74 research outputs found
Blood-Feeding Induces Reversible Functional Changes in Flight Muscle Mitochondria of Aedes aegypti Mosquito
Background: Hematophagy poses a challenge to blood-feeding organisms since products of blood digestion can exert
cellular deleterious effects. Mitochondria perform multiple roles in cell biology acting as the site of aerobic energytransducing
pathways, and also an important source of reactive oxygen species (ROS), modulating redox metabolism.
Therefore, regulation of mitochondrial function should be relevant for hematophagous arthropods. Here, we investigated
the effects of blood-feeding on flight muscle (FM) mitochondria from the mosquito Aedes aegypti, a vector of dengue and
yellow fever.
Methodology/Principal Findings: Blood-feeding caused a reversible reduction in mitochondrial oxygen consumption, an
event that was parallel to blood digestion. These changes were most intense at 24 h after blood meal (ABM), the peak of
blood digestion, when oxygen consumption was inhibited by 68%. Cytochromes c and a+a3 levels and cytochrome c
oxidase activity of the electron transport chain were all reduced at 24 h ABM. Ultrastructural and molecular analyses of FM
revealed that mitochondria fuse upon blood meal, a condition related to reduced ROS generation. Consistently, BF induced
a reversible decrease in mitochondrial H2O2 formation during blood digestion, reaching their lowest values at 24 h ABM
where a reduction of 51% was observed.
Conclusion: Blood-feeding triggers functional and structural changes in hematophagous insect mitochondria, which may
represent an important adaptation to blood feedin
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