Adhesive organ regeneration in <i>Macrostomum lignano</i>

BackgroundFlatworms possess pluripotent stem cells that can give rise to all cell types, which allows them to restore lost body parts after injury or amputation. This makes flatworms excellent model systems for studying regeneration. In this study, we present the adhesive organs of a marine flatworm as a simple model system for organ regeneration. Macrostomum lignano has approximately 130 adhesive organs at the ventral side of its tail plate. One adhesive organ consists of three interacting cells: one adhesive gland cell, one releasing gland cell, and one modified epidermal cell, called an anchor cell. However, no specific markers for these cell types were available to study the regeneration of adhesive organs.ResultsWe tested 15 commercially available lectins for their ability to label adhesive organs and found one lectin (peanut agglutinin) to be specific to adhesive gland cells. We visualized the morphology of regenerating adhesive organs using lectin- and antibody staining as well as transmission electron microscopy. Our findings indicate that the two gland cells differentiate earlier than the connected anchor cells. Using EdU/lectin staining of partially amputated adhesive organs, we showed that their regeneration can proceed in two ways. First, adhesive gland cell bodies are able to survive partial amputation and reconnect with newly formed anchor cells. Second, adhesive gland cell bodies are cleared away, and the entire adhesive organ is build anew.ConclusionOur results provide the first insights into adhesive organ regeneration and describe ten new markers for differentiated cells and tissues in M. lignano. The position of adhesive organ cells within the blastema and their chronological differentiation have been shown for the first time. M. lignano can regenerate adhesive organs de novo but also replace individual anchor cells in an injured organ. Our findings contribute to a better understanding of organogenesis in flatworms and enable further molecular investigations of cell-fate decisions during regeneration

Spin Correlation Coefficients in pp-->pnpi+ from 325 to 400 MeV

The spin correlation coefficient combinations Axx + Ayy, Axx - Ayy and the analyzing powers Ay(theta) were measured for pp-->pnpi+ at beam energies of 325, 350, 375 and 400 MeV. A polarized internal atomic hydrogen target and a stored, polarized proton beam were used. These polarization observables are sensitive to contributions of higher partial waves. A comparison with recent theoretical calculations is provided.Comment: 8 Pages, 1 Table, 5 Figures. Accepted for publication in Phys. Lett.

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MRI guided cryoablation: in vivo assessment of measurin

Spin correlations in p⃗p⃗→pnπ+\vec{p}\vec{p}\to pn\pi^{+} pion production near threshold

A first measurement of longitudinal as well as transverse spin correlation coefficients for the reaction $\vec{p}\vec{p}\to pn\pi^+$ was made using a polarized proton target and a polarized proton beam. We report kinematically complete measurements for this reaction at 325, 350, 375 and 400 MeV beam energy. The spin correlation coefficients $A_{xx}+A_{yy}, A_{xx}-A_{yy}, A_{zz}, A_{xz},$ and the analyzing power $A_{y},$ as well as angular distributions for $\sigma(\theta_{\pi})$ and the polarization observables $A_{ij}(\theta_{\pi})$ were extracted. Partial wave cross sections for dominant transition channels were obtained from a partial wave analysis that included the transitions with final state angular momenta of $l\leq 1$. The measurements of the ${\vec{p}\vec{p}\to pn\pi^{+}}$ polarization observables are compared with the predictions from the J\"ulich meson exchange model. The agreement is very good at 325 MeV, but it deteriorates increasingly for the higher energies. At all energies agreement with the model is better than for the reaction ${\vec{p}\vec{p}\to pp\pi^{0}}$.Comment: Preprint, 21 pp, submitted to Phys. Rev. C. Keywords: Mesons, Polarization, Spin Correlations, Few body system

Preheated Advection Dominated Accretion Flow

All high temperature accretion solutions including ADAF are physically thick, so outgoing radiation interacts with the incoming flow, sharing as much or more resemblance with classical spherical accretion flows as with disk flows. We examine this interaction for the popular ADAF case. We find that without allowance for Compton preheating, a very restricted domain of ADAF solution is permitted and with Compton preheating included a new high temperature PADAF branch appears in the solution space. In the absence of preheating, high temperature flows do not exist when the mass accretion rate mdot == Mdot c^2 / L_E >~ 10^-1.5. Below this mass accretion rate, a roughly conical region around the hole cannot sustain high temperature ions and electrons for all flows having mdot >~ 10^-4, which may lead to a funnel possibly filled with a tenuous hot outgoing wind. If the flow starts at large radii with the usual equilibrium temperature ~10^4 K, the critical mass accretion rate is much lower, mdot \~10^-3.7 above which level no self-consistent ADAF (without preheating) can exist. However, above this critical mass accretion rate, the flow can be self-consistently maintained at high temperature if Compton preheating is considered. These solutions constitute a new branch of solutions as in spherical accretion flows. High temperature PADAF flows can exist above the critical mass accretion rate in addition to the usual cold thin disk solutions. We also find solutions where the flow near the equatorial plane accretes normally while the flow near the pole is overheated by Compton preheating, possibly becoming, a polar wind, solutions which we designate WADAF.Comment: 41 pages with 10 postscript figures (aastex5). Submitted to Ap

Dependence of pp->pp pi0 near Threshold on the Spin of the Colliding Nucleons

A polarized internal atomic hydrogen target and a stored, polarized beam are used to measure the spin-dependent total cross section Delta_sigma_T/sigma_tot, as well as the polar integrals of the spin correlation coefficient combination A_xx-A_yy, and the analyzing power A_y for pp-> pp pi0 at four bombarding energies between 325 and 400 MeV. This experiment is made possible by the use of a cooled beam in a storage ring. The polarization observables are used to study the contribution from individual partial waves.Comment: 6 pages, 1 table, 4 figures, corrected equations 2 and

Novel Silicon Carbide Detector for Active Inspections

The need to address increasingly challenging inspection requirements (such as large volume objects, very fast inspection throughputs, potentially significant shielding, etc.) for such items as nuclear materials and explosives will require the use of active interrogation technologies. While these active technologies can successfully address these challenges by inducing unique, temporal signatures, the inspection environment will also induce overall “background signals” that can be orders of magnitude larger than the induced signatures. Detectors that can successfully operate in these types of customized, inspection environments (pulsed and continuous) and successfully extract induced signature data are clearly needed and will effectively define the limitations of any active inspection system. A novel silicon carbide detector is now being investigated to successfully address both neutron- and photon/bremsstrahlung-type inspection applications. While this paper describes this detector and highlights efforts related to neutron inspection, it will focus on its neutron and gamma-ray/photon detection performance in neutron- and bremssstrahlung-type inspection applications

Observation of strong final-state effects in pi+ production in pp collisions at 400 MeV

Differential cross sections of the reactions $pp \to d\pi^+$ and $pp \to pn\pi^+$ have been measured at $T_p = 400$ MeV by detecting the charged ejectiles in the angular range $4^0 \leq \Theta_{Lab} \leq 21^\circ$. The deduced total cross sections agree well with those published previously for neighbouring energies. The invariant mass spectra are observed to be strongly affected by $\Delta$ production and $NN$ final-state interaction. The data are well described by Monte Carlo simulations including both these effects. The ratio of $pp \to pn\pi^+$ and $pp \to d\pi^+$ cross sections also compares favourably to a recent theoretical prediction which suggests a dominance of $np$-production in the relative $^3S_1$-state.Comment: 17 pages, 5 figure

The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa

Background: Marine biological adhesives are a promising source of inspiration for biomedical and industrial applications. Nevertheless, natural adhesives and especially temporary adhesion systems are mostly unexplored. Sea stars are able to repeatedly attach and detach their hydraulic tube feet. This ability is based on a duo-gland system and, upon detachment, the adhesive material stays behind on the substrate as a 'footprint'. In recent years, characterization of sea star temporary adhesion has been focussed on the forcipulatid species Asterias rubens. Results: We investigated the temporary adhesion system in the distantly related valvatid species Asterina gibbosa. The morphology of tube feet was described using histological sections, transmission-, and scanning electron microscopy. Ultrastructural investigations revealed two adhesive gland cell types that both form electron-dense secretory granules with a more lucid outer rim and one de-adhesive gland cell type with homogenous granules. The footprints comprised a meshwork on top of a thin layer. This topography was consistently observed using various methods like scanning electron microscopy, 3D confocal interference microscopy, atomic force microscopy, and light microscopy with crystal violet staining. Additionally, we tested 24 commercially available lectins and two antibodies for their ability to label the adhesive epidermis and footprints. Out of 15 lectins labelling structures in the area of the duo-gland adhesive system, only one also labelled footprints indicating the presence of glycoconjugates with α-linked mannose in the secreted material. Conclusion: Despite the distant relationship between the two sea star species, the morphology of tube feet and topography of footprints in A. gibbosa shared many features with the previously described findings in A. rubens. These similarities might be due to the adaptation to a benthic life on rocky intertidal areas. Lectin- and immuno-labelling indicated similarities but also some differences in adhesive composition between the two species. Further research on the temporary adhesive of A. gibbosa will allow the identification of conserved motifs in sea star adhesion and might facilitate the development of biomimetic, reversible glues.</p