Three bacterial genomes run on R6 Oxford Nanopore MinIon Chemistry

<p>Three bacterial genomes pooled at equal DNA concentrations and run on R6 Oxford Nanopore Chemistry. Borellia burdorferi B31, Escherichia coli K12 and Streptomyces avermitilis MA 4680.</p> <p> </p> <p>Note that these have been run together as a pool with no barcoding.</p> <p> </p> <p>The Oxford Nanopore reads are contained in the file ninthtranche_renamed.fastq. The remaining FASTA files are the corresponding reference genomes included for convenience. The Lambda Control DNA spike-in sequence is also included. </p> <p> </p> <p>Bioanalyser traces and materials and methods may be found in the attached file. </p> <p> </p> <p>Version 1.91 Meterichor basecaller using 2D option.</p> <p>S.avermitilis 1.8x coverage<br>B.burgdorferi 18.1x coverage<br>Lambda control spike-in 1,132x coverage<br>E.coli 4,64kb 6702 14.2% 0.25x coverage</p> <p> </p> <p>Statistics for read lengths:</p> <p>Min read length: 5<br>Max read length: 98,366<br>Mean read length: 1998.91<br>Standard deviation of read length: 2942.24<br>Median read length: 1,040<br><br></p> <p>Statistics for numbers of reads:<br>Number of reads: 47,093</p> <p>Non-aligned reads 18051 38%<br>Aligned reads 29042 62%</p> <p>Number of reads >=1kb: 24,392</p> <p> </p> <p>Statistics for bases in the reads:</p> <p>Number of bases in all reads: 94,134,729<br>Number of bases in reads >=1kb: 82,118,285<br>GC Content of reads: 47.21 %</p> <p> </p> <p>Organism Genome size Number of reads aligning Percentage of aligned reads Mean depth of coverage</p> <p></p

The second Oxford Nanopore read ever published

<p>To our knowledge this is the second Oxford Nanopore read published. This read defines some completely uninteresting features including a 30S ribosomal subunit protein S1 and an integration host factor.</p

Additional file 1: Figure S1. of The molecular characterisation of Escherichia coli K1 isolated from neonatal nasogastric feeding tubes

Genomic analysis of E. coli sequence types using the Comprehensive Antibiotic Database (CARD: http://arpcard.mcmaster.ca ). (DOC 1740 kb