Whole blood gene expression profiling in preclinical and clinical cattle infected with atypical bovine spongiform encephalopathy

Prion diseases, such as bovine spongiform encephalopathies (BSE), are transmissible neurodegenerative disorders affecting humans and a wide variety of mammals. Variant Creutzfeldt-Jakob disease (vCJD), a prion disease in humans, has been linked to exposure to BSE prions. This classical BSE (cBSE) is now rapidly disappearing as a result of appropriate measures to control animal feeding. Besides cBSE, two atypical forms (named Hand L-type BSE) have recently been described in Europe, Japan, and North America. Here we describe the first wide-spectrum microarray analysis in whole blood of atypical BSEinfected cattle. Transcriptome changes in infected animals were analyzed prior to and after the onset of clinical signs. The microarray analysis revealed gene expression changes in blood prior to the appearance of the clinical signs and during the progression of the disease. A set of 32 differentially expressed genes was found to be in common between clinical and preclinical stages and showed a very similar expression pattern in the two phases. A 22-gene signature showed an oscillating pattern of expression, being differentially expressed in the preclinical stage and then going back to control levels in the symptomatic phase. One gene, SEL1L3, was downregulated during the progression of the disease. Most of the studies performed up to date utilized various tissues, which are not suitable for a rapid analysis of infected animals and patients. Our findings suggest the intriguing possibility to take advantage of whole blood RNA transcriptional profiling for the preclinical identification of prion infection. Further, this study highlighted several pathways, such as immune response and metabolism that may play an important role in peripheral prion pathogenesis. Finally, the gene expression changes identified in the present study may be further investigated as a fingerprint for monitoring the progression of disease and for developing targeted therapeutic interventions. \ua9 2016 Xerxa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Ecosystem-Based Adaptation for the Impact of Climate Change and Variation in the Water Management Sector of Sri Lanka

The climate of Sri Lanka has been fluctuating at an alarming rate during the recent past. These changes are reported to have pronounced impacts on the livelihoods of the people in the country. Water is central to the sustainable functioning of ecosystems and wellbeing of mankind. It is evident that pronounced variations in the climate will negatively impact the availability and the quality of water resources. The ecosystem-based adaptation (EbA) approach has proved to be an effective strategy to address the impact of climate change on water resources in many parts of the world. The key aim of this paper is to elaborate the wide range of benefits received through implementation of EbAs in field level, watershed scale, and urban and coastal environments in the context of Sri Lanka. In addition, this paper discusses the benefits of utilizing EbA solutions over grey infrastructure-based solutions to address the issues related to water management. The wide range of benefits received through implementation of EbAs can be broadly classified into three categories: water supply regulation, water quality regulation, and moderation of extreme events. This paper recommends the utilization of EbAs over grey infrastructure-based solutions in adaptation to climate change in the water management sector for the developing region due its cost effectiveness, ecofriendliness, and multiple benefits received on long-term scales. The findings of this study will unequivocally contribute to filling existing knowledge and research gaps in the context of EbAs to future climate change in Sri Lanka. The suggestions and opinions of this study can be taken into account by decision makers and water resources planning agencies for future planning of actions related to climate change adaptation in Sri Lanka

Projection of Future Hydropower Generation in Samanalawewa Power Plant, Sri Lanka

The projection of future hydropower generation is extremely important for the sustainable development of any country, which utilizes hydropower as one of the major sources of energy to plan the country’s power management system. Hydropower generation, on the other hand, is mostly dependent on the weather and climate dynamics of the local area. In this paper, we aim to study the impact of climate change on the future performance of the Samanalawewa hydropower plant located in Sri Lanka using artificial neural networks (ANNs). ANNs are one of the most effective machine learning tools for examining nonlinear relationships between the variables to understand complex hydrological processes. Validated ANN model is used to project the future power generation from 2020 to 2050 using future projected rainfall data extracted from regional climate models. Results showcased that the forecasted hydropower would increase in significant percentages (7.29% and 10.22%) for the two tested climatic scenarios (RCP4.5 and RCP8.5). Therefore, this analysis showcases the capability of ANN in projecting nonstationary patterns of power generation from hydropower plants. The projected results are of utmost importance to stakeholders to manage reservoir operations while maximizing the productivity of the impounded water and thus, maximizing economic growth as well as social benefits

Laparoscopic Choledochotomy in a Solitary Common Duct Stone: A Prospective Study

Background. Laparoscopic common bile duct exploration has all the advantages of minimal access and is also the most cost effective compared to the other options. Objective. To study a profile on laparoscopic common bile duct exploration for a single common duct stone. Methods. A total of 30 consecutive patients with solitary common bile duct stone attending our hospital over a period of one year were enrolled in the study. Laparoscopic common bile duct exploration was done by transductal route in all the patients. Results. There were 18 females and 12 males with age ranging from 28 to 75 years. Jaundice was present in 12 (40%) patients. Twenty-four (80%) patients had raised alkaline phosphatase. The mean size of CBD on ultrasound was 11.55 mm. The mean size of calculus was 11.06 mm and was located in the distal CBD in 26 (86.7%) patients. The mean operative time was 158.4 ± 57.89 min. There were 8 (26.6%) conversions to open procedure. T-tube was used in 26 (86.7%) patients. The postoperative complications were hospital acquired chest infection in 3 (10%), surgical site infection in 3 (10%), acute coronary syndrome in one (3.3%), and bile leak after T-tube removal in one (3.3%) patient. Conclusions. Laparoscopic common bile duct exploration is an effective, safe management of common bile duct stone

Edge controlled growth of hexagonal boron nitride crystals on copper foil by atmospheric pressure chemical vapor deposition

Most of the chemical vapor deposition (CVD) systems used for hexagonal boron nitride (h-BN) growth employ pyrolytic decomposition of a precursor molecule, such as ammonia borane (AB), at a temperature close to its melting point. So the control of its partial pressure is essential for high quality crystal growth. Here, we report on the edge controlled growth of a h-BN single crystal larger than 25 μm in edge length on purchased Cu foils. The key was the controlled supply of borazine gas generated by the decomposition of AB, and the stepwise decomposition of AB was found to be essential for the growth of regular h-BN crystals. The h-BN growth was mostly governed by the position of the nucleation point rather than Cu orientation as confirmed by electron back-scattered diffraction (EBSD) analysis. It was also demonstrated that the variation in temperature during the growth and cooling processes induced wrinkles larger than 20 nm due to the thermal straining of the Cu surface and a negative expansion coefficient of h-BN. These results provide a detailed understanding of h-BN growth, which will be applicable to other 2D materials

The Efficacy and Safety of Low Dose Epidural Butorphanol on Postoperative Analgesia following Cesarean Delivery

Butorphanol is considered an effective and safe analgesic after cesarean delivery but is associated with profound dose-dependent sedation. Somnolence may cause hindrance in early mother-baby interaction. This study was designed to assess the analgesic efficacy and to monitor side-effects of low doses (0.5 mg and 0.75 mg) of epidural butorphanol with bupivacaine compared to bupivacaine alone in parturients following cesarean delivery. One hundred and twenty parturients (American Society of Anesthesiologists physical status 1 and 2) undergoing cesarean delivery were allocated into three groups: group 1 received epidural 0.125% bupivacaine while group 2 and 3 received an additional 0.5 mg and 0.75 mg butorphanol respectively. A combined spinal, epidural technique was used. Spinal anaesthesia was used for surgery. The epidural route was used for postoperative analgesia with the study drug. Onset, duration and quality of analgesia, lowest visual analogue scales (VAS) score, and side effects were noted. The onset and duration of analgesia in group 2 (4.1±2.6 min and 202.4±62.8 min) and group 3 (4.0±2.5 min and 192.3±69.1 min) were significantly different (P<0.01) from group 1 (6.6±2.7min and145.7±89.6 min). The quality of analgesia in terms of time to first independent movement and satisfactory VAS were statistically better (P<0.01) in group 2 (3.9±0.3 hour and 8.1±0.1 mm) and group 3 (3.8±0.4 hour and 8.1±0.9 mm) than in group 1 (5.2±0.4 hour and 6.3±1.3 mm). The incidence of sedation was 5% in all the three groups. A lower dose of epidural butorphanol with bupivacaine produces a significantly earlier onset, longer duration and better quality of analgesia than bupivacaine does. Key words: analgesia, epidural, postcesarean, spina