3 research outputs found
Selection of antimicrobial frog peptides and temporin-1DRa analogues for treatment of bacterial infections based on their cytotoxicity and differential activity against pathogens
Selection of antimicrobial frog peptides and temporin-1DRa analogues for treatment of bacterial infections based on their cytotoxicity and differential activity against pathogens
Cationic, amphipathic, αâhelical hostâdefense peptides (HDPs) that are naturally secreted by certain species of frogs (Anura) possess potent broadâspectrum antimicrobial activity and show therapeutic potential as alternatives to treat infections by multiâdrug resistant pathogens. Fourteen amphibian skin peptides and twelve analogues of temporinâ1DRa were studied for their antimicrobial activities against clinically relevant human or animal skin infectionâassociated pathogens. For comparison, antimicrobial potencies of frog skin peptides against a range of probiotic lactobacilli were determined. We used the VITEK 2 system to define a profile of antibiotic susceptibility for the bacterial panel. The minimal inhibitory concentration (MIC) values of the naturally occurring temporinâ1DRa, CPFâAM1, alyteserinâ1c, hymenochirinâ2B, and hymenochirinâ4B for pathogenic bacteria were 3â to 9âfold lower than the values for the tested probiotic strains. Similarly, temporinâ1DRa and its [Lys4], [Lys5] and [Aib8] analogues showed 5â to 6.5âfold greater potency against the pathogens. In the case of PGLaâAM1, XTâ7, temporinâ1DRa and its [DâLys8] and [Aib13] analogues, no apoptosis or necrosis was detected in human peripheral blood mononuclear cells at concentrations below or above the MIC. Given the differential activity against commensal bacteria and pathogens, some of these peptides are promising candidates for further development into therapeutics for topical treatment of skin infection