Additional file 1: Figure S1. of Improvements to the HITS-CLIP protocol eliminate widespread mispriming artifacts

The sequences of the two most common adaptor/primer pairs do not influence the frequency of mispriming. Percentage of peaks containing the first six bases of the 3′ adaptor sequence (allowing for one mismatch) between positions −25 and +75 in each peak (highlighted in grey in Fig. 1a), minus the expected frequency (calculated using 1 x 106 randomly sampled exonic sequences of 200 bp). Adaptors/primers from [1] blue, n = 17) and the Illumina Small RNA Kit (vermillion, n = 17) are included. (PDF 19 kb