Spin and quadrupole contributions to the motion of astrophysical binaries

Compact objects in general relativity approximately move along geodesics of spacetime. It is shown that the corrections to geodesic motion due to spin (dipole), quadrupole, and higher multipoles can be modeled by an extension of the point mass action. The quadrupole contributions are discussed in detail for astrophysical objects like neutron stars or black holes. Implications for binaries are analyzed for a small mass ratio situation. There quadrupole effects can encode information about the internal structure of the compact object, e.g., in principle they allow a distinction between black holes and neutron stars, and also different equations of state for the latter. Furthermore, a connection between the relativistic oscillation modes of the object and a dynamical quadrupole evolution is established.Comment: 43 pages. Proceedings of the 524. WE-Heraeus-Seminar "Equations of Motion in Relativistic Gravity". v2: fixed reference. v3: corrected typos in eqs. (1), (57), (85

Shiga Toxin Binding to Glycolipids and Glycans

Background: Immunologically distinct forms of Shiga toxin (Stx1 and Stx2) display different potencies and disease outcomes, likely due to differences in host cell binding. The glycolipid globotriaosylceramide (Gb3) has been reported to be the receptor for both toxins. While there is considerable data to suggest that Gb3 can bind Stx1, binding of Stx2 to Gb3 is variable. Methodology: We used isothermal titration calorimetry (ITC) and enzyme-linked immunosorbent assay (ELISA) to examine binding of Stx1 and Stx2 to various glycans, glycosphingolipids, and glycosphingolipid mixtures in the presence or absence of membrane components, phosphatidylcholine, and cholesterol. We have also assessed the ability of glycolipids mixtures to neutralize Stx-mediated inhibition of protein synthesis in Vero kidney cells. Results: By ITC, Stx1 bound both Pk (the trisaccharide on Gb3) and P (the tetrasaccharide on globotetraosylceramide, Gb4), while Stx2 did not bind to either glycan. Binding to neutral glycolipids individually and in combination was assessed by ELISA. Stx1 bound to glycolipids Gb3 and Gb4, and Gb3 mixed with other neural glycolipids, while Stx2 only bound to Gb3 mixtures. In the presence of phosphatidylcholine and cholesterol, both Stx1 and Stx2 bound well to Gb3 or Gb4 alone or mixed with other neutral glycolipids. Pre-incubation with Gb3 in the presence of phosphatidylcholine and cholesterol neutralized Stx1, but not Stx2 toxicity to Vero cells

The nature and development of aviation insurance ...

Mode of access: Internet