Anti-protozoal activity and metabolomic analyses of Cichorium intybus L. against Trypanosoma cruzi

Chagas disease, caused by the protozoa Trypanosoma cruzi, is a potentially life-threatening parasitic zoonosis infecting 6-7 million people worldwide, mainly in Latin America. Due to the limited numbers of drugs available against this neglected disease and their frequent adverse effects, novel anti-chagasic agents are urgently needed. Cichorium intybus L. (chicory) is a bioactive plant with potent activity against parasitic nematodes, but its effects on protozoans are poorly known and no studies have explored its trypanocidal potential. Here, we investigated the activity of C. intybus against extracellular and intracellular stages of T. cruzi, including the prediction of trypanocidal compounds by metabolomic analyses and bioactivity-based molecular networking. Purified C. intybus extracts were prepared from leaves and roots of five C. intybus cultivars (cv. \u27Benulite\u27, \u27Goldine\u27, \u27Larigot\u27, \u27Maestoso\u27 and \u27Spadona\u27). All C. intybus extracts induced concentration-dependent effects against T. cruzi trypomastigotes. C. intybus leaf extracts had higher trypanocidal selectivity and lower cytotoxicity on mammalian cells than root extracts. The leaf extract of C. intybus cv. Goldine also significantly reduced the number of mammalian cells infected with T. cruzi amastigotes. Metabolomic and bioactivity-based molecular networking analyses revealed 11 compounds in C. intybus leaves strongly linked with activity against trypomastigotes, including the sesquiterpene lactone lactucin, and flavonoid- and fatty acid-derivatives. Furthermore, seven distinct C. intybus molecules (including two sesquiterpene lactone-derivatives) were predicted to be involved in reducing the number of mammalian cells infected with amastigotes. This is the first report of the anti-protozoal activity of C. intybus against trypanosomatid parasites and expands our understanding of the anti-parasitic effects of this plant and its bioactive metabolites. Further studies to elucidate the anti-protozoal compound(s) in C. intybus and their mode(s) of action will improve our knowledge of using this bioactive plant as a promising source of novel broad-spectrum anti-parasitic compounds with associated health benefits and biomedical potential

Sequence conservation and structural features that are common within TRP channels

TRP proteins are a large family of cation-selective channels, surpassed in variety only by voltage-gated potassium channels. Detailed molecular mechanisms governing how membrane voltage, ligand binding, or temperature can induce conformational changes promoting the open state in TRP channels are still a matter of debate. Aiming to unveil distinctive structural features common to the transmembrane domains within the TRP family, we performed phylogenetic reconstruction, sequence statistics, and structural analysis over a large set of TRP channel genes. Here we report an exceptionally conserved set of residues. This fingerprint is composed of twelve residues localized at equivalent three-dimensional positions in TRP channels from the different subtypes. Moreover, these amino acids are arranged in three groups, connected by a set of aromatics located at the core of the transmembrane structure. We hypothesize that differences in the connectivity between these different groups of residues harbors the apparent differences in coupling strategies used by TRP subgroups

Glacier runoff projections and their multiple sources of uncertainty in the Patagonian Andes (40-56°S)

This dataset contains the catchment scale results of the study: "Assessing the glacier projection uncertainties in the Patagonian Andes (40-56°S) from a catchment perspective". The results are disaggregated in the following files (for more details, please read the README file): - basins_boundaries.zip: Contains the polygons (in .shp format) of the studied catchments. Each catchment is identified by its "basin_id". - dataset_historical.csv: Summarises the historical conditions of each glacier at the catchment scale (area, volume and reference climate). - dataset_future.csv: Summarises the future glacier climate drivers and their impacts at the catchment scale. - dataset_signatures.csv: Summarises the glacio-hydrological signatures for each catchment. The metrics are calculated for the variables "total glacier runoff (tr)" and "melt on glacier (mg)". The main source of uncertainty in each catchment was the source that accumulated most RMSE loss

Physical and geochemical data on a drill core from the semi-arid Coastal Cordillera, Chile

This dataset contains petrophysical, geochemical, and mineralogical data from a drilling core from the Coastal Cordillera, Chile. The drilling campaign in the semi-arid field site Reserve Santa Gracia was conducted in the framework of the “EarthShape” project (DFG SPP1803) to study deep weathering along a climate gradient. Previous studies in this area found that the weathering front is located much deeper than expected (Oeser et al., 2018). To explore the weathering profile and the depth of the weathering front, we performed various geochemical, petrophysical, and mineralogical analyses. The drilling campaign was conducted in March and April 2019, using the wireline drilling method with a standard industry truck-mounted PQ3-sized (85 mm core diameter, 123 mm hole diameter) rotary drilling rig (Sondajes Araos E.I.R.L.). A detailed description of the drilling activities is given in Krone et al. (2021). The retrieved core runs with a maximum length of 1.5 m were drilled using potable water, with added contamination control tracer for further microbiological analyses of the rock. As basis for our detailed study of deep weathering we determined the porosity, density, specific surface area, elemental composition, mineralogical composition, Fe oxidation, and the degree of weathering from chemical depletion, volumetric strain, and the weathering rate using the in situ cosmogenic nuclide beryllium-10 (10Be)

Soundscape Attributes Translation Project (SATP) Dataset

The data and audio included here were collected for the Soundscape Attributes Translation Project (SATP). First introduced in Aletta et. al. (2020), the SATP is an attempt to provide validated translations of soundscape attributes in languages other than English. The recordings were used for headphones - based listening experiments. The data are provided to accompany publications resulting from this project and to provide a unique dataset of 1000s of perceptual responses to a standardised set of urban soundscape recordings. This dataset is the result of efforts from hundreds of researchers, students, assistants, PIs, and participants from institutions around the world. We have made an attempt to list every contributor to this Zenodo repo; if you feel you should be included, please get in touch. Citation: If you use the SATP dataset or part of it, please cite our paper describing the data collection and this dataset itself. Overview: The SATP dataset consists of 27 30-sec binaural audio recordings made in urban public spaces in London and one 60 sec stereo calibration signal. The recordings were made at locations as reported in Table 1 of the README.md (Recording locations), at various times of day by an operator wearing a binaural kit consisting of BHS II microphones and a SQobold (HEAD acoustics) device. Recordings were then exported to WAV via the ArtemiS SUITE software, using the original dynamic range from HDF. The listening experiment and the calibration procedure were intended for a headphone playback system (Sennheiser HD650 or similar open-back headphones recommended).  The recordings were selected from an initial set of 80 recordings through a pilot study to ensure the test set had an even coverage of the soundscape circumplex space. These recordings were sent to the partner institutions (see Table 2 of the README.md) and assessed by approximately 30 participants in the institution's target language. The questionnaire used in each assessment is a translation of Method A Questionnaire, ISO 12913-2:2018. Each institution carried out their own lab experiment to collect data, then submitted their data to the team at UCL to compile into a single dataset. Some institutions included additional questions or translation options; the combined dataset (`SATP Dataset v1.x.xlsx`) includes only the base set of questions, the extended set of questions from each institution is included in the `Institution Datasets` folder. In all, SATP Dataset v1.2 contains 17,441 samples, including 645 participants, for 27 recordings, in 19 languages with contributions from 29 institutions. Format: The audio recordings are provided as 24 bit, 48 kHz, stereo WAV files. The combined dataset and Institutional datasets are provided as long tidy data tables in .xlsx files. Calibration: The recommended calibration approach was based on the open-circuit voltage (OCV) procedure which was considered most accessible but other calibration procedures are also possible (Lam et. al. (2022)). The provided calibration file is a computer generated sine wave at 1kHz, matching a sine wave recorded using the exact same setup at SPL of 94 dB. In case of the calibration signal playback level set to match SPL of 94 dB at the eardrum, all the 27 samples should be reproduced at realistic loudness. More details on OCV calibration procedure and other options you can find in Lam et. al. (2022) and the attached documentation. PLEASE DO NOT EXPOSE YOURSELF NOR THE PARTICIPANTS TO THE CALIBRATION SIGNAL SET AT THE REALISTIC LEVEL AS IT CAN CAUSE HARM. License and reuse: All SATP recordings are provided under the Creative Commons Attribution 4.0 International (CC BY 4.0) License and are free to use. We encourage other researchers to replicate the SATP protocol and contribute new languages to the dataset. We also encourage the use of these recordings and the perceptual data for further soundscape research purposes. Please provide the proper attribution and get in touch with the authors if you would like to contribute a new translation or for any other collaborations