Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus

Dengue disease is an increasing global health problem that threatens one-third of the world's population. Despite decades of efforts, no licensed vaccine against dengue is available. With the aim to develop an affordable vaccine that could be used in young populations living in tropical areas, we evaluated a new strategy based on the expression of a minimal dengue antigen by a vector derived from pediatric live-attenuated Schwarz measles vaccine (MV). As a proof-of-concept, we inserted into the MV vector a sequence encoding a minimal combined dengue antigen composed of the envelope domain III (EDIII) fused to the ectodomain of the membrane protein (ectoM) from DV serotype-1. Immunization of mice susceptible to MV resulted in a long-term production of DV1 serotype-specific neutralizing antibodies. The presence of ectoM was critical to the immunogenicity of inserted EDIII. The adjuvant capacity of ectoM correlated with its ability to promote the maturation of dendritic cells and the secretion of proinflammatory and antiviral cytokines and chemokines involved in adaptive immunity. The protective efficacy of this vaccine should be studied in non-human primates. A combined measles–dengue vaccine might provide a one-shot approach to immunize children against both diseases where they co-exist

Ælnod. Et Bindeled mellem engelsk og dansk Historieskrivning i 12. Aarh.

International audienc

Ontogeny of LH and FSH receptors in mammalian testis: testosterone secretion

International audienc

Gonadotropin and testosterone patterns and ontogeny of testicular LH/FSH receptors in mammalian fetus

International audienc

RGD-mediated adhesion of porcine granulosa cells modulates their differentiation response to FSH in vitro

International audienc

Ontogeny of gonadal luteinizing hormone and follicle-stimulating hormone receptors in the fetal pig and related changes in gonadotropin and testosterone secretion

International audienc

Ontogeny of gonadal luteinizing hormone and follicle-stimulating hormone receptors in the fetal pig and related changes in gonadotropin and testosterone secretion

International audienc

Ontogenese des récepteurs de la LH et de la FSH et sécrétion de la testostérone au cours du développement foetal

National audienc

Reconstitution of a High-Affinity Functional Lutropin Receptor by Coexpression of Its Extracellular and Membrane Domains

International audienc

LH receptor RNA and protein levels after hormonal treatment of porcine granulosa cells in primary culture

International audienc