Histochemical Distribution of Carbonic Anhydrase in Rat and Rabbit Lacrimal Gland

Purpose. The purpose of this study was to examine the histochemical distribution of carbonic anhydrase (CA) in lacrimal glands from rats and rabbits; and to determine if age-and/or sex-related differences exist in the amount and distribution of CA in the rat lacrimal gland. Methods. Lacrimal glands from young (3-12 wk) and aged (2-2.5 yr), male and female F344 rats and male rabbits were fixed in 1% paraformaldehyde and embedded in glycolmethacrylate. CA histochemistry was performed on 2-Mm sections. The distribution of CA activity was determined by morphometric analysis