A rare localization of tuberculosis under infliximab treatment: Testicular involvement

Infliximab is an inhibitory of tumor necrosis factor-alpha which is used successfully for the treatment of inflammatory bowel disease and rheumatic disease. It has various side effects including injection-induced reactions, immunosuppression, demyelinating diseases, and cardiac effects. One of the most serious side effects is tuberculosis. In particular, the immunosuppressant drugs have a high risk of reactivating latent tuberculosis infection. Its activation probably may occur as an extra-pulmonary and, occasionally, may result in an unusual infection. Herein, we report a 30-year-old male case treated with infliximab and suffered from isolated testicular tuberculosis

α-Chaconine and α-Solanine Inhibit RL95-2 Endometrium Cancer Cell Proliferation by Reducing Expression of Akt (Ser473) and ERα (Ser167)

The aim of this study is to investigate the potential inhibitory effect of α-chaconine and α-solanine on RL95-2 estrogen receptor (ER) positive human endometrial cancer cell line and to identify the effect of these glycoalkaloids on the Akt signaling and ERα. The cell proliferation profiles and the cytotoxicity studies were performed by Real-Time Cell Analyzer (xCELLigence) and compared with Sulphorhodamine B (SRB) assay. The effects of α-chaconine (2.5, 5, 10 µM), α-solanine (20, 30, 50 µM), API-1 (25 µM) and MPP (20 µM) effects on Akt (Ser473) and ERα (Ser167) expressions evaluated by Western blot and qPCR method. Their IC50 values were as α-chaconine (4.72 µM) < MPP (20.01 µM) < α-solanine (26.27 µM) < API-1 (56.67 µM). 10 μM α-chaconine and 20, 30 and 50 μM α-solanine were effective in decreasing p-Akt(Ser473)/Akt ratio compared to positive control API-1. When the p-ERα/ERα ratios were evaluated, it was observed that α-chaconine (2.5, 5, 10 μM) and α-solanine (50 μM) were as effective as the specific ERα inhibitor MPP in reducing the ratio of p-ERα/ERα compared to the control group. In conclusion, it has been shown that the proliferation of α-chaconine and α-solanine in human endometrial carcinoma cells reduces the expression and activity of the Akt and ERα signaling pathway

Neuroprotective effect of Capparis ovata Desf. var. palaestina Zoh. on H2O2induced neurotoxicity in SH-SY5Y cells Efecto neuroprotector de Capparis ovata Desf. var. palaestina Zoh. sobre la neurotoxicidad inducida por H2O2 en células SH - SY5Y

The neuroprotective effect of flower and fruit parts of Capparis ovata Desf. var. palaestina Zoh. plant was investigated in H2O2-induced cytotoxicity in SH-SY5Y cells. The cells were treated with H2O2 alone or pretreated with flower (COMFL) and fruit extract (COMFR) of C. ovata var. palaestina. MTT, xCELLigence, and qualitative and quantitative determination of phytochemical constituents in the extracts by LC-MS/MS methods were employed. COMFL and COMFR had a neuroprotective effect and this effect was stronger when the presence of oxidative stress. The mass spectrums revealed the presence of flavonoids and phenolic acid derivatives in the extracts. According to quantitative analyses, the main compounds were myristoleic acid, apigenin, caffeic acid, caffeic acid-3-glucoside, and 5-cynapoil quinic acid in both COMFL and COMFR and rutin was found in COMFL. The extracts could inhibit H2O2 induced neuronal cell death which might be beneficial for the pretreatment of oxidative stress in neurodegenerative diseases

alpha-Chaconine and alpha-Solanine Inhibit RL95-2 Endometrium Cancer Cell Proliferation by Reducing Expression of Akt (Ser473) and ER alpha (Ser167)

The aim of this study is to investigate the potential inhibitory effect of alpha-chaconine and alpha-solanine on RL95-2 estrogen receptor (ER) positive human endometrial cancer cell line and to identify the effect of these glycoalkaloids on the Akt signaling and ER alpha. The cell proliferation profiles and the cytotoxicity studies were performed by Real-Time Cell Analyzer (xCELLigence) and compared with Sulphorhodamine B (SRB) assay. The effects of alpha-chaconine (2.5, 5, 10 mu M), alpha-solanine (20, 30, 50 mu M), API-1 (25 mu M) and MPP (20 mu M) effects on Akt (Ser473) and ER alpha (Ser167) expressions evaluated by Western blot and qPCR method. Their IC50 values were as alpha-chaconine (4.72 mu M) < MPP (20.01 mu M) < alpha-solanine (26.27 mu M) < API-1 (56.67 mu M). 10 mu M alpha-chaconine and 20, 30 and 50 mu M alpha-solanine were effective in decreasing p-Akt(Ser473)/Akt ratio compared to positive control API-1. When the p-ER alpha/ER alpha ratios were evaluated, it was observed that alpha-chaconine (2.5, 5, 10 mu M) and alpha-solanine (50 mu M) were as effective as the specific ER alpha inhibitor MPP in reducing the ratio of p-ER alpha/ER alpha compared to the control group. In conclusion, it has been shown that the proliferation of alpha-chaconine and alpha-solanine in human endometrial carcinoma cells reduces the expression and activity of the Akt and ER alpha signaling pathway