Expression, production and purification of proteinases from microbes

Screening and expression of protease producing 66 strains of different microbes were obtained from the various places in Chennai, Vellore, Tamilnadu, India. The isolates were positive on tyrosin caesin nitrate agar (1%) and thus are selected as protease producing strain. 11 of them belong to miscellaneous microbes. The microbial growth is revealed by the mycelial dry weight determination. Maximum growth is observed in the case of Bacillus (0.78 mg). Equally, the maximum growth is observed in Aspergillus sp. (0.064 mg), Fusarium sp. (0.62 mg) and Penicillium sp. (0.62 mg). Finally the enzyme protease was purified by column chromatography. The protein was characterized using SDS-PAGE. Maximum protein content is observed in the case of Alternaria sp. (0.902mg) and Penicillium sp. (0.624 mg). Maximum proteinase content is observed in Aspergillus sp. (0.866mg). This results showed that microbes under study is a good producer of extra cellular protease, which can be beneficial for industries.Keywords: Expression; production; purification; proteinase

Expression, production and purification of proteinases from microbes

Screening and expression of protease producing 66 strains of different microbes were obtained from the various places in Chennai, Vellore, Tamilnadu, India. The isolates were positive on tyrosin caesin nitrate agar (1%) and thus are selected as protease producing strain. 11 of them belong to miscellaneous microbes. The microbial growth is revealed by the mycelial dry weight determination. Maximum growth is observed in the case of Bacillus (0.78 mg). Equally, the maximum growth is observed in Aspergillus sp. (0.064 mg), Fusarium sp. (0.62 mg) and Penicillium sp. (0.62 mg). Finally the enzyme protease was purified by column chromatography. The protein was characterized using SDS-PAGE. Maximum protein content is observed in the case of Alternaria sp. (0.902mg) and Penicillium sp. (0.624 mg). Maximum proteinase content is observed in Aspergillus sp. (0.866mg). This results showed that microbes under study is a good producer of extra cellular protease, which can be beneficial for industries.Keywords: Expression; production; purification; proteinase

AEROBIOLOGY OF MULBERRY FIELDS IN VELLORE DISTRICT

ABSTRACT Mulberry which forms the exclusive source of food for commercial silk producing silk worm (Bombyx mori L.) is affected by a number of diseases and pests. The aerobiology of crop field was very limited and much of the fields were to be opened for investigation. It affects the nutrition, growth and cocoon production of silkworm. Hence a detailed investigation was carried out on the aerobiology of mulberry fields in Vellore district. The Vellore district comprises of eight taluks, 14 ranges and 64 subdivisions. Much of the Vellore district land area is cultivated by Mulberry as a cottage industry. It is found that from October to January rainfall was maximum, Vaniyambadi, Tirupattur and Gudiyattam receives maximum rain. The humidity is relatively maximum during the period of October to September 1999. The temperature was maximum from March to June. This helps for the spread and growth of various microflora and airspores in Vellore district environs. From these data, the causative organisms of various diseases of Mulberry were identified. This data will be helpful for the treatment, management of the much useful plant Mulberry in Vellore district

Physicochemical Characteristics, Identification of Fungi and Optimization of Different Parameters for Degradation of Dye from Tannery Effluent

This study was carried out to find out the qualities of tannery effluents with the assessment of physicochemical parameters of effluent, isolation, and identification of fungi and their optimization of different parameters on dye decolorization. In the present study, various physicochemical parameters such as Color, Odor, pH, EC, TSS, TDS, BOD, COD, Chromium, Copper, Chloride, and Sodium of untreated tannery effluent was studied. The results of the parameters showed that the effluent was blackish with a disagreeable odor, alkaline in pH with a high organic and inorganic loads such as EC, TDS, BOD, COD, TSS, Chromium, Copper, Chloride and Sodium. The physicochemical parameters were determined as per the standards prescribed by CPCB. Four fungal species were isolated and identified by LPCB staining namely Aspergillus niger , Aspergillus flavus , Penicillium citrinum , and Curvularia lunata . To test the activity of these fungi on different dyes, experiments were carried out for the optimization of different parameters. The maximum decolorization of dye was achieved by Aspergillus niger. From this study, it was found that the maximum biotransformation of dye effluent can help to solve the pollution problem

Character and spatial distribution of OH/H<SUB>2</SUB>O on the surface of the moon seen by M<SUP>3</SUP> on Chandrayaan-1

The search for water on the surface of the anhydrous Moon had remained an unfulfilled quest for 40 years. However, the Moon Mineralogy Mapper (M3) on Chandrayaan-1 has recently detected absorption features near 2.8 to 3.0 micrometers on the surface of the Moon. For silicate bodies, such features are typically attributed to hydroxyl- and/or water-bearing materials. On the Moon, the feature is seen as a widely distributed absorption that appears strongest at cooler high latitudes and at several fresh feldspathic craters. The general lack of correlation of this feature in sunlit M3 data with neutron spectrometer hydrogen abundance data suggests that the formation and retention of hydroxyl and water are ongoing surficial processes. Hydroxyl/water production processes may feed polar cold traps and make the lunar regolith a candidate source of volatiles for human exploration

Alternative low-cost adsorbent for water and wastewater decontamination derived from eggshellwaste: an overview

As the current global trend towards more stringent environmental standards, technical applicability and cost-effectiveness became key factors in the selection of adsorbents for water and wastewater treatment. Recently, various low-cost adsorbents derived from agricultural waste, industrial by-products or natural materials, have been intensively investigated. In this respect, the eggshells from egg-breaking operations constitute significant waste disposal problems for the food industry, so the development of value-added by-products from this waste is to be welcomed. The egg processing industry is very competitive, with low profit margins due to global competition and cheap imports. Additionally, the costs associated with the egg shell disposal (mainly on landfill sites) are significant, and expected to continue increasing as landfill taxes increase. The aim of the present review is to provide an overview on the development of low-cost adsorbents derived from eggshell by-products

Towards a Rosetta Stone for translating data between information systems

This article was accepted for publication in the journal, Business Information Review [Sage Publications / © The Authors]. The definitive version is available at: http://dx.doi.org/10.1177/0266382115616235Information systems are an important organizational asset and offer numerous benefits. However, organizations face continued challenges when upgrading ageing information systems, and the data contained within, to newer platforms. This article explores, through conversations with information systems professionals in four organizations, the potential development of a ‘Rosetta Stone’, which can translate data between systems and be used to help overcome various challenges associated with their modernization. Despitemixed feedback regarding theRosetta Stone concept from interviewees, solutions highlighted in literature combinedwith participant feedback presented theories for its development, primarily as a tool to enable meaningful interpretation of data, rather than direct translation. The conclusion reflects on data collected to recommend a framework for how the tool might be developed and has the potential to be of significant interest to practitioners, open-source communities and organizations