Protective effects of dantrolene and methylprednisolone against spinal cord injury-induced early oxidative damage in rabbit bladder: A comparative experimental study

Conclusions. Dantrolene and MP may have potential benefits against oxidative damage in the bladder after SCIs because of their anti-inflammatory and antioxidant effects. In particular, the combined use of DNT and MP at different doses can be considered a treatment strategy

Involvement of serotonin receptor subtypes in the antidepressant-like effect of beta receptor agonist Amibegron (SR 58611A): An experimental study

New therapeutic strategies against depression, with less side effects and thus greater efficacy in larger proportion of depressed patients, are needed. Amibegron (SR58611A) is the first selective beta 3 adrenergic agent that has been shown to possess a profile of antidepressant activity in rodents. to investigate the involvement of serotonin receptors in the effects of amibegron, we used the serotonin 5HT1A receptor antagonist WAY-100635 (WAY) or serotonin 5HT2A-2C receptor antagonist ketanserin or serotonin 5HT3 receptor antagonist ondansetron in mice forced swimming test (FST). The locomotor activity was evaluated by measuring the total distance moved in the apparatus and the speed of the animals in the open field test. Imipramine (30 mg/kg) significantly reduced immobility time compared to vehicle-treated group while amibegron (5 and 10 mg/kg) dose dependently reduced immobility time in the FST. WAY(0.1 mg/kg), ondansetron (1 mg/kg), ketanserin(5 mg/kg) had no effect on immobility time in naive mice while all of the drugs partially and significantly reversed amibegron (10 mg/kg) induced decreasement in the immobility time in FST. None of the drugs alter locomotor activity in the open field test. The antidepressant-like effect of amibegron in the FST seems to be mediated by an interaction with serotonin 5-HT1A, serotonin 5-HT2A-2C and serotonin 5-HT3 receptors. (C) 2013 Elsevier Inc. All rights reserved

Evidence that the anxiolytic-like effects of the beta3 receptor agonist Amibegron involve serotoninergic receptor activity

Anxiety disorders are the most common behavioral disorders, and they exhibit high comorbidity rates. The aim of the present study was to confirm the effects of Amibegron, the first selective beta 3 adrenergic agent, on anxiety and to demonstrate that different serotoninergic receptor subtypes are involved in this effect. We administered the serotonin 5-HT1A receptor antagonist WAY-100635, the serotonin 5-HT2A receptor antagonist Ketanserin and the serotonin 5-HT3 receptor antagonist Ondansetron in mice and evaluated their performance in the elevated plus-maze test. Mice administered with Amibegron (5 and 10 mg/kg) showed a dose-dependent prolonged time spent in the open arms and an increase in the number of entries into the open arms during the elevated plus-maze (EPM) test. However, in the control mice, administration of WAY, Ketanserin and Ondansetron demonstrated no effect on the time spent in the open arms and the number of entries into the open arms. In addition, these treatments all significantly reversed the effect of the Amibegron-induced (10 mg/kg) increase in the time spent in the open arms. However, only WAY and Ketanserin treatments reversed the Amibegron-induced increase in the number of entries into the open arms. In conclusion, Amibegron exerted a significant anxiolytic effect, which was as effective as Diazepam, in mice during the EPM test. This effect of Amibegron may be mediated by interactions with the serotonin 5-HT1A, 5-HT2A and 5-HT3 receptors. (C) 2013 Elsevier Inc. All rights reserved

Royal jelly can diminish secondary neuronal damage after experimental spinal cord injury in rabbits

The aim of this experimental study was to investigate the neuroprotective effect of Royal jelly (RJ) on traumatic spinal cord injury (SCI). Twenty-one New Zealand male rabbits, weighing between 2.5 and 3.0 kg were divided into three groups: Sham (no drug or operation, n = 7), Control (laminectomy + single dose of 1 ml/kg saline orally, after trauma; n = 7) and RJ (laminectomy + 100 mg/kg RJ, orally, after trauma, n = 7). Laminectomy was performed at T10 and balloon catheter was applied extradurally for traumatic SCI. Four and 24 h after surgery, rabbits were evaluated according to the Tarlov scoring system. Blood, cerebrospinal fluid and tissue sample from spinal cord were taken for measurements of antioxidant status or detection of apoptosis. Four hours after SCI, all animals in control or RJ treated groups became paraparesic. Significant improvement was observed in RJ treated group, 24 h after SCI, with respect to control. Traumatic SCI led to increase in the lipid peroxidation and decrease enzymic or non-enzymic endogenous antioxidative defense systems, and increase in apoptotic cell numbers. RJ treatment mostly prevented lipid peroxidation and also augmented endogenous enzymic or non-enzymic antioxidative defense systems. Again, RJ treatment significantly decreased the apoptotic cell number induced by SCI. (C) 2012 Elsevier Ltd. All rights reserved

The effects of ethanol on glucose 6-phosphate dehydrogenase enzyme activity from human erythrocytes in vitro and rat erythrocytes in vivo

Aims: The effects of ethanol on erythrocyte glucose 6-phosphate dehydrogenase (G6PD) activity were investigated under in vitro and in vivo conditions. Methods: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method. Results: The in vitro study showed that the I-50 value was 17 mM for ethanol. In the case of the in vivo study, a 2 ml/kg dose of ethanol significantly inhibited the G6PD activity. The inhibition rate after ethanol administration was 59%, 40% and 6% at 1, 3 and 6 h after, respectively. Conclusions: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro

Klorpromazin ve Haloperidol’ün İnsan Eritrosit Glukoz 6-Fosfat Dehidrogenaz Enzimi Üzerine İnvitro Etkileri

Klorpromazin ve haloperidolün in vitro koflullarda insaneritrosit glukoz 6-fosfat dehidrogenaz enzimi üzerine etkileribelirlemek amac›yla bu çal›flma yap›ld›. Yöntem: Glukoz 6-fosfatdehidrogenaz enzimi insan eritrositlerinden saflaflt›r›ld›. Enzimaktivitesinin tayini Beutler metoduna göre spektrofotometrikolarak 340nm ‘de yap›ld›. Bulgular: Çal›flman›n sonuçlar›na göreI50 de¤erleri klorpromazin için 0,42 mM, haloperidol için 0,37mM oldu. Sonuç: Elde edilen veriler göstermektedir ki, klorpromazinve haloperidol glukoz 6-fosfat dehidrogenaz enzimininaktivitesini in vitro olarak güçlü bir flekilde inhibe etmifltir.Anahtar sözcükler: klorpromazin, haloperidol,Effects of chlorpromazine and haloperidol on erythrocyteglucose 6-phosphate dehydrogenase enzyme activitywere investigated in invitro conditions. Methods: Glucose 6-phosphate dehydrogenase was purified from human erythrocyte.Enzyme activity was determined according to the Beutlermethod by using a spectrophotometer at 340 nm. Results: Theresults of study showed that I50 values were 0,42 mM for chlorpromazineand 0,37 mM for haloperidol in invitro study.Conclusion: The results of this study suggested that chlorpromazineand haloperidol have significant inhibition effect on theactivity of glucose 6-phosphate dehydrogenase enzyme in invitro.Key words: chlorpromazine, haloperidol, glucose 6-phosphatedehydrogenase, erythrocyte, in vitro</div