Effects of host defense peptides B2RP, Brevinin-2GU, D-Lys-Temporin, Lys-XT-7 and D-Lys-Ascaphin-8 on peripheral blood mononuclear cells: Preliminary study

Background and purpose: Host defense peptides have considerable therapeutic potential. One of the limitations for their therapeutic use is insufficient selectivity of some peptides, i.e. toxicity for eukaryotic cells. In this study, we have investigated effect of two naturally occurring and three analogs of frog skin-derived peptides on viability/proliferation of resting peripheral blood mononuclear cells and activated lymphocytes.Materials and Methods: Effect of tested peptides was assessed using MTT colorimetric assay. Concanavalin A was used as lymphocyte mitogen.Results: Brevinin-2GU induced cell death only in the highest tested concentration, whereas other peptides were not cytotoxic to resting peripheral blood mononuclear cells. Moreover, high concentrations of B2RP, D-Lys-Ascaphin-8  and Lys-XT-7 induced cell proliferation and this effect was more prominent in lymphocytes (pConclusions: Tested peptides (with exception of Brevinin-2GU) didn’t show cytotoxicity toward peripheral blood mononuclear cells. Moreover, they have potential to modulate immune response by inducing proliferation of resting peripheral blood mononuclear cells and limiting proliferative response to the activation stimulus. Regarding their potent antimicrobial and low hemolytic activity this makes them good candidates for therapeutic use.Key words: host defence peptides; cytotoxicity; immunomodulation </p

Effects of host defense peptides B2RP, Brevinin-2GU, D-Lys-Temporin, Lys-XT-7 and D-Lys-Ascaphin-8 on peripheral blood mononuclear cells: Preliminary study

© 2017 Croatian Society of Natural Sciences. All rights reserved. Background and purpose: Host defense peptides have considerable therapeutic potential. One of the limitations for their therapeutic use is insufficient selectivity of some peptides, i.e. toxicity for eukaryotic cells. In this study, we have investigated effect of two naturally occurring and three analogs of frog skin-derived peptides on viability/proliferation of resting peripheral blood mononuclear cells and activated lymphocytes. Materials and Methods: Effect of tested peptides was assessed using MTT colorimetric assay. Concanavalin A was used as lymphocyte mitogen. Results: Brevinin-2GU induced cell death only in the highest tested concentration, whereas other peptides were not cytotoxic to resting peripheral blood mononuclear cells. Moreover, high concentrations of B2RP, DLys-Ascaphin-8 and Lys-XT-7 induced cell proliferation and this effect was more prominent in lymphocytes (p<0.05). Tested peptides had opposite effect on activated lymphocytes inhibiting proliferative response to Concanavalin A (Brevinin-2GU, B2RP and D-Lys-Temporin p<0.05). Conclusions: Tested peptides (with exception of Brevinin-2GU) didn’t show cytotoxicity toward peripheral blood mononuclear cells. Moreover, they have potential to modulate immune response by inducing proliferation of resting peripheral blood mononuclear cells and limiting proliferative response to the activation stimulus. Regarding their potent antimicrobial and low hemolytic activity this makes them good candidates for therapeutic use

Politique internationale

SYL-001456 = Fascicule 1, partie 1 ;SYL-8489 = Fascicule 1, partie 2 ;SYL-001457 = Fascicule 2, partie 1 ;SYL-001458 = Fascicule 2, partie 2 ;SYL-001459 = Fascicule 3 ;SYL-001460 = Fascicule 4/5 ;SYL-001461 = Fascicule 6 ;SYL-001811 = Fascicule 7 ;SYL-001462 = Fascicule 8 ;SYL-001812 = Fascicule 9Fascicule 1, partie 1 :World Politics / Pedrag Avramovic, avec l'aide de F. Misrahi et E. Philippart -- Fascicule 1, partie 2 :World Politics / Pedrag Avramovic, avec l'aide de F. Misrahi et E. Philippart -- Fascicule 2, partie 1 :World Economy / Pedrag Avramovic avec l'aide de E. Külahci, E. Laurent -- Fascicule 2, partie 2 :World Economy / Pedrag Avramovic avec l'aide de E. Külahci, E. Laurent -- Fascicule 3 :Security issues and peace studies / Pedrag Avramovic, avec l'aide de Séverine Cirlande et Alexandra Lainé -- Fascicule 4/5 :USA and CIS / Pedrag Avramovic avec l'aide de Séverine Cirlande et Alexandra Lainé -- Fascicule 6 :Europe / Pedrag Avramovic, Erol Külahci -- Fascicule 7 :Maghreb & Middle East / S. Cirlande, E. Laurent -- Fascicule 8 :Asia & Pacific Basin -- Fascicule 9 :Latin America / Erol Külahci & Alexandra LainéSyllabus strictement réservé aux étudiants suivant les cours dans le cadre du CERIS - EXTE 000info:eu-repo/semantics/published

The cytotoxicity of korbazol against murine cancer cell lines

Background/aim: Korbazol is a natural product that has been shown to exert cytotoxic eff ects on leukemic cells in vitro. The cytotoxicity and biochemical eff ects induced by Korbazol were investigated in the murine cell lines 4T1, B16 and BCL1. Methods: The cytotoxic activity of the tested compound was assessed using a colorimetric MTT assay. The concentration of the superoxide anion radical (O 2.-) and the activity of superoxide dismutase (SOD) in the samples were determined using spectrophotometric methods. The MDA content was determined using a TBA assay. Results: We found that Korbazol induced cell toxicity, an increased the concentration of the lipid peroxidation end product MDA, decreased superoxide dismutase activity and increased superoxide anion formation. Conclusions: Altogether, these results suggest that oxidative stress is involved in Korbazol-induced cytotoxicity in the investigated cell lines

Chronic Hepatitis C: Conspectus of immunological events in the course of fibrosis evolution.

In chronically infected HCV patients emergence and evolution of fibrosis, as a consequence of virus persistence, can be considered as an indicator of disease advancement. Therefore the aim of this study was to correlate alterations of immune response in chronic HCV patients with liver histopathology. Sera cytokine levels and frequency of circulating and liver infiltrating cells were evaluated using 13plex Kit Flow Cytomix, flow cytometry and immunohistochemistry. We found that the number of circulating T lymphocytes (including CD4+, CD8+ and Treg) and B lymphocytes, as well as DCs, was higher in patients with no fibrosis than in healthy subjects. In patients with fibrosis frequency of these cells decreased, and contrarily, in the liver, number of T and B lymphocytes gradually increased with fibrosis. Importantly, in patients with advanced fibrosis, liver infiltrating regulatory T cells and DC-SIGN+ mononuclear cells with immunosuppressive and wound-healing effector functions were abundantly present. Cytokine profiling showed predominance of proinflammatory cytokines in patients with no fibrosis and a tendency of decline in level of all cytokines with severity of liver injury. Lower but sustained IL-4 production refers to Th2 predominance in higher stages of fibrosis. Altogether, our results reveal graduall alterations of immunological parameters during fibrosis evolution and illustrate the course of immunological events through disease progression

Correction: Chronic Hepatitis C: Conspectus of immunological events in the course of fibrosis evolution.

[This corrects the article DOI: 10.1371/journal.pone.0219508.]

Chronic hepatitis C: Conspectus of immunological events in the course of fibrosis evolution

© 2019 Baskic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. In chronically infected HCV patients emergence and evolution of fibrosis, as a consequence of virus persistence, can be considered as an indicator of disease advancement. Therefore the aim of this study was to correlate alterations of immune response in chronic HCV patients with liver histopathology. Sera cytokine levels and frequency of circulating and liver infiltrating cells were evaluated using 13plex Kit Flow Cytomix, flow cytometry and immuno-histochemistry. We found that the number of circulating T lymphocytes (including CD4+, CD8+ and Treg) and B lymphocytes, as well as DCs, was higher in patients with no fibrosis than in healthy subjects. In patients with fibrosis frequency of these cells decreased, and contrarily, in the liver, number of T and B lymphocytes gradually increased with fibrosis. Importantly, in patients with advanced fibrosis, liver infiltrating regulatory T cells and DC-SIGN+ mononuclear cells with immunosuppressive and wound-healing effector functions were abundantly present. Cytokine profiling showed predominance of proinflammatory cytokines in patients with no fibrosis and a tendency of decline in level of all cytokines with severity of liver injury. Lower but sustained IL-4 production refers to Th2 predominance in higher stages of fibrosis. Altogether, our results reveal graduall alterations of immunological parameters during fibrosis evolution and illustrate the course of immunological events through disease progression