India and the all India, statewise and east and west coast cephalopod production are presented. The bulk of the cephalopod catches is obtained along the west coast and trawl net accounts for major portion of landings. Maharashtra, Kerala, Tamil Nadu and Gujarat are the leading states in production

Some aspects of the biology of squids

The results of studies on the biological aspects such as sexuality, sex ratio, size at first maturity, spawning, age and growth, distribution of adults, food, predators,Iife-history and distribution of juveniles of three species of squids Loligo duvaucelii, Sephreuthis lessoniana and Doryteuthis sibogae are presented. The breeding period of all the three species was found to be prolonged. In Lotigo duvaucelii the males attained sexual maturity earlier than females in the various fishing grounds investigated