Assessment of Centella Asiatica Extract Containing Dual-crosslinked Gel-MA/Pec Hydrogels as Wound Dressing

In the present work, 3D-printed wound dressings containing different amounts of Centella Asiatica extract were synthesized via dual-crosslinking method. Methacrylic anhydride modified gelatin (Gel-MA) and pectin (Pec) were chosen as the base material for dressings. A dual crosslinked network was formed with Gel-MA photo-crosslinking using Irgacure 2959 and Pectin (Pec) psychical-crosslinking using Ca++ ions. Meanwhile, the developed dual-crosslinked hydrogel dressings were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and mechanical, swelling, in vitro degradation as well as ex vivo bioadhesion tests. It has been observed that the preapred dressings provide good bioadhesion on the chicken skin. Moreover, MTT assay revealed cell viability of Gel-MA/Pec hydrogel dressings containing Centella Asiatica extract. The results presented the dressings as a promising biomaterial to be used for wound healing purposes, which should be further investigated in future

Preparation of quercetin incorporated photocrosslinkable methacrylated gelatin/methacrylated kappa-carrageenan antioxidant hydrogel wound dressings

© 2022, Institute of Chemistry, Slovak Academy of Sciences.Wound dressings with antioxidant potential have attracted remarkable concerns for wound healing. The purpose of this work is to investigate blank, and quercetin loaded photocrosslinkable methacrylated gelatin (GelMA)/methacrylated kappa-carrageenan (KapMA) hydrogel. Wound dressings were produced by the photocrosslinking method in the presence of a photoinitiator. Quercetin was incorporated in photocrosslinkable GelMA/KapMA wound dressings with different ratios. Besides, Fourier Transform Infrared Spectroscopy, thermal gravimetric analysis, swelling, and degradation tests were performed for characterization studies of the hydrogel wound dressings. The release of quercetin from GelMA/KapMA wound dressings was investigated at a physiological pH value of 7.4 at 37 °C. The antioxidant activity of the hydrogel dressings was further evaluated by 2,2-diphenyl-1-picrylhydrazyl method. Biocompatibility of wound dressings was assessed using MTT assay tests on NIH-3T3 cell line, which evaluated its suitability in biomaterial applications. The obtained results indicated that quercetin incorporated photocrosslinked GelMA/KapMA wound dressings provide antioxidant activity, and they could be the ability for the potential wound dressings applications

Photocurable silk fibroin-based tissue sealants with enhanced adhesive properties for the treatment of corneal perforations

Corneal defects are associated with corneal tissue engineering in terms of vision loss. The treatment of corneal defects is an important clinical challenge due to a uniform corneal thickness and the apparent lack of regenerative ability. In this work, we synthesized a biocompatible and photocrosslinkable ocular tissue adhesive composite hydrogel prepared by using methacrylated gelatin (GelMA), which is called the most favorable derivative of gelatin used as a tissue adhesive, silk fibroin (SF), and GelMA/SF (GS) with high adhesion behaviours for use in corneal injuries. The adhesion behaviours of the materials prepared in the presence of silk fibroin were improved. Importantly, the effect of different UV curing times on the adhesion properties of the prepared materials was also investigated. The prepared GS tissue adhesives showed high physiological adhesion. GS can be modulated to increase its adhesive strength up to 3 times compared to G. GS was also found to be biocompatible and have a high healing potential. In addition, the obtained transmission value of GS is also close to that of the human cornea. GS supported cellular adhesion and proliferation. The burst pressure strength for fresh cornea of the GS-60s sealants (144.5 ± 13 kPa) was determined to be higher than that of the G-60s sealants (52.6 ± 33.5 kPa)

The effect of thiol functional groups on bovine serum albumin/chitosan buccal mucoadhesive patches

In this research, the effect of thiol functional groups on bovine serum albumin (BSA)/chitosan (Chi) based buccal mucoadhesive patch was investigated. Thiolated BSA (BSA-SH) was prepared via 2-mercaptoethanol. FTIR and 1H NMR results confirmed that BSA-SH was synthesized successfully. The buccal mucoadhesive patches were fabricated by the solvent casting method. Following the structural characterization of BSA/Chi and BSA-SH/Chi buccal patches, the mechanical characterization was performed by tensile tests. The drug release from triamcinolone acetonide (TR) loaded buccal patches was evaluated in-vitro in simulated salivary. According to the ex-vivo buccal adhesion experiments, the mechanical and mucoadhesion properties of BSA-SH/Chi buccal patch had improved compared to BSA/Chi buccal patch. The total cumulative TR permeated after 12 h was higher for BSA-SH/Chi than BSA/Chi buccal patches. The developed mucoadhesive buccal patches were found to be biocompatible in vitro. To conclude, the thiolated BSA-SH/Chi buccal adhesive patch is a promising biomaterial for a satisfied drug delivery, which provides advantages for various oral applications

Aloe vera-based antibacterial porous sponges for wound dressing applications

The antibacterial sponges with high macroporosity, high interconnectivity and high biocompatibility is a significant concern for wound healing applications. In this work, novel Aloe vera (AV) based sponges were developed via subsequent lyophilization with further chemical crosslinking throughout the double network sponges. Single network was composed of gelatin-sodium alginate (G-SA) while the double network using gelatin-sodium alginate- sodium hyaluronate (G-SA-HA) was produced with the addition of hyaluronic acid solution into the gelatin-sodium alginate matrix. Lastly, Aloe vera as the bioactive agent was fabricated throughout the gelatin-sodium alginate-sodium hyaluronate matrix. The AV-based sponges demonstrated large pores with high interconnectivity. The swelling degree of the AV-based sponges were higher than that of G-SA and G-SA-HA sponges. The release of AV from the sponges reached an equilibrium value after 24 h showing a more controlled release at pH 5.5 than at pH 7.4. AV-based sponges showed antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Streptococcus pneumoniae and Bacillus cereus and displayed any cytotoxicity against Mesenchymal stem cells.Hitit University ; Hitit University Scientific and Tecnical Application and Research Cente

Development of mucoadhesive modified kappa-carrageenan/pectin patches for controlled delivery of drug in the buccal cavity

In this study, modified kappa-carrageenan/pectin hydrogel patches were fabricated for treatment of buccal fungal infections. For this purpose, kappa-carrageenan-g-acrylic acid was modified with different thiolated agents (L-cysteine and 3-mercaptopropionic acid), and the thiol content of the resulting modified kappa-carrageenan was confirmed by elemental analyzer. Then, the hydrogel patches were fabricated, and characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, ex vivo mucoadhesion test, and swelling behavior. Triamcinolone acetonide was added either directly or by encapsulating within the poly(lactic-co-glycolic acid) nanoparticles. The release amount of the drug from the directly loaded patch was 7.81 mg/g polymer, while it was 3.28 mg/g polymer for the encapsulated patch with the same content at 7 hr. The hydrogel patches had no cytotoxicity by cell culture studies. Finally, the drug loaded hydrogel patches were demonstrated antifungal activity against Aspergillus fumigatus and Aspergillus flavus. These results provide that the novel modified kappa-carrageenan and pectin based buccal delivery system has promising antifungal property, and could have advantages compared to conventional buccal delivery systems

Application of convergent science and technology toward ocular disease treatment

Eyes are one of the main critical organs of the body that provide our brain with the most information about the surrounding environment. Disturbance in the activity of this informational organ, resulting from different ocular diseases, could affect the quality of life, so finding appropriate methods for treating ocular disease has attracted lots of attention. This is especially due to the ineffectiveness of the conventional therapeutic method to deliver drugs into the interior parts of the eye, and the also presence of barriers such as tear film, blood-ocular, and blood-retina barriers. Recently, some novel techniques, such as different types of contact lenses, micro and nanoneedles and in situ gels, have been introduced which can overcome the previously mentioned barriers. These novel techniques could enhance the bioavailability of therapeutic components inside the eyes, deliver them to the posterior side of the eyes, release them in a controlled manner, and reduce the side effects of previous methods (such as eye drops). Accordingly, this review paper aims to summarize some of the evidence on the effectiveness of these new techniques for treating ocular disease, their preclinical and clinical progression, current limitations, and future perspectives

Selection of natural biomaterials for micro-tissue and organ-on-chip models

The desired organ in micro-tissue models of organ-on-a-chip (OoC) devices dictates the optimum biomaterials, divided into natural and synthetic biomaterials. They can resemble biological tissues' biological functions and architectures by constructing bioactivity of macromolecules, cells, nanoparticles, and other biological agents. The inclusion of such components in OoCs allows them having biological processes, such as basic biorecognition, enzymatic cleavage, and regulated drug release. In this report, we review natural-based biomaterials that are used in OoCs and their main characteristics. We address the preparation, modification, and characterization methods of natural-based biomaterials and summarize recent reports on their applications in the design and fabrication of micro-tissue models. This article will help bioengineers select the proper biomaterials based on developing new technologies to meet clinical expectations and improve patient outcomes fusing disease modeling