Prévention de la transition du cancer in situ vers un cancer invasif chez les femmes en surpoids/obèses : rôle des cellules myoépithéliales

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Identification de facteurs préventifs de la transition du CCIS (carcinome canalaire in situ) vers un CCI (carcinome canalaire invasif) chez les femmes en surpoids ou obèses

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Obesity and cancer: role of adipose cells in preventing the transition from in situ to invasive breast cancer in a two-cell fluorescent spheroid model

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Evolution of three dimensional skin equivalent models reconstructed in vitro by tissue engineering

Since the culture of keratinocytes on feeder layers, research to produce skin equivalents has been motivated by the challenge of treating large burns and chronic wounds and by European regulations which both require proof of the innocuousness and the effectiveness of cosmetic products, and which forbid animal testing. The dynamism in fundamental research, dermocosmetology and the pharmaceutical industry has led to the evolution and complexification of reconstructed skin. The Collagen-GAG-Chitosan sponge, as well as the self-assembly model, allow dermal reconstruction in which the neosynthesized extracellular matrix contains all of the desired macromolecules. It is deposited forming an ultrastructurally organised architecture. The quality of the dermis obtained allows the development and regeneration of a pluristratified and differentiated epidermis firmly anchored by an organised dermal-epidermal junction. Evolution of reconstructed skin into models which are more and more similar to the physiological skin results in higher graft take rates in the treatment of burns and chronic wounds, and brings to research, to dermocosmetology and to the pharmaceutical industry, a wide range of products such as pigmented, endothelialized, immunocompetent, and now adipose reconstructed skins. The present review will mainly concentrate on the latest developments in skin engineering and will mostly concern the studies carried out by our groups. Key words: burn trea

A novel conjunctive microenvironment derived from human subcutaneous adipose tissue contributes to physiology of its superficial layer

International audienceAbstract Background In human subcutaneous adipose tissue, the superficial fascia distinguishes superficial and deep microenvironments showing extensions called retinacula cutis. The superficial subcutaneous adipose tissue has been described as hyperplastic and the deep subcutaneous adipose tissue as inflammatory. However, few studies have described stromal-vascular fraction (SVF) content and adipose-derived stromal/stem cells (ASCs) behavior derived from superficial and deep subcutaneous adipose tissue. In this study, we analyzed a third conjunctive microenvironment: the retinacula cutis superficialis derived from superficial subcutaneous adipose tissue. Methods The samples of abdominal human subcutaneous adipose tissue were obtained during plastic aesthetic surgery in France (Declaration DC-2008-162) and Brazil (Protocol 145/09). Results The SVF content was characterized in situ by immunofluorescence and ex vivo by flow cytometry revealing a high content of pre-adipocytes rather in superficial subcutaneous adipose tissue microenvironment. Adipogenic assays revealed higher percentage of lipid accumulation area in ASCs from superficial subcutaneous adipose tissue compared with retinacula cutis superficialis ( p < 0.0001) and deep subcutaneous adipose tissue ( p < 0.0001). The high adipogenic potential of superficial subcutaneous adipose tissue was corroborated by an up-regulation of adipocyte fatty acid-binding protein (FABP4) compared with retinacula cutis superficialis ( p < 0.0001) and deep subcutaneous adipose tissue ( p < 0.0001) and of C/EBPα (CCAAT/enhancer-binding protein alpha) compared with retinacula cutis superficialis ( p < 0.0001) and deep subcutaneous adipose tissue ( p < 0.0001) microenvironments. Curiously, ASCs from retinacula cutis superficialis showed a higher level of adiponectin receptor gene compared with superficial subcutaneous adipose tissue ( p = 0.0409), widely known as an anti-inflammatory hormone. Non-induced ASCs from retinacula cutis superficialis showed higher secretion of human vascular endothelial growth factor (VEGF), compared with superficial ( p = 0.0485) and deep ( p = 0.0112) subcutaneous adipose tissue and with adipogenic-induced ASCs from superficial ( p = 0.0175) and deep ( p = 0.0328) subcutaneous adipose tissue. Furthermore, ASCs from retinacula cutis superficialis showed higher secretion of Chemokine (C–C motif) ligand 5 (CCL5) compared with non-induced ( p = 0.0029) and induced ( p = 0.0089) superficial subcutaneous adipose tissue. Conclusions This study highlights the contribution to ASCs from retinacula cutis superficialis in their angiogenic property previously described for the whole superficial subcutaneous adipose tissue besides supporting its adipogenic potential for superficial subcutaneous adipose tissue

Prévention de la transition du cancer mammaire in situ vers un cancer invasif chez les femmes en surpoids/obèses : développement d'un modèle de tumoroïdes bicellulaires fluorescents

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Obésité et progression tumorale : Optimisation d’un modèle 3D de tumoroïdes bicellulaires fluorescents mimant le cancer canalaire in situ dans un microenvironnement adipeux et inflammatoir

International audienc

Obésité et progression tumorale : Optimisation d’un modèle 3D de tumoroïdes bicellulaires fluorescents mimant le cancer canalaire in situ dans un microenvironnement adipeux et inflammatoir

International audienc

Prévention de la transition du cancer mammaire in situ vers un cancer invasif chez les femmes en surpoids/obèses : développement d'un modèle de tumoroïdes bicellulaires fluorescents

International audienc

Développement d’un modèle 3D de tumoroïdes bicellulaires fluorescents mimant le cancer canalaire in situ dans un microenvironnement adipeux et inflammatoire

International audienc