Effect of Purple Sweet Potato Extract on Glutathione Peroxidase in House Mice Liver After Maximum Physical Exercise

BACKGROUND: Physical exercise can improve antioxidant defense system of an organism, but this process may take a long time. Heavy exercise may disrupt oxidant-antioxidant balance. Low glutathione peroxidase is a biomarker for free radicals. Plant purple sweet potato (Ipomoea batatas L.) contains high anthocyanin that may act as an antioxidant. This study aimed to determine the effect of purple sweet potato extract on the activity of glutathione peroxidase in the liver of mice (Mus Musculus) treated with maximum physical exercise (MPE). SUBJECT AND METHODS: This was a randomized controlled trial using a sample of 24 white DD Webster strain male mice. This sample of mice was randomized into 6 groups: P1-control, P2-MPE, P3-0.5 ml purple sweet potato, P4-MPE + 0.5 ml purple sweet potato, P5-MPE + 1 ml purple sweet potato, and P6-MPE + 1.5 ml purple sweet potato. The purple sweet potatoes were given for 14 days. Difference in means of glutathione peroxidase among the study groups was tested by Kruskal-Wallis. RESULTS: After 14 days of intervention, the level of glutathione peroxidase (mean ± SD in mU/ml) among the study groups were as follows: P1 (6.58±7.04), P2 (1.84±0.92), P3 (4.50±4.31), P4 (7.05±8.19), P5 (19.39±7.06), P6 (3.03±1.98). The highest level of glutathione peroxidase was attained in group P5 with moderate dose (1 ml) of purple sweet potato extract. CONCLUSION: Moderate dose (1 ml) of purple sweet potato extract can effectively increase glutathione peroxidase enzyme in mice. Keywords: maximum physical exercise, free radical, purple sweet potato, anthocyanin, glutathione peroxidas

Effect of Purple Sweet Potato (Ipomoea Batatas L.) Extract on Glutathione Peroxidase (GPx) Activities in Hepatic House Mice (Mus musculus) After Maximum Physical Exercise

Background: Physical exercise can improve antioxidant defense system of organis. But long and heavy exercise can disrupt the balance of oxidant-antioxidant. Low glutathione peroxidase is associated with free radicals. Plant purple sweet potatoes (Ipomoea batatas L.) contain anthocyanins high enough that act as antioxidants. This stu­dy aimed to determine the effect of extracts of tubers of purple sweet potato (Ipomoea Batatas L.) on the activity of glutathione peroxidase (GPx) liver of mice (Mus Musculus) treated with maximal physical exercise.Subjects and Method: This was a true experimental study. This conducted with the design of the control group post-test only in vivo on 24 white male mice (Mus musculus), DD Webster strain. Da­ta analysis was using SPSS software 19. Statistical testing decision taken 5% significance level (p=0.05). Results: The results showed that the average enzyme activity of GPx in the P5 is the highest enzyme activity GPx (mean=19:39 ± SD=7:06, p=0.024), which means there are significant differ­rences in the enzyme activity of GPx values between groups.Conclusion: This study showed that the extract could increase the activity of GPx hepatic enzyme­es mice significantly.Keywords: physical exercise maximum, free radicals, purple sweet potato, anthocyanin, GPxCorrespondence: Ayu Elvana. School of Health Sciences (STIKes) Siti Hajar, Medan, Indonesia.Indonesian Journal of Medicine (2016), 1(2): 116-120https://doi.org/10.26911/theijmed.2016.01.02.0

Effect of Purple Sweet Potato (Ipomoea Batatas L.) on Reducing Renal Tissue Damage of House Mice (Mus Musculus L.) After Excessive Physical Exercise

Background: Heavy physical exercise can reduce blood flow and metabolism in kidney that eventually release free radicals. The free radicals can form oxydative stress and damage renal tissue. Exogenous antioxydant administration is usually recommended to minimize the renal tissue damage. This study aimed to examined the effect of purple sweet potato (Ipomoiea batatas L.) extract on renal tissue damage in mice (mus musculus L.) after heavy physical exercise. Subjects and Method: This was a randomized controlled trial with post test only control design. The study subjects included twenty four male white mice with DD Webster strain. These mice were divided into six groups. After undergoing excessive swimming exercises that lasted forteen days, purple sweet potato extract was given to the experimental group. There were three experimental groups receiving three different doses of purple sweet potato. The mice kidney was taken as sample for microscopic examination to determine the extent of tissue damage. Difference in renal tissue damage was tested by Kruskal-Wallis. Results: Microscopic examination showed statistically significant difference in tissue damage both in right (p=0.001) and left (p=0.036) kidneys, between study groups. The experimental groups showed less damaged than control group. Conclusion: Purple sweet potato (Ipomoiea batatas L) can lessen renal damage in male white mice (Mus musculus L) undergoing excessive physical exercise. Keywords: purple sweet potato (Ipomoiea batatas L), renal tissue damage mice Corespondance: Rika Nailuvar Sinaga. Faculty of Sport Science, Medan University. Email: [email protected]

Effect of Purple Sweet Potato (Ipomoea Batatas L.) Extract on Glutathione Peroxidase (GPx) Activities in Hepatic House Mice (Mus musculus) After Maximum Physical Exercise

Background: Physical exercise can improve antioxidant defense system of organis. But long and heavy exercise can disrupt the balance of oxidant-antioxidant. Low glutathione peroxidase is associated with free radicals. Plant purple sweet potatoes (Ipomoea batatas L.) contain anthocyanins high enough that act as antioxidants. This study aimed to determine the effect of extracts of tubers of purple sweet potato (Ipomoea Batatas L.) on the activity of glutathione peroxidase (GPx) liver of mice (Mus Musculus) treated with maximal physical exercise. Subjects and Method: This was a true experimental study. This conducted with the design of the control group post-test only in vivo on 24 white male mice (Mus musculus), DD Webster strain. Data analysis was using SPSS software 19. Statistical testing decision taken 5% significance level (p=0.05). Results: The results showed that the average enzyme activity of GPx in the P5 is the highest enzyme activity GPx (mean=19:39 ± SD=7:06, p=0.024), which means there are significant differrences in the enzyme activity of GPx values between groups. Conclusion: This study showed that the extract could increase the activity of GPx hepatic enzymees mice significantly. Keywords: physical exercise maximum, free radicals, purple sweet potato, anthocyanin, GPx Correspondence: Ayu Elvana. School of Health Sciences (STIKes) Siti Hajar, Medan, Indonesi