10 research outputs found
Sociodemographic characteristics of the study sample (n = 925) and of respondents excluded from the analysis (n = 557), COSEMIL survey.
<p>Sociodemographic characteristics of the study sample (n = 925) and of respondents excluded from the analysis (n = 557), COSEMIL survey.</p
Factors associated with biologically confirmed STIs (diagnosed STIs)- results of univariate logistic regression among French servicewomen (n = 141) and servicemen (n = 784), COSEMIL survey.
<p>Factors associated with biologically confirmed STIs (diagnosed STIs)- results of univariate logistic regression among French servicewomen (n = 141) and servicemen (n = 784), COSEMIL survey.</p
Prevalence of STI diagnosis and STI reporting (12 past months) according to gender and age class, COSEMIL survey.
<p>Prevalence of STI diagnosis and STI reporting (12 past months) according to gender and age class, COSEMIL survey.</p
Prevalence of STIs among French service members, according to gender, and according to the origin of information (reported <i>versus</i> biologically confirmed (diagnostic STIs)), COSEMIL survey.
<p>Prevalence of STIs among French service members, according to gender, and according to the origin of information (reported <i>versus</i> biologically confirmed (diagnostic STIs)), COSEMIL survey.</p
Factors associated with biologically confirmed STIs (diagnosed STIs)- results of multivariate logistic regression (n = 753), COSEMIL survey.
<p>Factors associated with biologically confirmed STIs (diagnosed STIs)- results of multivariate logistic regression (n = 753), COSEMIL survey.</p
Flow chart for the analyses on lower STIs, COSEMIL survey.
<p>Flow chart for the analyses on lower STIs, COSEMIL survey.</p
Polymorphism of the AYE CRISPR locus in <i>A. baumannii</i>.
<p>A) Schematic representation of the CRISPR locus in three sequenced genomes and at the growing end of isolates A28, P054 and P065. Arrows show the position of primers used to amplify a portion of the locus. Specific spacers are shown with grey boxes. B) Sequence of the growing end of strain AB307 and isolate A28. The sequence flanking the last DR is in italics.</p
Genetic diversity of 56 <i>A. baumannii</i> isolates.
<p>The legend is that of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044597#pone-0044597-g001" target="_blank">Figure 1</a>.</p
Diversity of <em>Acinetobacter baumannii</em> in Four French Military Hospitals, as Assessed by Multiple Locus Variable Number of Tandem Repeats Analysis
<div><h3>Background</h3><p>Infections by <em>A. calcoaceticus</em>-<em>A. baumannii</em> (ACB) complex isolates represent a serious threat for wounded and burn patients. Three international multidrug-resistant (MDR) clones (EU clone I-III) are responsible for a large proportion of nosocomial infections with <em>A. baumannii</em> but other emerging strains with high epidemic potential also occur.</p> <h3>Methodology/Principal Findings</h3><p>We automatized a Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) protocol and used it to investigate the genetic diversity of 136 ACB isolates from four military hospitals and one childrens hospital. <em>Acinetobacter</em> sp other than <em>baumannii</em> isolates represented 22.6% (31/137) with a majority being <em>A. pittii</em>. The genotyping protocol designed for <em>A.baumannii</em> was also efficient to cluster <em>A. pittii</em> isolates. Fifty-five percent of <em>A. baumannii</em> isolates belonged to the two international clones I and II, and we identified new clones which members were found in the different hospitals. Analysis of two CRISPR-cas systems helped define two clonal complexes and provided phylogenetic information to help trace back their emergence.</p> <h3>Conclusions/Significance</h3><p>The increasing occurrence of <em>A. baumannii</em> infections in the hospital calls for measures to rapidly characterize the isolates and identify emerging clones. The automatized MLVA protocol can be the instrument for such surveys. In addition, the investigation of CRISPR/cas systems may give important keys to understand the evolution of some highly successful clonal complexes.</p> </div
Oligonucleotides used for the multiplex PCR reactions.
a<p>The forward primer is labeled with one of three dyes D2, D3 and D4. The allele range for each reaction is indicated showing that amplicons with the same dye cannot overlap.</p>b<p>Values referred to the genome of strain ATCC 17978 and considered for allele assignment.</p>c<p>The allele range for each reaction is indicated showing that amplicons with the same dye cannot overlap. Values obtained with <i>A. baumannii</i> isolates.</p