Additional file 1: Figure S1. of Expression of Par3 polarity protein correlates with poor prognosis in ovarian cancer

Par3 is sufficiently knocked down at 24, 48, and 72 h. JHOC cells were transfected with siPar3 or control siRNA (siControl). Total cell extracts were then taken after 24, 48, and 72 h. Par3 and ι-Tubulin protein were detected by western blotting. (PPTX 139 kb

Measurement of the signaling entropy in GSE27678.

<p>(A) Boxplot of the entropy rate from whole samples. The entropy rate increased according to the disease progression (Kruskal-Wallis test, <i>p</i> < 0. 001). Among them, the entropy rate from the cell line was highest, as described in the literature [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0176353#pone.0176353.ref009" target="_blank">9</a>]. (B) Entropy rate from each platform. ○ represents the data obtained from the Affymetrix Human Genome U133A 2.0 Array, and * represents the data obtained from the Affymetrix Human Genome U133 Plus 2.0 Array. The distribution patterns of the entropy rate seemed slightly different among the platforms.</p

Measurement of the signaling entropy in GSE63514.

<p>The entropy rate was significantly higher according to the disease progression (Kruskal-Wallis test, <i>p</i> < 0.0001). Cancer; invasive cancer.</p

Comparison of the number of endometriotic lesions between wild type and 12/15-LOX-KO mice with or without EPA administration.

<p>Endometriotic lesions were generated in wild type (WT: black) and 12/15-LOX-KO (stripe) mice with or without oral administration of 5% EPA ethyl ester. The number of endometriotic lesions was compared between the wild type and 12/15-LOX-KO mice with or without EPA oral administration (n = 4 in each group). Mean values with standard deviations are presented. Asterisks indicate those comparisons with statistical significance (p<0.05).</p

Lipid mediator analyses of peritoneal fluids from wild type or 12/15-LOX-KO mice with or without EPA administration.

<p>Peritoneal exudates of mice developing endometriotic lesions were collected by washing with saline. The peritoneal fluids obtained from mice as shown in Fig.(n = 3 in each group). The main products of AA-, EPA- and DHA-derived mediators were indicated. Y axis denotes the amount of each lipid mediator (pg/g sample). Mean values with standard deviations are presented. Asterisks indicate those comparisons (wild type vs. 12/15-LOX-KO mice) with significance (p<0.05).</p

Lipid mediator analyses of peritoneal fluids: wild type vs. fat-1 mice.

<p>Peritoneal exudates of mice developing endometriotic lesions were collected by washing with saline. The peritoneal fluids obtained from the fat-1 (white) or wild type (WT: black) mice were analyzed as shown in the Fig. 3 (n = 3 in each group). The main products of AA-, EPA- and DHA-derived mediators are indicated. Y axis donates the amount of each lipid mediator (pg/g sample). Mean values with standard deviations are presented. Asterisks indicate those comparisons (fat-1 vs. wild type mice) with statistical significance (p<0.05).</p

Comparison of the cytokine/chemokine profiles of peritoneal endometriotic lesions between fat-1 and wild type (WT) mice (n = 3 in each group).

<p>Comparison of the cytokine/chemokine profiles of peritoneal endometriotic lesions between fat-1 and wild type (WT) mice (n = 3 in each group).</p

Lipid mediator analyses of endometriotic lesions: wild type vs. fat-1 mice.

<p>Endometriotic lesions obtained from fat-1 (white) or wild type (WT: black) mice were assessed by lipidomic analyses (n = 3 in each group). The main products of AA-, EPA- and DHA-derived mediators are indicated. Y axis denotes the amount of each lipid mediator (pg/g sample). Mean values with standard deviations are presented. Asterisks indicate those comparisons (fat-1 vs. wild type mice) with statistical significance (p<0.05).</p

IL-6 production of peritoneal macrophages derived from fat-1 and wild type mice.

<p>Peritoneal fluids were collected from the fat-1 (white) and wild type (WT: black) mice as shown in Fig. 4 (n = 5 in each group). Among them, peritoneal macrophages were isolated by CD11b-beads selection. IL-6 mRNA levels in isolated macrophages were measured by RT-quantitative PCR. IL-6 mRNA levels were normalized to β-actin. Mean values with standard deviations are presented. Asterisks indicate those comparisons (fat-1 vs. wild type mice) with statistical significance (p<0.05).</p

Measurement of the signaling entropy in GSE75132.

<p>The median of the entropy rate was higher in the HPV16-persistent (HPV16+) groups than the HPV-negative (HPV-) group. The group that was destined to progress to CIN3+, or the HPV16-persistent with progression group, tended to have a higher entropy rate than the HPV16-persistent without progression group.</p