Understanding the potential in vitro modes of action of bis(β‐diketonato) oxovanadium(IV) complexes

To understand the potential in vitro modes of action of bis(β-diketonato) oxovanadium(IV) complexes, nine compounds of varying functionality have been screened using a range of biological techniques. The antiproliferative activity against a range of cancerous and normal cell lines has been determined, and show these complexes are particularly sensitive against the lung carcinoma cell line, A549. Annexin V (apoptosis) and Caspase-3/7 assays were studied to confirm these complexes induce programmed cell death. While gel electrophoresis was used to determine DNA cleavage activity and production of reactive oxygen species (ROS), the Comet assay was used to determine induced genomic DNA damage. Additionally, Förster resonance energy transfer (FRET)-based DNA melting and fluorescent intercalation displacement assays have been used to determine the interaction of the complexes with double strand (DS) DNA and to establish preferential DNA base-pair binding (AT versus GC)

A simple and new method for the synthesis of 1,5-benzodiazepine derivatives on a solid surface

Magnesium oxide/phosphorus oxychloride (MOPO) was found to be an efficient reagent for the preparation of 1,5-benzodiazepine derivatives of o-phenylenediamine and ketones. This method is an easy, rapid, and high yielding reaction for the synthesis of 1,5-benzodiazepine derivatives.© Elsevie

Surface-mediated solid phase reactions: Microwave assisted Arbuzov rearrangement on the solid surface

Microwave assisted Arbuzov rearrangement under solvent-free condition was found to be an efficient method for the preparation of dialkyl alkylphosphonates of alkyl halides. This method is an easy, rapid, and high-yielding reaction for the Arbuzov rearrangement