4 research outputs found
A New Lysosome-Targetable Turn-On Fluorogenic Probe for Carbon Monoxide Imaging in Living Cells
A lysosome-targetable
fluorogenic probe, LysoFP-NO<sub>2</sub>,
was designed and synthesized based on a naphthalimide fluorophore
that can detect selectively carbon monoxide (CO) in HEPES buffer (pH
7.4, 37 Ā°C) through the transformation of the nitro group into
an amino-functionalized system in the presence of CO. LysoFP-NO<sub>2</sub> triggered a āturn-onā fluorescence response
to CO with a simultaneous increase of fluorescence intensity by more
than 75 times. The response is selective over a variety of relevant
reactive nitrogen, oxygen, and sulfur species. Also, the probe is
an efficient candidate for monitoring changes in intracellular CO
in living cells (MCF7), and the fluorescence signals specifically
localize in the lysosome compartment
Synthesis, Structure, Spectroscopic Characterization, and Protein Binding Affinity of New Water-Soluble Hetero- and Homometallic Tetranuclear [Cu<sup>II</sup><sub>2</sub>Zn<sup>II</sup><sub>2</sub>] and [Cu<sup>II</sup><sub>4</sub>] Clusters
Two
new water-soluble hetero- and homometallic tetranuclear clusters,
Na<sub>4</sub>[Cu<sub>2</sub>Zn<sub>2</sub>(ccdp)<sub>2</sub>(Ī¼-OH)<sub>2</sub>]Ā·CH<sub>3</sub>OHĀ·6H<sub>2</sub>O (<b>1</b>) and K<sub>3</sub>[Cu<sub>4</sub>(ccdp)<sub>2</sub>(Ī¼-OH)Ā(Ī¼-OH<sub>2</sub>)]Ā·14H<sub>2</sub>O (<b>2</b>), have been
synthesized in methanolāwater at room temperature by exploiting
the flexibility, chelating ability,
and bridging potential of a carboxylate-rich dinucleating ligand, <i>N</i>,<i>N</i>ā²-bisĀ(2-carboxybenzomethyl)-<i>N</i>,<i>N</i>ā²-bisĀ(carboxymethyl)-1,3 diaminopropan-2-ol
(H<sub>5</sub>ccdp). Complex <b>1</b> is obtained through the
self-assembly of two monoanionic [CuZnĀ(ccdp)]<sup>ā</sup> fragments,
which are, in turn, exclusively bridged by two Ī¼-OH<sup>ā</sup> groups. Similarly, complex <b>2</b> is formed through
the self-assembly of two monoanionic [Cu<sub>2</sub>(ccdp)]<sup>ā</sup> species exclusively bridged by one Ī¼-OH<sup>ā</sup> and one Ī¼-OH<sub>2</sub> groups. Complexes <b>1</b> and <b>2</b> are
fully characterized in the solid state as well as in solution using
various analytical techniques including a single-crystal X-ray diffraction
study. The X-ray crystal structure of <b>1</b> reveals that
two Cu<sup>II</sup> centers are in a distorted square-pyramidal geometry,
whereas two
Zn<sup>II</sup> centers are in a distorted trigonal-bipyramidal geometry.
The solid-state structure of <b>2</b> contains two dinuclear
[Cu<sub>2</sub>(ccdp)]<sup>ā</sup> units having one Cu<sup>II</sup> center in a distorted square-pyramidal
geometry and another Cu<sup>II</sup> center in a distorted trigonal-bipyramidal
geometry within each dinuclear unit. In the powder state, the high-field
EPR spectrum of complex <b>1</b> indicates that two Cu<sup>II</sup> ions are not spin-coupled, whereas that of complex <b>2</b> exhibits at least one noninteracting Cu<sup>II</sup> center coordinated
to a nitrogen atom of the ligand.
Both complexes are investigated for their binding affinity with the
protein bovine serum albumin (BSA) in an aqueous medium at pH ā¼7.2
using fluorescence spectroscopy. Synchronous fluorescence spectra
clearly reveal that complexes <b>1</b> and <b>2</b> bind
to the active sites in the protein, indicating that the effect is
more pronounced toward tyrosine than tryptophan. Density functional
theory calculations
have been carried to find the Fukui functions at the metal sites in
complexes <b>1</b> and <b>2</b> to predict the possible
metal centers involved in the binding process with BSA protein
Synthesis, Structure, Spectroscopic Characterization, and Protein Binding Affinity of New Water-Soluble Hetero- and Homometallic Tetranuclear [Cu<sup>II</sup><sub>2</sub>Zn<sup>II</sup><sub>2</sub>] and [Cu<sup>II</sup><sub>4</sub>] Clusters
Two
new water-soluble hetero- and homometallic tetranuclear clusters,
Na<sub>4</sub>[Cu<sub>2</sub>Zn<sub>2</sub>(ccdp)<sub>2</sub>(Ī¼-OH)<sub>2</sub>]Ā·CH<sub>3</sub>OHĀ·6H<sub>2</sub>O (<b>1</b>) and K<sub>3</sub>[Cu<sub>4</sub>(ccdp)<sub>2</sub>(Ī¼-OH)Ā(Ī¼-OH<sub>2</sub>)]Ā·14H<sub>2</sub>O (<b>2</b>), have been
synthesized in methanolāwater at room temperature by exploiting
the flexibility, chelating ability,
and bridging potential of a carboxylate-rich dinucleating ligand, <i>N</i>,<i>N</i>ā²-bisĀ(2-carboxybenzomethyl)-<i>N</i>,<i>N</i>ā²-bisĀ(carboxymethyl)-1,3 diaminopropan-2-ol
(H<sub>5</sub>ccdp). Complex <b>1</b> is obtained through the
self-assembly of two monoanionic [CuZnĀ(ccdp)]<sup>ā</sup> fragments,
which are, in turn, exclusively bridged by two Ī¼-OH<sup>ā</sup> groups. Similarly, complex <b>2</b> is formed through
the self-assembly of two monoanionic [Cu<sub>2</sub>(ccdp)]<sup>ā</sup> species exclusively bridged by one Ī¼-OH<sup>ā</sup> and one Ī¼-OH<sub>2</sub> groups. Complexes <b>1</b> and <b>2</b> are
fully characterized in the solid state as well as in solution using
various analytical techniques including a single-crystal X-ray diffraction
study. The X-ray crystal structure of <b>1</b> reveals that
two Cu<sup>II</sup> centers are in a distorted square-pyramidal geometry,
whereas two
Zn<sup>II</sup> centers are in a distorted trigonal-bipyramidal geometry.
The solid-state structure of <b>2</b> contains two dinuclear
[Cu<sub>2</sub>(ccdp)]<sup>ā</sup> units having one Cu<sup>II</sup> center in a distorted square-pyramidal
geometry and another Cu<sup>II</sup> center in a distorted trigonal-bipyramidal
geometry within each dinuclear unit. In the powder state, the high-field
EPR spectrum of complex <b>1</b> indicates that two Cu<sup>II</sup> ions are not spin-coupled, whereas that of complex <b>2</b> exhibits at least one noninteracting Cu<sup>II</sup> center coordinated
to a nitrogen atom of the ligand.
Both complexes are investigated for their binding affinity with the
protein bovine serum albumin (BSA) in an aqueous medium at pH ā¼7.2
using fluorescence spectroscopy. Synchronous fluorescence spectra
clearly reveal that complexes <b>1</b> and <b>2</b> bind
to the active sites in the protein, indicating that the effect is
more pronounced toward tyrosine than tryptophan. Density functional
theory calculations
have been carried to find the Fukui functions at the metal sites in
complexes <b>1</b> and <b>2</b> to predict the possible
metal centers involved in the binding process with BSA protein
Synthesis, Structure, Spectroscopic Characterization, and Protein Binding Affinity of New Water-Soluble Hetero- and Homometallic Tetranuclear [Cu<sup>II</sup><sub>2</sub>Zn<sup>II</sup><sub>2</sub>] and [Cu<sup>II</sup><sub>4</sub>] Clusters
Two
new water-soluble hetero- and homometallic tetranuclear clusters,
Na<sub>4</sub>[Cu<sub>2</sub>Zn<sub>2</sub>(ccdp)<sub>2</sub>(Ī¼-OH)<sub>2</sub>]Ā·CH<sub>3</sub>OHĀ·6H<sub>2</sub>O (<b>1</b>) and K<sub>3</sub>[Cu<sub>4</sub>(ccdp)<sub>2</sub>(Ī¼-OH)Ā(Ī¼-OH<sub>2</sub>)]Ā·14H<sub>2</sub>O (<b>2</b>), have been
synthesized in methanolāwater at room temperature by exploiting
the flexibility, chelating ability,
and bridging potential of a carboxylate-rich dinucleating ligand, <i>N</i>,<i>N</i>ā²-bisĀ(2-carboxybenzomethyl)-<i>N</i>,<i>N</i>ā²-bisĀ(carboxymethyl)-1,3 diaminopropan-2-ol
(H<sub>5</sub>ccdp). Complex <b>1</b> is obtained through the
self-assembly of two monoanionic [CuZnĀ(ccdp)]<sup>ā</sup> fragments,
which are, in turn, exclusively bridged by two Ī¼-OH<sup>ā</sup> groups. Similarly, complex <b>2</b> is formed through
the self-assembly of two monoanionic [Cu<sub>2</sub>(ccdp)]<sup>ā</sup> species exclusively bridged by one Ī¼-OH<sup>ā</sup> and one Ī¼-OH<sub>2</sub> groups. Complexes <b>1</b> and <b>2</b> are
fully characterized in the solid state as well as in solution using
various analytical techniques including a single-crystal X-ray diffraction
study. The X-ray crystal structure of <b>1</b> reveals that
two Cu<sup>II</sup> centers are in a distorted square-pyramidal geometry,
whereas two
Zn<sup>II</sup> centers are in a distorted trigonal-bipyramidal geometry.
The solid-state structure of <b>2</b> contains two dinuclear
[Cu<sub>2</sub>(ccdp)]<sup>ā</sup> units having one Cu<sup>II</sup> center in a distorted square-pyramidal
geometry and another Cu<sup>II</sup> center in a distorted trigonal-bipyramidal
geometry within each dinuclear unit. In the powder state, the high-field
EPR spectrum of complex <b>1</b> indicates that two Cu<sup>II</sup> ions are not spin-coupled, whereas that of complex <b>2</b> exhibits at least one noninteracting Cu<sup>II</sup> center coordinated
to a nitrogen atom of the ligand.
Both complexes are investigated for their binding affinity with the
protein bovine serum albumin (BSA) in an aqueous medium at pH ā¼7.2
using fluorescence spectroscopy. Synchronous fluorescence spectra
clearly reveal that complexes <b>1</b> and <b>2</b> bind
to the active sites in the protein, indicating that the effect is
more pronounced toward tyrosine than tryptophan. Density functional
theory calculations
have been carried to find the Fukui functions at the metal sites in
complexes <b>1</b> and <b>2</b> to predict the possible
metal centers involved in the binding process with BSA protein