ACTIVIDAD ANTI-INFLAMATORIA in vivo Y TOXICIDAD AGUDA DE EXTRACTOS METANOLICOS DE HOJAS DE PLANTAS SILVESTRES Y CULTIVOS DE SUSPENSIONES CELULARES DE Buddleja cordata Kunth (Buddlejaceae)

Buddleja cordata is a species used by Mexican folk medicine for treating illnesses related to inflammation such as skin wounds and arthritis. It bio-synthesizes metabolites such as verbascoside that contributes to its ethno-therapeutic properties as anti-inflammatory remedy. HPLC analysis showed that the methanolic extract from cell suspension cultures (Bc-Cc) and wild plant leaves (Bc-Wp) contained verbascoside, but concentration was higher in Bc-Cc (87.48 mg/g) than in wild plant (47.34 mg/g). In the acute toxicity model, none of the extracts generated any lethality or adverse eects. In acute inflammation model induced with TPA, Bc-Cc extract showed a greater edema inhibition at 2 mg/ear (61.72%), as well for carrageenan model at 200 mg/kg(48.87%). Bc-Wp showed lesser anti-inflammatory efect in both acute inflammation models than Bc-Cc. For Adjuvant-induced arthritis both extracts at 250 mg/kg generated a moderate inhibition over edema ( 33%) at day 28, and they were statistically no different to phenilbutazone. The culture in suspension of B. cordata obtained by biotechnological process contains greater amount of verbascosido and showed better anti-inflammatory activity; thus, representing a source for obtaining this type of secondary metabolite of pharmacological interes

Photoprotective Activity of Buddleja cordata Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts

The research on compounds exhibiting photoprotection against ultraviolet radiation (UVR) is a matter of increasing interest. The methanolic extract of a cell culture of Buddleja cordata has potential photoprotective effects as these cells produce phenolic secondary metabolites (SMs). These metabolites are attributed with biological activities capable of counteracting the harmful effects caused by UVR on skin. In the present work, the methanolic extract (310–2500 µg/mL) of B. cordata cell culture showed a photoprotective effect on UVB-irradiated 3T3-Swiss albino fibroblasts with a significant increase in cell viability. The greatest photoprotective effect (75%) of the extract was observed at 2500 µg/mL, which was statistically comparable with that of 250 µg/mL verbascoside, used as positive control. In addition, concentrations of the extract higher than 2500 µg/mL resulted in decreased cell viability (≤83%) after 24 h of exposure. Phytochemical analysis of the extract allowed us to determine that it was characterized by high concentrations of total phenol and total phenolic acid contents (138 ± 4.7 mg gallic acid equivalents and 44.01 ± 1.33 mg verbascoside equivalents per gram of extract, respectively) as well as absorption of UV light (first and second bands peaking at 294 and 330 nm, respectively). Some phenylethanoid glycosides were identified from the extract

Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts

The research on compounds exhibiting photoprotection against ultraviolet radiation (UVR) is a matter of increasing interest. The methanolic extract of a cell culture of Buddleja cordata has potential photoprotective effects as these cells produce phenolic secondary metabolites (SMs). These metabolites are attributed with biological activities capable of counteracting the harmful effects caused by UVR on skin. In the present work, the methanolic extract (310–2500 µg/mL) of B. cordata cell culture showed a photoprotective effect on UVB-irradiated 3T3-Swiss albino fibroblasts with a significant increase in cell viability. The greatest photoprotective effect (75%) of the extract was observed at 2500 µg/mL, which was statistically comparable with that of 250 µg/mL verbascoside, used as positive control. In addition, concentrations of the extract higher than 2500 µg/mL resulted in decreased cell viability (≤83%) after 24 h of exposure. Phytochemical analysis of the extract allowed us to determine that it was characterized by high concentrations of total phenol and total phenolic acid contents (138 ± 4.7 mg gallic acid equivalents and 44.01 ± 1.33 mg verbascoside equivalents per gram of extract, respectively) as well as absorption of UV light (first and second bands peaking at 294 and 330 nm, respectively). Some phenylethanoid glycosides were identified from the extract

<i>Arnica montana</i> Cell Culture Establishment, and Assessment of Its Cytotoxic, Antibacterial, <i>α</i>-Amylase Inhibitor, and Antioxidant In Vitro Bioactivities

Arnica montana cell suspension culture could be a sustainable source of a vegetal material producer of secondary metabolites (SMs) possessing biological effects. Different plant growth regulator concentrations (0–5 mg/L) were tested in foliar explants to induce a callus that was used to establish a cell suspension culture. Growth kinetics was carried out for 30 days. A methanolic extract obtained from biomass harvested at 30 days of growth kinetics was fractionated, and three fractions were tested for bioactivities. We induced a callus with 1 mg/L of picloram and 0.5 mg/L of kinetin in foliar explants, which allowed for the establishment of a cell suspension culture, and the latter had the highest total SMs contents at day 30. Three fractions showed differences in total SMs contents, with the highest values per gram as follows: 270 mg gallic acid equivalent for total phenolic content, 200 mg quercetin equivalent for total flavonoid content, 83 mg verbascoside equivalent for total phenolic acid content, and 396 mg parthenolide equivalent for total sesquiterpene lactone content. The best bioactivities were 2–6 µg/mL for the 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical, 30% cellular viability of lymphoma cells at 40 µg/mL, 17% inhibition against Escherichia coli and Staphylococcus aureus at 8 µg/disk, and α-amylase inhibition at 12% with 10 µg/mL. The total SMs contents were correlated with bioactivities