CARD10 (caspase recruitment domain family, member 10)

Review on CARD10 (caspase recruitment domain family, member 10) , with data on DNA, on the protein encoded, and where the gene is implicated

Examination of Factors Related to Dental Anxiety Level in 1st Grade Students in Health High School of Namık Kemal University Vocational School of Health Services

Aim:Fear from dentists and dental treatments is common in society. The aim of this study is to investigate the level of dental anxiety, continuity and state anxiety, comparison and affecting factors in the 1st grade students of the Vocational School of Health Services (NOS) of Namik Kemal University Vocational School of Health Services.Materials and Methods:It is a cross-sectional study. The universe of the study is composed of the first year students of SHMYO / SYO (N = 362). The study reached 319 students (88%). Three questionnaires were applied to the students who volunteered to participate in the study. In the study, the relationship between the Dental Anxiety Score, Continuous and Instantaneous Anxiety Scores, Scale scores, and scores of scale scores were investigated and predicted anxiety prevalence rates were obtained.Results:As a result of the study, there was a significant relationship between DMAS and genders (p = 0.001). There was no relationship between tooth brushing frequency and MDAS (p = 0.253). There was a correlation between the frequency of going to the dentist and MDAS (p = 0.003). Recommendations of family and friends have a significant impact on the choice of individuals. There was a negative correlation between the MDI value and the TXI and TXII values in the pearson correlation analysis. It did not reach enough significance. There was no statistically significant correlation between MDAS and TXII values (r = 0,054).Conclusions:Women have higher dental concerns than men. This may be due to the patriarchal understanding common in society, because males are unable to express emotions that may show themselves weaker than women. In the light of these results, it was concluded that dental anxiety was independent of sociodemographic characteristics and state-trait anxiety

Effects of gestational exercise on hyperoxia-induced brain damage in the newborn

Aim: Preterm infants encounter hyperoxia relatively early on as they leave the intrauterine environment earlier than expected, while also being exposed to a higher level of hyperoxic stress due to insufficiencies in their antioxidant defense mechanisms. With that in mind, we investigate whether running exercises performed during pregnancy can contribute to the development of tolerance to neonatal hyperoxic brain damage. Method: While two female rats maintained a sedentary pregnancy, one female rat performed the mandatory running exercise for 30 minutes for five days a week throughout the pregnancy. Following delivery, the sedentary rats and the exercised rat were kept together with their offspring for five days at oxygen concentrations above 80 percent in order to induce brain damage. The offspring were sacrificed on postnatal Day 7 and brain/body ratio measurements were obtained. Results: The brain/body ratios in the control, hyperoxia and exercise-hyperoxia groups were found to be median (IQR) 0.074(0.68-0.77), 0.065(0.06-0.067) and 0.064(0.060-0.068), respectively. The brain/body ratios of the offspring of the mothers in the hyperoxia group were found to be significantly lower than the control group (p=0.002), irrespective of exercise (p=0.007). No statistically significant difference was noted between the offspring of the sedentary and the exercised mothers in the hyperoxia group (p=0.94). Conclusion: Hyperoxia was found to result in lower brain mass relative to total body mass. This finding, which indicates the presence of microcephaly, reflects the negative effects of hyperoxia on brain development. Contrary to expectations, exercises performed during pregnancy had no significant effect on the brain/body weight ratio of the offspring

A CpG island promoter drives the CXXC5 gene expression

CXXC5 is a member of the zinc-finger CXXC family that binds to unmethylated CpG dinucleotides. CXXC5 modulates gene expressions resulting in diverse cellular events mediated by distinct signaling pathways. However, the mechanism responsible for CXXC5 expression remains largely unknown. We found here that of the 14 annotated CXXC5 transcripts with distinct 5 ' untranslated regions encoding the same protein, transcript variant 2 with the highest expression level among variants represents the main transcript in cell models. The DNA segment in and at the immediate 5 '-sequences of the first exon of variant 2 contains a core promoter within which multiple transcription start sites are present. Residing in a region with high G-C nucleotide content and CpG repeats, the core promoter is unmethylated, deficient in nucleosomes, and associated with active RNA polymerase-II. These findings suggest that a CpG island promoter drives CXXC5 expression. Promoter pull-down revealed the association of various transcription factors (TFs) and transcription co-regulatory proteins, as well as proteins involved in histone/chromatin, DNA, and RNA processing with the core promoter. Of the TFs, we verified that ELF1 and MAZ contribute to CXXC5 expression. Moreover, the first exon of variant 2 may contain a G-quadruplex forming region that could modulate CXXC5 expression

The association of the amniotic fluid index (AFI) with perinatal fetal and maternal outcomes in pregnancies complicated by preterm premature rupture of membranes (PPROM)

Objectives: To investigate association of amniotic fluid index (AFI) with perinatal fetal and maternal outcomes in pregnancies complicated by preterm premature rupture of membranes (PPROM) Material and methods: A total of 70 singleton pregnancies complicated by PPROM at 23–33 weeks’ gestation were enrolled in this prospective observational study. Data on maternal clinical and obstetric characteristics [maternal age, gravidity, parity, PPROM time, and AFI (cm), latency period, treatments, type of delivery, length of hospital stay (LOS, day)], fetal characteristics (gestational age at delivery, birth weight (g), gender) and maternal and fetal complications were recorded and compared in AFI < 5 cm (n = 27) and AFI ≥ 5 cm (n = 21) groups. Results: Overall AFI was ≤ 5 cm in 27 (56.3%) patients and > 5 cm in 21 (43.7%) patients. No significant difference was noted in maternal clinical and obstetric characteristics, gestational age at delivery and gender of the newborn as well as in maternal and fetal complications rates with respect to AFI groups. AFI was correlated positively with latency period (r = 0.399, p = 0.018) and negatively with postpartum LOS (r = –0.314, p = 0.030). Conclusions: In conclusion, our findings seems to indicate increased likelihood of shorter latency to delivery and longer postpartum LOS with decrease in AFI after PPROM between 23–33 weeks’ gestation, whereas no impact of AFI on mode of delivery and fetal or maternal complications

CXXC-tipi çinko parmak 5 proteinin (CXXC5) yapısal ve fonksiyonel karakterizasyonu.

Estrogen hormones, particularly 17β-estradiol (E2), are involved in the regulation of physiological and pathophysiological functions of many organs and tissues including breast tissue. The expression of CXXC type zinc finger protein 5 (CXXC5) gene is regulated by E2 through estrogen receptor α. Due to a highly conserved zinc-finger CXXC domain (ZF-CXXC), CXXC5 is considered to be a member of ZF-CXXC family, which binds to non-methylated CpG dinucleotides of transcriptionally active DNA regions. This binding is thought to play critical roles in epigenetic modulation of transcription through the prevention of cytosine methylation and the recruitment of DNA modifying enzymes. The structure and function of CXXC5 and its role in cellular events are yet unclear. However, accumulating evidence is suggesting that CXXC5 is involved in transcriptions as a transcription factor, co-regulator and/or epigenetic factor. In this PhD thesis, I successfully expressed and purified the full-length CXXC5 protein, with which I showed that CXXC5 is a non-methylated CpG DNA binding protein and that the ZF-CXXC domain is indeed responsible for the ability of the protein to interact with DNA. Since proteins exert their functions in the context of dynamically changing interacting protein network, I envisioned that identification of interacting protein partners of CXXC5 would be a critical step in the elucidation of cellular function of the protein. To address this issue, I performed proximity dependent biotinylation assay (BioID) in a cell line model derived from breast adenocarcinoma. Of the identified proteins by Liquid chromatography-tandem mass spectrometry (LC-MS/MS), I validated that CXXC5 protein interacts with MeCP2 (MethylCpG binding protein 2), MAZ (Myc-associated Zinc Finger Protein) and EMD (Emerin) proteins by co-immunoprecipitation assay. I found that the zinc finger domain of CXXC5 is necessary for protein interaction as well. The findings of this study could provide important insights into the mechanism of CXXC5 actions in E2- mediated cellular events.Ph.D. - Doctoral Progra

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

CXXC-TİPİ ÇİNKO PARMAK 5 PROTEİNİNİN YAPISAL VE FONKSİYONEL KARAKTERİZASYON

Examination of Factors Related to Dental Anxiety Level in 1st Grade Students in Health High School of Namık Kemal University Vocational School of Health Services

Amaç: Diş hekimi ve diş tedavilerinden korku toplumda yaygın görülen bir durumdur. Bu çalışmanın amacı Namık Kemal Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu (SHMYO) Sağlık Yüksek Okulu (SYO) 1. Sınıf öğrencilerinde dental anksiyete düzeyi, süreklilik ve durumluluk kaygı düzeyi, kıyaslanması ve etkileyen faktörlerin incelenmesidir. Materyal ve Metot: Kesitsel bir araştırmadır. Araştırmanın evrenini SHMYO/SYO 1. sınıf öğrencileri oluşturmaktadır (N=362). Çalışmada 319 öğrenciye ulaşılmıştır (%88). Araştırmaya katılmaya gönüllü olan öğrencilere üç soru formu uygulanmıştır. Araştırmada Dental Anksiyete Skoru, Sürekli ve Anlık Anksiyete skorları, Ölçek skorlarının birbirleri ile ilişkisi ve elde edilen ölçek skorlarının kimi bağımsız değişkenlere göre değişimi incelenmiş ve tahmini anksiyete prevalans hızları elde edilmiştir. Bulgular: Çalışma sonucunda DMAS ile cinsiyetler arasında anlamlı bir ilişki vardır (p=0.001). Kadınların MDAS’sı erkeklerden daha yüksektir. Diş fırçalama sıklığı ile MDAS arasında bir ilişki yoktur (p=0,253).. Diş hekimine gitme sıklığı ile MDAS arasında ilişki vardır (p=0,003). Aile ve arkadaşların tavsiyeleri kişilerin seçimini belirgin bir şekilde etkilemektedir. MDAS değeri ile TXI ve TXII değerleri arasında bakılan pearson korelasyon analizinde TXI ile MDAS değerleri arasında negatif korelasyon bulunmuş olup istatistiksel olarak anlamsızdır (r=-0,087). Yeterli anlamlılığa erişmemiştir. MDAS TXII değerleri arasında ise istatistiksel olarak anlamlı olmayan pozitif korelasyon bulunmuştur (r=0,054). Sonuç: Kadınların dental kaygıları erkeklerden daha yüksektir. Bu durum toplumda yaygın olan ataerkil anlayış nedeniyle erkeklerin kadınlara göre kendilerini daha zayıf gösterebilecek duygu durumları açıkça ifade edememelerinden kaynaklanabilir. Bu sonuçlar ışığında dental anksiyetenin sosyodemografik özelliklerden ve kişinin durumluluk-süreklilik anksiyetesinden de bağımsız olduğu kanısına varılmıştır.Aim: Fear from dentists and dental treatments is common in society. The aim of this study is to investigate the level of dental anxiety, continuity and state anxiety, comparison and affecting factors in the 1st grade students of the Vocational School of Health Services (NOS) of Namik Kemal University Vocational School of Health Services. Materials and Methods: It is a cross-sectional study. The universe of the study is composed of the first year students of SHMYO / SYO (N = 362). The study reached 319 students (88%). Three questionnaires were applied to the students who volunteered to participate in the study. In the study, the relationship between the Dental Anxiety Score, Continuous and Instantaneous Anxiety Scores, Scale scores, and scores of scale scores were investigated and predicted anxiety prevalence rates were obtained. Results: As a result of the study, there was a significant relationship between DMAS and genders (p = 0.001). There was no relationship between tooth brushing frequency and MDAS (p = 0.253). There was a correlation between the frequency of going to the dentist and MDAS (p = 0.003). Recommendations of family and friends have a significant impact on the choice of individuals. There was a negative correlation between the MDI value and the TXI and TXII values in the pearson correlation analysis. It did not reach enough significance. There was no statistically significant correlation between MDAS and TXII values (r = 0,054). Conclusions: Women have higher dental concerns than men. This may be due to the patriarchal understanding common in society, because males are unable to express emotions that may show themselves weaker than women. In the light of these results, it was concluded that dental anxiety was independent of sociodemographic characteristics and state-trait anxiety

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

ÖSTROJEN SİNYALİ İLE DÜZENLENEN MOAP1 GENİNİN MEME KANSERİ HÜCRE HATLARINDAKİ APOPTOTİK ROL

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

ÖSTROJEN SİNYALİ İLE DÜZENLENEN MOAP1 GENİNİN MEME KANSERİ HÜCRE HATLARINDAKİ APOPTOTİK ROL