5 research outputs found
The profile of ErbB/Her family genes copy number assessed by real-time PCR in parathyroid adenoma and hyperplasia associated with sporadic primary hyperparathyroidism
Hyperparathyroidism (pHPT) is a relatively frequent endocrinopathy, however, the molecular mechanisms of its etiology remain poorly understood. This disorder is mainly associated with benign tumours (adenoma) and hyperplasia of the parathyroid, hence, the focus is directed also to genes that are likely to be involved in carcinogenesis. Among such genes are ErbB/Her family genes already used in diagnosis of other tumours (e.g., breast carcinoma) and reported also to play a role in development of endocrine lesions. So far, ErbB-1/Her-1/EGFR expression has been detected in pHPT-associated adenomas and hyperplasia as opposed to no expression in normal parathyroid tissue. Moreover, losses or gains of the fragments of chromosomes where ErbB/Her genes are located have been reported. In this study, the gene dosage of ErbB/Her family genes were determined for the first time in parathyroid adenomas, hyperplasia and morphologically unchanged tissue in order to establish their putative role in the development of the disease. Genomic DNA was isolated from 33 patients with sporadic hyperparathyroidism and the gene copy numbers were assessed using real-time PCR. The ErbB/Her genes' profile was unaltered in most of the examined samples. Two low-level amplifications of ErbB-1/Her-1/EGFR gene, two deletions of ErbB-2/Her-2, and six deletions of ErbB-4/Her-4 were found. The ErbB-3/Her-3 gene remained unaffected. No correlation with clinical parameters was found for any gene. Both the low number of alterations and a lack of their associations with clinical parameters exclude the prognostic value of the ErbB/Her genes family in parathyroid tumourigenesis. Nevertheless, the ErbB-4/Her-4 deletions seem to be interesting for further investigations, especially in the context of PTH secretion
Assessment of real-world usage of lanreotide AUTOGEL 120 in Polish acromegalic patients - results from
Aim of the study: To assess resource utilization
and costs of treatment with lanreotide
AUTOGEL 120 mg (ATG120)
administered as part of routine acromegaly care in Poland.
Material and methods: A multicentre,
non-interventional, observational study
on resource utilization in Polish acromegalic
patients treated with ATG120 at
4 weeks or extended (> 4 weeks) dosing
interval. The study recruited adult acromegalic patients treated medically for
≥ 1 year including at least 3 injections of
ATG120. Data on dosing interval, aspects
of administration, and resource utilization
were collected prospectively during
12 months. Costs were calculated in PLN
from the public health-care payer perspective
for the year 2013.
Results: 139 patients were included in
the analysis. Changes in dosing regimen
were reported in 14 (9.4%) patients. Combined
treatment was used in 11 (8%) patients. Seventy patients (50%) received
ATG120 at an extended dosing interval;
the mean number of days between
injections was 35.56 (SD 8.4). ATG120
was predominantly administered in an
out-patient setting (77%), by health-care
professionals (94%). Mean time needed
for preparation and administration was
4.33 and 1.58 min, respectively, mean
product wastage - 0.13 mg. Patients were
predominantly treated in an out-patient
setting with 7.06 physician visits/patient/
year. The most common control examinations
were magnetic resonance imaging
of brain and brain stem (1.36/
patient/year), ultrasound of the neck
(1.35/patient/year), GH (1.69/pa tient/
year), glycaemia (1.12/patient/year),
IGF-1 (0.84/patient/year), pituitary-thyroid
axis hormone levels assessment
(TSH-0.58/patient/year, T4-0.78/patient/
year). There were 0.43 hospitalizations/
patient/year. For direct medical
costs estimated at PLN 50 692/pa tient/
year the main item was the costs of
ATG120 (PLN 4103.87/patient/month;
97%). The mean medical cost, excluding
pharmacotherapy, was PLN 1445/patient/year (out-patient care - 49%, hospitalization
- 23%, diagnostics/laboratory
tests - 28%).
Conclusions: These results represent
the current use of ATG120 in the population
of Polish acromegalic patients in
a realistic clinical setting. Findings that
50% of patients could be treated with
dose intervals of longer than 28 days
support the potential of ATG120 to
reduce the treatment burden