4 research outputs found

    МСнингококковая ΠΏΠΎΠ»ΠΈΠ²Π°ΠΊΡ†ΠΈΠ½Π° Π½Π° основС ΠΎΡ‡ΠΈΡ‰Π΅Π½Π½ΠΎΠΉ IgAI ΠΏΡ€ΠΎΡ‚Π΅Π°Π·Ρ‹

    Get PDF
    IgA1-protease allocated from the culture N. meningitidis serogroup A. As original materials were used three different intermediate products of vaccine production: cultural fluid, cetavlon supernatant and cetavlon precipitate. IgA1-protease was used to evaluate their protectivity and immunogenity. It was shown, that isolated IgA1 protease from the meningococcus serogroup A is able to protect mice, infected by meningococcus serogroup B.IgA1 ΠΏΡ€ΠΎΡ‚Π΅Π°Π·Π°, выдСлСнная ΠΌΠ΅Ρ‚ΠΎΠ΄Π°ΠΌΠΈ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ΠΈΠ²Π½ΠΎΠΉ Ρ…ΠΈΠΌΠΈΠΈ ΠΈΠ· ΠΏΡ€ΠΎΠΌΠ΅ΠΆΡƒΡ‚ΠΎΡ‡Π½Ρ‹Ρ… ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΎΠ² очистки ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ полисахаридной сСрогруппы А Π²Π°ΠΊΡ†ΠΈΠ½Ρ‹, ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ‚ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π½ΠΎΠΉ ΠΈΠΌΠΌΡƒΠ½ΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΈ ΠΏΡ€ΠΎΡ‚Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒΡŽ для Π»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€Π½Ρ‹Ρ… ΠΌΡ‹ΡˆΠ΅ΠΉ, обСспСчивая ΠΈΡ… Π·Π°Ρ‰ΠΈΡ‚Ρƒ ΠΎΡ‚ ΡΠΌΠ΅Ρ€Ρ‚Π΅Π»ΡŒΠ½ΠΎΠ³ΠΎ зараТСния Π²ΠΈΡ€ΡƒΠ»Π΅Π½Ρ‚Π½Ρ‹ΠΌΠΈ ΡˆΡ‚Π°ΠΌΠΌΠ°ΠΌΠΈ сСрогрупп А ΠΈ Π’ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ². ΠŸΡ€ΠΎΡ‚Π΅ΠΊΡ‚ΠΈΠ²Π½Ρ‹ΠΉ эффСкт ΠΈ нарастаниС Π°Π½Ρ‚ΠΈ-IgA1 Π°Π½Ρ‚ΠΈΡ‚Π΅Π» ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ ΠΏΡ€ΠΎΠ³Π½ΠΎΠ·ΠΈΡ€ΠΎΠ²Π°Ρ‚ΡŒ Π²Π°ΠΊΡ†ΠΈΠ½Π½Ρ‹Π΅ ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠΈ ΠΈΠ·ΡƒΡ‡Π°Π΅ΠΌΠΎΠ³ΠΎ ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚Π°, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹ΠΉ ΠΌΠΎΠΆΠ΅Ρ‚ Ρ€Π°ΡΡΠΌΠ°Ρ‚Ρ€ΠΈΠ²Π°Ρ‚ΡŒΡΡ, Π² ΠΏΠ΅Ρ€Π²ΡƒΡŽ ΠΎΡ‡Π΅Ρ€Π΅Π΄ΡŒ, Π² качСствС ΠΊΠ°Π½Π΄ΠΈΠ΄Π°Ρ‚Π° ΠΎΡ‚ΡΡƒΡ‚ΡΡ‚Π²ΡƒΡŽΡ‰Π΅ΠΉ Π΄ΠΎ настоящСго Π²Ρ€Π΅ΠΌΠ΅Π½ΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ сСрогруппы Π’ Π²Π°ΠΊΡ†ΠΈΠ½Ρ‹

    ΠœΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²Ρ‹Π΅ Π²Π°ΠΊΡ†ΠΈΠ½Ρ‹: ΠΎΡ‚ ΠΊΠ°ΠΏΡΡƒΠ»ΡŒΠ½Ρ‹Ρ… полисахаридов Π΄ΠΎ ΠΏΡ€ΠΎΡ‚Π΅Π°Π·

    Get PDF
    Microbial capsular polysaccharides for many years provided a highly practical public health vaccines for preventing meningococcal, pneumococcal and Haemophilus influenza infection, and typhoid fever. Their application in the form of conjugates with protein carriers eliminate the gap in protection against these infections in children under one year. Extremely promising turned out offered us a new generation of vaccines, which have synthetic peptides conjugated to a meningococcal polysaccharide. Thus, new approaches to the solution of the problem of meningococcal disease vaccination serogroup B were open. In recent years, Russian researchers first suggested to use IgA1 protease (one of the major virulence factors of microbes and almost identical for mentioned below infections) for prevention of such diseases as meningococcal of all serogroups, pneumococcus and hemophilia infections. Patented processes for producing of the vaccine define domestic priority of its production and use.ΠœΠΈΠΊΡ€ΠΎΠ±Π½Ρ‹Π΅ ΠΊΠ°ΠΏΡΡƒΠ»ΡŒΠ½Ρ‹Π΅ полисахариды Π½Π° протяТСнии ΠΌΠ½ΠΎΠ³ΠΈΡ… Π»Π΅Ρ‚ обСспСчивали практичСскоС Π·Π΄Ρ€Π°Π²ΠΎΠΎΡ…Ρ€Π°Π½Π΅Π½ΠΈΠ΅ высокоэффСктивными Π²Π°ΠΊΡ†ΠΈΠ½Π°ΠΌΠΈ для ΠΏΡ€ΠΎΡ„ΠΈΠ»Π°ΠΊΡ‚ΠΈΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²Ρ‹Ρ…, ΠΏΠ½Π΅Π²ΠΌΠΎΠΊΠΎΠΊΠΊΠΎΠ²Ρ‹Ρ…, Π³Π΅ΠΌΠΎΡ„ΠΈΠ»ΡŒΠ½Ρ‹Ρ… ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΉ ΠΈ Π±Ρ€ΡŽΡˆΠ½ΠΎΠ³ΠΎ Ρ‚ΠΈΡ„Π°. А ΠΈΡ… ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π² Π²ΠΈΠ΄Π΅ ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ΠΎΠ² с Π±Π΅Π»ΠΊΠΎΠ²Ρ‹ΠΌΠΈ носитСлями Π»ΠΈΠΊΠ²ΠΈΠ΄ΠΈΡ€ΠΎΠ²Π°Π»ΠΎ ΠΏΡ€ΠΎΠ±Π΅Π» ΠΏΡ€ΠΈ Π·Π°Ρ‰ΠΈΡ‚Π΅ ΠΎΡ‚ этих ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΉ Π΄Π΅Ρ‚Π΅ΠΉ Π² возрастС Π΄ΠΎ ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π³ΠΎΠ΄Π°. НСобычайно пСрспСктивным оказалось ΠΏΡ€Π΅Π΄Π»ΠΎΠΆΠ΅Π½Π½ΠΎΠ΅ Π½Π°ΠΌΠΈ Π½ΠΎΠ²ΠΎΠ΅ ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΠ΅ Π²Π°ΠΊΡ†ΠΈΠ½, Π³Π΄Π΅ ΡƒΠΆΠ΅ синтСтичСскиС ΠΏΠ΅ΠΏΡ‚ΠΈΠ΄Ρ‹ ΠΊΠΎΠ½ΡŠΡŽΠ³ΠΈΡ€ΠΎΠ²Π°Π»ΠΈΡΡŒ с полисахаридами ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ². Π’Π°ΠΊΠΈΠΌ ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ, Π±Ρ‹Π»ΠΈ ΠΎΡ‚ΠΊΡ€Ρ‹Ρ‚Ρ‹ ΠΏΠΎΠ΄Ρ…ΠΎΠ΄Ρ‹ ΠΊ Ρ€Π΅ΡˆΠ΅Π½ΠΈΡŽ ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΡ‹ Π²Π°ΠΊΡ†ΠΈΠ½ΠΎΠΏΡ€ΠΎΡ„ΠΈΠ»Π°ΠΊΡ‚ΠΈΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΈ сСрогруппы Π’. Π’ послСдниС Π³ΠΎΠ΄Ρ‹ отСчСствСнныС исслСдоватСли Π²ΠΏΠ΅Ρ€Π²Ρ‹Π΅ ΠΏΡ€Π΅Π΄Π»ΠΎΠΆΠΈΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Ρ‚ΡŒ для ΠΏΡ€ΠΎΡ„ΠΈΠ»Π°ΠΊΡ‚ΠΈΠΊΠΈ ΠΌΠ΅Π½ΠΈΠ½Π³ΠΎΠΊΠΎΠΊΠΊΠΎΠ²Ρ‹Ρ… ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΉ всСх сСрогрупп, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΉ, обусловлСнных ΠΏΠ½Π΅Π²ΠΌΠΎΠΊΠΎΠΊΠΊΠ°ΠΌΠΈ ΠΈ Π³Π΅ΠΌΠΎΡ„ΠΈΠ»Π°ΠΌΠΈ, ΠΎΠ΄ΠΈΠ½ ΠΈΠ· Π³Π»Π°Π²Π½Ρ‹Ρ… Ρ„Π°ΠΊΡ‚ΠΎΡ€ΠΎΠ² вирулСнтности этих ΠΌΠΈΠΊΡ€ΠΎΠ±ΠΎΠ² β€” IgA1 ΠΏΡ€ΠΎΡ‚Π΅Π°Π·Ρƒ, практичСски ΠΈΠ΄Π΅Π½Ρ‚ΠΈΡ‡Π½ΡƒΡŽ для всСй ΡƒΠΊΠ°Π·Π°Π½Π½ΠΎΠΉ Π³Ρ€ΡƒΠΏΠΏΡ‹ ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΉ. Π—Π°ΠΏΠ°Ρ‚Π΅Π½Ρ‚ΠΎΠ²Π°Π½Π½Ρ‹Π΅ способы получСния этой Π²Π°ΠΊΡ†ΠΈΠ½Ρ‹ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡŽΡ‚ отСчСствСнный ΠΏΡ€ΠΈΠΎΡ€ΠΈΡ‚Π΅Ρ‚ Π΅Π΅ производства ΠΈ примСнСния

    Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis

    No full text
    A method of the isolation and purification of IgA1 protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and precipitate obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been developed. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 ΞΌg. It was shown that IgA1 protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B. Β© 2010 Pleiades Publishing, Ltd

    Isolation and determination of the activity of IgA1 protease from Neisseria meningitidis

    No full text
    A method of the isolation and purification of IgA1 protease from a culture of Neisseria meningitidis serogroup A has been developed. Three inactivated intermediates of the production of the meningococcal vaccine, a culture liquid, as well as a supernatant and precipitate obtained by the precipitation of bacterial cells by cetavlon, served as a starting material. The purity of IgA1 protease was determined by SDS-PAGE. An immunoenzyme assay for determining the IgA1 protease activity has been developed. The yield of the enzyme with a specific activity of 0.5 to 4 million units/mg from 103 g of the cetavlon precipitate (40 l of culture liquid) was about 600 ΞΌg. It was shown that IgA1 protease isolated from serogroup A meningococcus is capable of protecting experimental animals (mice) infected with meningococcus of serogroup B. Β© 2010 Pleiades Publishing, Ltd
    corecore