Selection for resistance against root pathogens in a pea composite cross

The possibility of improving resistance in pea against the root pathogen Aphanomyces euteiches using composite cross as a breeding and selection method was examined. In order to maintain acceptable agricultural features and high yield 6 out of the 8 parental varieties in the present composite-cross were commercially grown varieties. Populations of the composite cross were grown up to five generations with selection pressure in soil heavily infested with pea root pathogens or without selection pressure on soil free of pea root pathogens. Yield of populations of the F9 and F10 generations of the composite cross grown with selection pressure was on average 35% higher than that of the population obtained without selection pressure as well as the average yield of the 8 parentals of the composite cross, which were of similar magnitude. In healthy soil the yield was overall higher than in the pathogen-infested soil, but yield did not differ between the populations from the composite cross with and without selection pressure, which were also similar to the average yield of the 8 different parentals. Recombinant inbred lines (RILs) randomly selected from the F10 population with selection pressure developed 23% less root rot than the corresponding F10 population without selection pressure, when grown in field soil heavily infested with pea root pathogens. Surprisingly, greenhouse pot experiments with pure cultures of the pea root pathogen A. euteiches resulted in higher root disease, in RILs from populations with selection pressure than from corresponding RILs without selection pressure. Problems related to greenhouse screening for resistance is discussed as well as the possibilities of using composite cross as a method to improve resistance against root diseases in grain legumes

Specificity of soil-borne pathogens on grain legumes

Specificity of soil-borne legume pathogens on pea, lupin and faba bean is currently investigated in fields where grain legumes are intensively cultivated. The study has so far lead to the following conclusions -Legume host-pathogen interactions demonstrate specificity of pathogen populations particularly in pea and lupin. -A. euteiches rot root was specified to pea in Denmark as root rot symptoms and oospores of the pathogen never were observed in roots of faba bean and lupin -F. oxysporum followed by F. solani were most frequently isolated from plant roots in plots highly infested by lupin pathogens -F. avenaceum was most frequently isolated from plant roots in plots highly infested by pea pathogens -Pathogenicity tests showed F. solani followed by F. avenaceum to be the most pathogenic Fusarium species on pea while F. avenaceum was the most destructive pathogen on faba bean. In contrast F. avenaceum was non-pathogenic on lupin

Svampsjukdomar på ärter

Svampsjukdomar kan angripa ärter på rötter, stjälkar, blad, baljor och frön. Rotsjukdomar uppträder antingen vid användning av infekterat utsäde eller på platser där man tidigare har odlat ärter flera gånger. Bladsjukdomarna däremot kan spridas med vinden mellan fålt. Bladsjukdomarna är förhållandevis lätta att identifiera, medan rot-och stjälkbassjukdomarna är svårare att särskilja eftersom de ofta förekommer samtidigt på rötterna

Population genomics of an outbreak of the potato late blight pathogen, <i>Phytophthora infestans</i>, reveals both clonality and high genotypic diversity

An outbreak of the potato late blight pathogen Phytophthora infestans in Denmark was characterized in order to resolve the population structure and determine to what extent sexual reproduction was occurring. A standard set of microsatellite simple sequence repeats (SSRs) and single nucleotide polymorphism (SNP) markers generated using restriction site‐associated DNA sequencing (RAD‐seq) were employed in parallel. A total of 83 individuals, isolated from seven different potato fields in 2014, were analysed together with five Danish whole‐genome sequenced isolates, as well as two Mexican individuals used as an outgroup. From a filtered dataset of 55 288 SNPs, population genomics analyses revealed no sign of recombination, implying clonality. In spite of this, multilocus genotypes were unique to individual potato fields, with little evidence of gene flow between fields. Ploidy analysis performed on the SNPs dataset indicated that the majority of isolates were diploid. These contradictory results with clonality and high genotypic diversity may suggest that rare sexual events likely still contribute to the population. Comparison of the results generated by SSRs vs SNPs data indicated that large marker sets, generated by RAD‐seq, may be advised going forward, as it provides a higher level of genetic discrimination than SSRs

Cell-based therapy for the treatment of female stress urinary incontinence:an early cost–effectiveness analysis

Aim: To perform an early cost–effectiveness analysis of in vitro expanded myoblasts (IVM) and minced myofibers versus midurethral slings (MUS) for surgical treatment of female stress urinary incontinence. Methods: Cost–effectiveness and sensitivity analyses were performed using a decision tree comprising previously published data and expert opinions. Results &amp; conclusion: In the base case scenario, MUS was the cost-effective strategy with a negative incremental cost–effectiveness ratio compared with IVM and a positive incremental cost–effectiveness ratio compared with minced myofibers. However, the sensitivity analysis indicates that IVM may become an alternative providing greater effect at a higher cost. With the possibility of becoming more effective, IVM treatment would be advantageous over MUS given its reduced invasiveness and lower risks of complications. </jats:p