Transmission of primary resistance mutation K103N in a cluster of Belgian young patients from different risk groups

Background: We analysed the distribution of an HIV-1 subtype B strain resistant to efavirenz and nevirapine among incident infections in the Belgian population. Method: The Belgian AIDS reference laboratories searched their databases for HIV-1 subtype B sequences harbouring the K103N mutation in the reverse transcriptase (RT) or the C67S and V77I mutations in the protease (PR). We included the earliest RT sequence available of drug-naïve patients as well as sequences related to treatment failure. Fifty sequences were aligned omitting the codon 103 and submitted to phylogenetic analysis. Epidemiological data were collected through the Institute of Public Health national database. In addition, three sequences from the cluster were analysed by deep sequencing using the Roche GS Junior platform. Results: Phylogenetic analysis revealed the presence of a 24 virus sequences cluster. All except one of those sequences resulted from patients who were ARV-naïve at the time of sampling, and 21 had the K103N mutation. Two thirds of the clustered patients were infected through homosexual or bisexual contacts while the others were heterosexuals. No case was related to migrants contaminated abroad. Fifteen of the clustered patients were diagnosed between January 2011 and June 2012; 87% of them were aged between 20 and 29 at the time of diagnosis. Interestingly, 60% of them reside in the province of Namur. Deep sequencing analysis of 3 individuals sampled near seroconversion revealed no other resistance mutations at a frequency > 1% than those already picked up by Sanger sequencing (RT A98S, K103N; PR V77I), except the RT V90I. Conclusion: We identified a transmission cluster of drug resistant HIV-1 variants mainly including homo- and heterosexual young adults. Most individuals are of Belgian origin and are living around the city of Namur (Belgium). The K103N mutation had no apparent impact on transmission fitness as its spread raised during the last years. These observations may impact on local prevention and ARV prophylaxis strategies

Infected abdominal aortic aneurysm treated by in situ replacement with cryopreserved arterial homograft.

peer reviewedInfected abdominal aortic aneurysms are uncommon but not rare (1-3% of all abdominal aortic aneurysms). This life-threatening disease can lead to rapid uncontrolled sepsis and/or aortic rupture. We report one case that underlines two notions. Firstly computed tomography is effective to detect early stages of the pathology providing complete depiction of the anatomical abnormalities. Secondly infected aortic aneurysm can be successfully treated by antibiotherapy and in situ replacement with cryopreserved arterial homograft

La co-infection par le virus de l'immunodéficience humaine (VIH) et le virus de l'hépatite C (VHC) ou le virus de l'hépatite B (VHB)

Co-infection HIV/HCV or HBV is frequent because of the common ways of transmission of these various viruses. HIV-infected patients are living longer since the introduction of highly active antiretroviral therapy. However, co-infection with the hepatitis C virus (HCV) or hepatitis B virus leads to increased morbidity from liver disease and higher overall mortality. Noxious interactions between these viruses are currently well-known in particular acceleration by the HIV of the natural history of HCV or HBV infection. Recent progress was made in the diagnosis and management of the patients Co-infected by HIV/HCV or HBV Although remarkable, this progress does not occult the need for continuing the preventive measures to reduce the acquisition of new infections and the development of new drugs more effective and better-tolerated to ensure a more important therapeutic success for all the Co-infected patients