SPECTROPHOTOMETRIC DETERMINATION OF DASATINIB IN PHARMACEUTICAL FORMULATIONS

Objective: A new, simple, sensitive, precise and reproducible bioanalytical method was developed for the determination of Dasatinib in pharmaceutical formulations with Chloranilic acid. Methods: The method is based on formation of violet colored complex. The UV spectrum of Dasatinib in methanol showed λ max at 521 nm. Beer’s law is valid in the concentration range of 10-60 μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-60 μg/ml with regression equation y = 0.021x-0.083 and regression correlation coefficient r2= 0.997. Moreover, the method was found to be highly sensitive with LOD (2.96μg/ml) and LOQ (8.98μg/ml). Conclusion: Based on results the proposed method can be successfully applied for the assay of Dasatinib in various pharmaceutical dosageforms

VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF IMATINIB IN BULK AND FORMULATION

Objective: A new, simple, sensitive, precise and reproducible UV visible spectrophotometric method was developed for the determination of Imatinib in pharmaceutical formulations with alizarin. Methods: The method is based on formation of yellow-colored complex. The UV spectrum of Imatinib in methanol showed λ max at 431 nm. Beer’s law is valid in the concentration range of 10-70 μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-70 μg/ml with regression equation y =0.013x-0.017 and regression correlation coefficient r2= 0.997. Moreover, the method was found to be highly sensitive with LOD (4.3μg/ml) and LOQ (13.07μg/ml). Conclusion: Based on results the proposed method can be successfully applied for the assay of Imatinib in various pharmaceutical dosage forms

Pulse radiolytic studies on cis-dichlorobis-(2,2'-bipyridine)cobalt(III) and cis-dichlorobis-(1,10-phenanthroline)cobalt(III) complexes

690-694The reactions of hydrated electron (e⁻q) with Co(III) polypyridyl complexes of the type [Co(NN)2Cl2]Cl where NN = 2,2'-bipyridine (bpy), and 1,10-phenanthroline (phen) have been studied by pulse radiolysis. The rate constants for the reactions at 300 K have been evaluated to be (7.6±0.2)x10¹⁰, and (6.9±0.2)x10¹⁰ dm³ mol⁻¹ s⁻¹, respectively. Time resolved transient absorption spectra show two broad peaks at 360 and 610 nm for the bpy complex and a single broad peak at 420 nm for the phen complex at 1 μs. Comparison with reported transient spectra of the anion radicals of ligands indicates that the electron is located on the complex as a whole. The anion radicals of both the complexes initially produced, decay in the time scale of ~80 μs. Steady state absorption spectra on irradiation point out to breakdown of the phen complex, and the bpy-Co(III) complex is reduced to Co(II) complex. Conductance of the solution substantially increases on irradiation for both the complexes and can be attributed to aquation/de-ligation of the phen complex. The phen complex anion radical undergoes aquation/de-ligation by intramolecular electron transfer leading to dissociation of the complex. For bpy complex the conductance increases due to the release of chloride ions and reduction to Co(II) complex species is observed

Metalloenzyme biosensors:Part 1 - Amperometric tyrosinase biosensor for phenols using composite biopolymer matrix

7-10An amperometric biosensor has been prepared by immobilizing plant tyrosinase enzyme in a novel composite polysaccharide gel matrix on the tip of a glassy carbon electrode and used for the measurements of Dopa substrates in aqueous medium in the concentration range 10-4 to 10-2 mol I-1. The sensor has a shelf-life of four months. Parameters affecting the performance of the sensor have also been evaluated

Synthesis, characterization and nuclease activity of copper(ll), nickel(II) and iron(II) complexes of 2-acetylpyridine oxime

533-535A few complexes of Cu(II), Ni(II) and Fe(II) with 2-acetylpyridine oxime have been synthesized and characterized by elemental analysis, IR, UV-vis spectroscopy, magnetic moments and cyclic voltammetry and their interactions with plasmid DNA are investigated

ProHSO4: An efficient catalyst for solvent-free synthesis of bis(indolyl)methanes and their in silico screening for potential biological activity

A simple, mild, and efficient protocol has been developed for the synthesis of 3,3′-bis(indolyl)methanes (BIMs) using prolinium hydrogen sulfate (ProHSO4) as a catalyst under solvent-free and support-free conditions. This mechanochemical method undergoes electrophilic substitution reaction of indole with diverse aldehydes to afford the desired BIMs in high yields at room temperature. Due to diverse pharmacological utility, the synthesized BIMs were subjected to in silico studies. The structure activity analysis of BIMs revealed their ability to bind Eg5 kinesin, Human p38 α MAP Kinase, and Human 3 alpha HSD type 3 as a potential protein target for anticancer activity. The compounds exhibited higher binding affinities with Eg5 kinesin having hydrophobic interactions with α2, α3 helices and loop5 amino acid residues. Moreover, molecular docking with Human p38 α MAP Kinase protein depicted compounds as ATP-competitive molecules interacting with hydrophobic region I, II and allosteric DFG motif. A total of 13 derivatives were synthesized and subjected for in silico screening for predicting potential biological activity. Among them, 3b, 3d, 3f, 3 g, 3i, 3j, and 3 k compounds were identified as potential inhibitors of Eg5 kinesin, Human p38 α MAP Kinase, and Human 3 alpha HSD type 3, and their evaluation in the biological experimental system is warranted

<span style="font-size:12.0pt;line-height:115%; font-family:"Times New Roman","serif";mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin;mso-ansi-language:EN-IN;mso-fareast-language: EN-US;mso-bidi-language:AR-SA">Crystal structure of cationic complex of 2[Co^III (APOTSC)₂]⁺[Co^IICI₄]^2-</span>

977-980<span style="font-size:12.0pt;line-height:115%; font-family:" times="" new="" roman","serif";mso-fareast-font-family:calibri;="" mso-fareast-theme-font:minor-latin;mso-ansi-language:en-in;mso-fareast-language:="" en-us;mso-bidi-language:ar-sa"="">The reaction between 4N-dimethyl-2-acetylpyridine-N-oxide thiosemicarbazone (HAPOTSC) ligand and cobalt chloride leads to the formation of a cationic complex containing two thiolato N-oxide thiosemicarbazone ligands, which has been characterized by spectroscopic and X-ray crystallographic studies.</span

Case Report Congenital Thrombotic Thrombocytopenic Purpura: An Unusual Case of Microangiopathic Haemolytic Anaemia in a Newborn

ABSTRACT We present an extremely rare case of hyperbilirubinemia with rapid progression leading to bilirubin encephalopathy in term neonate. Despite early recognition and intervention, death occurred as a total serum bilirubin reached 25 mg/dl. It was a case of Coomb&apos;s negative microangiopathic haemolytic anaemia in a newborn period which is autosomal recessive inheritance i.e. Upshaw-Schulman Syndrome. (Congenital thrombotic thrombocytopenic purpura) characterised by numerous schistocytes on peripheral blood smear, thrombocytopenia , increased reticulocyte count, increased bilirubin and LDH level. This rare disease is often misdiagnosed especially in newborn baby. So we present this case not only for its variety but also for to create more awareness among pathologist and paediatrician as treatment protocol entirely differ