A UPLC-MS/MS METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF POMALIDOMIDE FROM HUMAN PLASMA

Objective: The present work aimed to develop a simple, rapid, specific and precise liquid chromatography-tandem mass spectrophotometric (LC–MS/MS) validated method for quantification of pomalidomide and internal standard (ISTD) Fluconazole in human plasma.Methods: 50 µl of 0.1% formic acid was added to plasma samples prior to liquid-liquid extraction (LLE) using 2.5 ml of ethyl acetate. Chromatographic separation was achieved on Xterra, RP18, 5 µ (50 x 4.6 mm) column using a mixture of 0.1% (v/v) formic acid in water to methanol at a ratio of 12:88, v/v as the mobile phase. The flow rate was 0.50 ml/min. The LC eluent was split, and approximately 0.1 ml/min was introduced into Tandem mass spectrometer using turbo Ion Spray interface at 325 °C. Quantitation was performed by transitions of m/z 260.1 precursor ion to the m/z 148.8 for pomalidomide and m/z 307.1/238.0 for fluconazole.Results: The concentrations of nine working standards showed linearity between 9.998 to 1009.650 ng/ml (r2 ≥ 0.9968). Chromatographic separation was achieved within 2 min. The average extraction recoveries of three quality control concentrations were 53.86% for pomalidomide and were within the acceptance limits. The coefficient of variation was ≤15% for intra-and inter-batch assays. The %CV of ruggedness ranges 1.32 to 4.03. The % stability of short term and long term stock solution stability studies was found to be 99.01% and 98.49% respectively.Conclusion: The results obtained for specificity, linearity, accuracy, precision, ruggedness and stability studies were within limits. Thus the validated economical method was applied for pharmacokinetic studies of pomalidomide

A VALIDATED LC-MS/MS METHOD FOR PHARMACOKINETIC STUDY OF BRIVARACETAM IN HEALTHY RABBITS

Objective: A liquid chromatography-tandem mass spectrophotometric (LC–MS/MS) method was developed for quantification of brivaracetam in rabit plasma employing liquid-liquid extraction with ethyl acetate.Methods: Developed method was validated for specificity, precision, accuracy, recovery, and stability characteristics. Chromatographic separation was achieved on Chromolith C18column (100 mmx4.6 mmx5 µm) with 0.1% formic acid, adjusted to pH 3.2 as an isocratic mobile phase with a flow rate of 1.0 ml/min. the developed method was applied to assess pharmacokinetics parameters like Cmax, Tmax, t1/2 and AUC of brivaracetam in healthy rabbits.Results: The developed method was linear over the range of 0.16 to 8µg/ml. The regression equation for the analysis was Y=0.0053x+0.0018 with coefficient of correction (r2) = 0.998. The % mean recovery for brivaracetam was found to be between 95.7% to 106.5%. The mean intraday and inter-day precision of the method was found to be 0.77 to 3.72% for quality control standards. Brivaracetam showed Tmax of 1.025±0.061 and mean Cmax, AUC0®t andAUC0®a for Test formulation is 92.7±4.4, 496.21±26.4 and 504.20±30.68 respectively.Conclusion: A highly specific, rugged and rapid method with sufficiently low LLOQ was developed for analysis of routine samples of a single dose or multiple dose pharmacokinetic studies with any marketing formulation of brivaracetam

Renewable energy based electricity generation in the pacific island countries

The Pacific Island Countries (PICs) face many challenges as their small population dispersed over millions of kilometers, advances toward sustainable development. Electricity generation using renewable resources and increased access are crucial for economic and social progress. This article provides a report on the electricity generation status of various PICs and describes the steps being undertaken to ensure all the Pacific islanders have access to sustainable energy

Deformation and crystallization of Zr-based amorphous alloys in homogeneous flow regime

The purpose of this study is to experimentally investigate the interaction of inelastic deformation and microstructural changes of two Zr-based bulk metallic glasses (BMGs): Zr_(41.25)Ti_(13.75)Cu_(12.5)Ni_(10)Be_(22.5) (commercially designated as Vitreloy 1 or Vit1) and Zr_(46.75)Ti_(8.25)Cu_(7.5)Ni_(10)Be_(27.5) (Vitreloy 4, Vit4). High-temperature uniaxial compression tests were performed on the two Zr alloys at various strain rates, followed by structural characterization using differential scanning calorimetry (DSC) and transmission electron microscopy (TEM). Two distinct modes of mechanically induced atomic disordering in the two alloys were observed, with Vit1 featuring clear phase separation and crystallization after deformation as observed with TEM, while Vit4 showing only structural relaxation with no crystallization. The influence of the structural changes on the mechanical behaviors of the two materials was further investigated by jump-in-strain-rate tests, and flow softening was observed in Vit4. A free volume theory was applied to explain the deformation behaviors, and the activation volumes were calculated for both alloys

Effect of Vision Training on Serving Skill of Volley Ball Players

For the purpose of this study the investigator randomly selected thirty two volleyball players (N=32) from School of Physical Education and Sports Science, Kannur University and B S E Volley ball club Aleppey who were equally divided into experimental group and control group (n=16). Their age ranged from 20 to 25years. The experimental group got sports vision training according to training programme and each training session was for one hour and the total duration of the training programme was for six week. During this period, the control group was let off without any training. The effect of vision training on the serving ability was tested. The pre-test was conducted on the experimental and control group. After six weeks of training post-test was conducted. „T?test was used to analyse the data. From the statistical analysis it is evident that in serving test item significant changes were noticed after training

Peer to Peer Communication between Android Device and PC and Video Surveillance using Android Device

Peer to peer technology is used for sharing of various contents that are already saved on PC. In this mobile is one peer and PC is another peer. Connection is established between computer and android smart phone which is peer to peer networking. This system is useful for like viewing or browsing a file which is stored on a remote computer, saving image files and text files to the computer and handling control of a remote computer using a android smart phone. Not only remote computer but also we can have control over various extended devices which are connected to that computer. It also represents architecture to improve video surveillance using android Smart-phone. This system allows application dynamic composition which is increases the overall flexibility of the system. This video surveillance system satisfies all needs of dynamism which results into easy allowing use of system to different users. This system provides better surveillance by selecting access live for past videos from different locations. DOI: 10.17762/ijritcc2321-8169.15013