2 research outputs found
Hierarchical Micro- and Nanopatterning of Metallic Glass to Engineer Cellular Responses
Nano and micropatterning of biomaterials
is a rapidly evolving
technology used in the engineering sciences to control cell behavior.
Specifically, altering the topographies and hence surface mechanical
properties has been shown to induce changes in cell morphology and
function. Here, we show a method for fabricating hierarchical micro-
and nanopatterns of Pt<sub>57.5</sub>Cu<sub>14.7</sub>Ni<sub>5.3</sub>P<sub>22.5</sub> (Pt-BMG) on the relevant length scales comparable
to that of proteins and cells. Leveraging the amorphous nature
of Pt-BMGs, we have a versatile toolbox to manipulate patterns on
the nano/micro level and combine multiple length scales to examine
specific cell responses. We assay the morphology of macrophages and
fibroblasts, two cell types critical to the foreign body response.
Furthermore, we show that nanotopography is critical for reducing
macrophage fusion and that high levels of fusion on both unpatterned
and micropatterned substrates can be mitigated with the addition of
nanotopographical features. Interestingly, we show that the wetting
ability of the substrates does not correlate with cellular responses
on these substrates. Our results suggest that the different topographical
length scales can be used to systematically affect corresponding cell-type-specific
responses