Bioconjugation of carbohydrates to gelatin sponges promoting 3D cell cultures

Gelatin sponges are widely employed as hemostatic agents, and are gaining increasing interest as 3D scaffolds for tissue engineering. To broaden their possible application in the field of tissue engineering, a straightforward synthetic protocol able to anchor the disaccharides, maltose and lactose, for specific cell interactions was developed. A high conjugation yield was confirmed by 1H-NMR and FT-IR spectroscopy, and the morphology of the resulting decorated sponges was characterized by SEM. After the crosslinking reaction, the sponges preserve their porous structure as ascertained by SEM. Finally, HepG2 cells cultured on the decorated gelatin sponges show high viability and significant differences in the cellular morphology as a function of the conjugated disaccharide. More spherical morphologies are observed when cultured on maltose-conjugated gelatin sponges, while a more flattened aspect is discerned when cultured onto lactose-conjugated gelatin sponges. Considering the increasing interest in small-sized carbohydrates as signaling cues on biomaterial surfaces, systematic studies on how small carbohydrates might influence cell adhesion and differentiation processes could take advantage of the described protocol

Thermal characterization of intumescent fire retardant paints

Intumescent coatings are now the dominant passive fire protection materials used in industrial and commercial buildings. The coatings, which usually are composed of inorganic components contained in a polymer matrix, are inert at low temperatures and at higher temperatures, they expand and degrade to provide a charred layer of low conductivity materials. The charred layer, which acts as thermal barrier, will prevent heat transfer to underlying substrate. The thermal properties of intumescent paints are often unknown and difficult to be estimated since they vary significantly during the expansion process; for this reason the fire resistance validation of a commercial coatings is based on expensive, large-scale methods where each commercial coating-beam configuration has to be tested one by one. Adopting, instead, approaches based on a thermal modelling of the intumescent paint coating could provide an helpful tool to make easier the test procedure and to support the design of fire resistant structures as well. The present investigation is focused on the assessment of a methodology intended to the restoration of the equivalent thermal conductivity of the intumescent layer produced under the action of a cone calorimetric apparatus. The estimation procedure is based on the inverse heat conduction problem approach, where the temperature values measured at some locations inside the layer during the expansion process are used as input known data. The results point out that the equivalent thermal conductivity reached by the intumescent material at the end of the expansion process significantly depends on the temperature while the initial thickness of the paint does not seem to have much effect

Non-Perturbative Renormalisation of Composite Operators

It is shown that the renormalisation constants of two quark operators can be accurately determined (to a precision of a few per-cent using 18 gluon configurations) using Chiral Ward identities. A method for computing renormalisation constants of generic composite operators without the use of lattice perturbation theory is proposed.Comment: 3 pages, uuencoded compressed postscript file, to appear in the Proceedings of the International Symposium on Lattice Field Theory, Dallas, Texas, 12-17 October 1993, Southampton Preprint 93/94-0

Parameter estimation approach to the thermal characterization of intumescent fire retardant paints

Intumescent paints are widely used as passive fire retardant materials in the building sector. They swell on heating to form a highly insulating char, protecting steel members. Intumescent coatings for use in buildings are typically certified according to the standard cellulosic fire resistance test. This test is expensive, often non-representative of realistic fire conditions, and not enough versatile to gather detailed performance information on the response of reactive coatings. A promising approach, that could offer a helpful tool to the engineering community involved in fire safety, is found in the modelling of the behaviour of the intumescent coating. Under this approach, the knowledge of the equivalent thermal conductivity of the intumescent material is a fundamental issue, since it represents the main parameter that allows predicting the thermal protecting capability of the layer. The purpose of this paper is to optimize an estimation procedure intended to the restoration of the equivalent thermal conductivity of intumescent layers. The thermal stress is activated by the action of a cone calorimetric apparatus, while the estimation procedure is based on the inverse heat conduction problem approach under steady state assumption, where the temperature values measured at some locations inside the layer during the expansion process are used as input known data. This procedure was successfully applied to steel samples protected with an intumescent paint; the estimated equivalent thermal conductivity of the layer results to temperature dependent while the initial thickness of the paint does not seem to have a great effect

Current Renormalisation Constants with an O(a)-improved Fermion Action

Using chiral Ward identities, we determine the renormalisation constants of bilinear quark operators for the Sheikholeslami-Wohlert action lattice at beta=6.2. The results are obtained with a high degree of accuracy. For the vector current renormalisation constant we obtain Z_V=0.817(2)(8), where the first error is statistical and the second is due to mass dependence of Z_V. This is close to the perturbative value of 0.83. For the axial current renormalisation constant we obtain Z_A = 1.045(+10 -14), significantly higher than the value obtained in perturbation theory. This is shown to reduce the difference between lattice estimates and the experimental values for the pseudoscalar meson decay constants, but a significant discrepancy remains. The ratio of pseudoscalar to scalar renormalisation constants, Z_P/Z_S, is less well determined, but seems to be slightly lower than the perturbative value.Comment: 8 pages uuencoded compressed postscript file. Article to be submitted to Phys.Rev.