幼若期ラットの腎臓に及ぼす飼料中カルシウム量とリン量の影響

Kidney calcium contents and the calcium balance were studied in young female F344 rats, using a combination of calcium and phosphorus at each levels. Enlargement and pathological findings of the kidney were also observed. The results were as follows: 1) An increase in phosphorus intake alone caused a decrease in urinary calcium excretion. 2)A high phosphorus intake caused a significant increase in kidney weight. 3)A low calcium or a high phosphorus intake caused a significant increase in kidney weight and calcium content. 4) Pathological examination revealed that calcification and degeneration had occurred in the distal parts of renal tubule according to low calcium or high phosphorus intake. These results indicate that dietary mineral levels may have influence on calcium metabolism of the kidney

雌ラットのカルシウム,リンおよびマグネシウム出納に及ぼす飼料中タンパク質の影響

The experiment was carrid out to observe the effect of dietary protein quantity and its quality on calcium (Ca), phosphorus (P) and magnesium (Mg) balance in female rats. Diets for each 5 experimental groups were 20% and 40% protein levels respectively. Milk casein, soy protein isolate and wheat gluten were used as a protein source. Female fisher-strain rats of 4 weeks of age were divided into groups of 20% casein diet (C-20), 40% casein diet (C-40), 40% soy protein isolated diet (S-40),20% wheat gluten diet (G-20) and 40% wheat gluten diet (G-40) and effects of the diets on Ca, P and Mg balance were observed for 13 weeks. To each diets, first limiting amino acid was added. During the experimental periods, 1st, 5th and 13th weeks, Ca, P and Mg balance was determined. The results are as follow : 1. Body weight gain and food intake were not significantly different between the diets. 2. Absorption rate of Ca, P and Mg decreased with age. 3. Forty percent protein diets always indicated higher urinary Ca excretion compared to 20% protein diets. In addition, urinary Ca levels were higher in order G-40, C-40 and S-40. 4. Retention rate of Ca, P and Mg also decreased with age. The highest Ca retention was observed on C-40 diet and the lowest was G-40 diets

幼若期雌ラットのミネラル出納に及ぼす飼育期間の検討

The effect of feeding period on calcium (Ca) utilization in rats fed on the same experimental design was investigated. The food efficiency varied with the difference of trial period. The deviation of balance test that starting time is different has shown significant difference of Ca absorption, urinary Ca excretion and Ca retention. The weight of kidney and its Ca content varied according to the difference of trial period. The Ca utilization was affected by the difference of feeding period in the same protocol

幼若期ラットのミネラル出納に及ぼす飼料中脂肪量の影響

The purpose of the present study was to investigate the effect of dietary fat levels on calcium, phoshorus and magnesium balance in rats. Young female Fischer 344 rats were fed the AIN-76 diets modified to contain 20% (normal) milk casein. Each of casein diets were given to three level of soybean oil of 5% (normal), 10% (high) and 20% (excess) diets. The calcium, phosphorus and magnesium intake were decreased significantly by the increase of the dietary fat level from 5% and 10% to 20%. The calcium, phosphorus and magnesium absorption were increased significantly by the increase of the dietary fat level from 10% to 20%. The increase of the dietary fat level from 5% and 10% to 20% incresed significantly urinary calcium excretion. However, the calcium and phosphorus retention were increased by increasing the dietary fat level from 10% to 20%. The contents of calcium, phosphorus and magnesium in kidney were significantly increased as the dietary fat levels were increased. These results suggested that calcium balance in rats was especially affected by dietary fat levels

ラットにおけるたんぱく質摂取量が鉄利用に及ぼす影響について

The aim of this experiment is to determine iron (Fe) utilization resulted from protein intake in the Fischer 344 rat. Experimental diets compose of three levels: the low level (10%), normal level (20%) and high level (40%). Fecal and urinary samples are collected using metabolic cages on the final week during the 6 weeks. The measurement of Fe is performed by the method of atomic absorption spectrometry. All results are subject to an analysis of variance; critical level for all statistical analyses is set at p<0.05. In all studies, a rise of the ratio of Fe absorption significantly has been increased by protein intake. The content of iron, total binding capacity, unsaturation binding capacity and saturation factor in serum was not affected by protein intake. Similarly, the content of iron in kidney has not changed significantly