ALTERNATE USE OF DIFFERENT RICA PROBIOTIC BACTERIA ON SURVIVAL RATE AND PRODUCTION OF CULTURED TIGER SHRIMP IN SEMI-INTENSIVE PONDS

Tiger shrimp diseases have occured in Indonesian shrimp ponds for more than two decades. In order to overcome this problem, five types of the RICA probiotic bacteria were tested in nine 250 m2 semi-intensive ponds of the Research and Development Institute for Coastal Aquaculture Marana Station. In the present study three different alternate use of the RICA probiotics were tested for tiger shrimp culture incompletely randomized design experiment. There were three treatments here namely: A) alternate use of probiotic bacteria RICA-1, RICA-2, RICA-3; B) alternate use of probiotic bacteria RICA-4, RICA-5, RICA-3, and C) control (without probiotic bacteria); each treatment were applied in three replications. The results showed that survival rate and production of tiger shrimp in treatment A (55.8% and 14.9 kg/pond) and B (52.7% and 16.7 kg/pond) were significantly better (P<0.05) than those of in control ponds (37.4% and 10.9 kg/pond). However, survival rate and production of tiger shrimp between treatment A and B were not significantly different (P>0.05). The average total bacteria population in the cultured water media of treatment A (4.32 x 104 cfu/mL) and B (5.18 x 104 cfu/mL) were relatively higher than control (1.46 x 104 cfu/mL). However the percentage ratio of Vibrio spp. and total bacteria population in the cultured water media of treatment A and B were relatively lower than control. The lower survival rate and production of tiger shrimp in control (C) compared to probiotic treatments (A and B) were not just affected by the increase of Vibrio spp. ratio, but also affected by the increase of total organic matter and nitrite concentrations in the control ponds that were relatively higher than treatment A and B

EFFECT OF DIFFERENT PROBIOTIC BACTERIA ON SURVIVAL RATE, GROWTH, AND PRODUCTION OF WHITELEG SHRIMP IN TRADITIONAL-PLUS TECHNOLOGY

Instead of culturing tiger shrimp that is frequently burdened by mass mortality, whiteleg shrimp (Litopenaeus vannamei) is then considered as an alternative commodity in Indonesian brackishwater ponds. To prevent the whiteleg shrimp from diseases, different probiotic bacteria were tested in completely randomized design experiment using nine 250 m2 experimental ponds stocked with 10 PLs of whiteleg shrimp fry/m2. Three treatments were applied, namely A) alternate use of probiotic bacteria RICA-1, RICA-2, RICA-3; B) alternate use of probiotic bacteria RICA-4, RICA-5, RICA-3, and C) control (without probiotic bacteria); each with three replications. After 11-week application, the results showed that the best survival rate of whiteleg shrimp was achieved by treatment B 98.83%) and the best production was achieved by treatment A (23.52 kg/250 m2). However, there were no significant differences (P>0.05) among the three treatments tested for the shrimp survival rate. The whiteleg shrimp production in treatment A and B were signicantly better (P<0.05) than that in treatment C (control). These high shrimp production in treatment A and B were mainly caused by the capability of the applied probiotics in controlling some water quality variables and Vibrio numbers

PERFORMANCE OF CULTURED White-leg Shrimp IN RICA Probiotic Application METHOD in PONDS AERATED WITH SUPERCHARGE BLOWER

Several ways have been done to encounter shrimp disease affecting cultured shrimp in Indonesian ponds in the last two decades. This research was aimed to find out the effect of different application of probiotic RICA4, RICA5, and RICA3 method on survival rate and production of white-leg shrimp (Litopenaeus vannamei) cultured in ponds aerated with supercharge blower. RICA probiotics are bacteria probiotics produced by the Research and Development Institute for Coastal Aquaculture, originally isolated from seaweed and sea sediment. This experiment was carried out in completely randomized design using nine 250-m2 experimental ponds stocked with 15 shrimp fries/m2. There were three treatments namely: A=alternate use of three probiotics RICA4, RICA5, and RICA3; B=combination use of three probiotics RICA4, RICA5, and RICA3; and C=control (without probiotic), each treatment with three replications and cultured with supercharge blower. Variables observed in this study were survival rate and production of the shrimp calculated at the end of experiment, total vibrio count (TBV) and total plate count of common bacteria (TPC) of the pond waters and sediments monitored every two weeks. The results showed that application of probiotic RICA4, RICA5, and RICA3 applied either in alteration or in combination significantly increased survival rate (P<0.05) but not on production (P>0.05) of the white-leg shrimp. TBV/TPC ratio in the control pond waters after 10-weeks culture (over than 10%) was relatively dangerous for the cultured white-leg shrimp. This shows that application of probiotic could prevent the growth of Vibrio spp in the cultured shrimp pond water

PENGARUH PERBEDAAN WAKTU APLIKASI PROBIOTIK TERHADAP KUALITAS AIR DAN SINTASAN PASCA LARVA UDANG WINDU (Penaeus monodon)

Penelitian ditujukan untuk mengetahui pengaruh waktu pemberian probiotik yang berbeda terhadap perubahan kualitas air dan sintasan udang windu dalam skala laboratorium. Penelitian dilaksanakan di Laboratorium Basah Balai Riset Perikanan Budidaya Air Payau (BRPBAP), Maros menggunakan 21 buah akuarium berukuran 40 cm x 30 cm x 27 cm yang diisi tanah tambak setebal 10 cm dan air laut salinitas 28 ppt sebanyak 15 L serta 30 ekor pascalarva udang windu. Probiotik yang digunakan adalah kombinasi BL542+BT951+MY1112 dengan perlakuan; (A) aplikasi probiotik pada awal sampai akhir penelitian; (B) aplikasi probiotik pada minggu ke-II sampai akhir penelitian; (C) aplikasi probiotik pada minggu ke-IV sampai akhir penelitian; (D) aplikasi probiotik pada minggu ke-VI sampai akhir penelitian; (E) aplikasi probiotik pada minggu ke-VIII sampai akhir penelitian; (F) aplikasi probiotik pada minggu ke X sampai akhir penelitian; (G) kontrol (tanpa probiotik), masing-masing diulang 3 kali. Penelitian berlangsung selama 12 minggu. Pengamatan parameter kualitas air dilakukan setiap 2 minggu yang meliputi; total bakteri, total Vibrio spp., BOT, NH3-N, NO2-N, NO3-N, PO4-P. Sedangkan pengamatan sintasan udang windu dilakukan pada akhir penelitian. Hasil penelitian menunjukkan bahwa aplikasi probiotik pada minggu ke-IV dapat menekan konsentrasi BOT dan NH3-N, menurunkan total Vibrio sp. sehingga berdampak kepada peningkatan sintasan udang wind

PATOGENISITAS BEBERAPA BAKTERI Vibrio YANG DIISOLASI DARI SEDIMEN TAMBAK TERHADAP UDANG WINDu, penaeus monodon

Penelitian bertujuan untuk mengetahui tingkat patogenisitas beberapa bakteri Vibrio yang diisolasi dari sedimen tambak terhadap udang windu , Penaeus monodon. penelitian dilakukan di Laboratorium Basah Balai Penelitian Perikanan Pantai yang meliputi beberapa tahapan kerja yaitu: (1) lsolasidan identifikasi bakteri vibrio dari sedimen tambak, (2) perbanyakan bakterivibiio hasil identifikasi menggunakan nutrient broth, (3) Infeksi buatan dengan bakteri V. alginotyticus, V. costicola, V. harueyi, atau V. mimicus dengan kepadatan 102, 104, dan 106 cfu/ml kedalam wadah pemeliharaan udang windu, dan (4) Pengamatan perkembangan populasi bakteri Vibrio oan mortalitas udang windu

PENGGUNAAN TIGA JENIS KERANG SEBAGAI BIOFILTER PADA PEMELIHARAAN UDANG WINDU (Penaeus monodon) DALAM SKALA LABORATORIUM

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan tiga jenis kekerangan sebagai biolilter media pemeliharaan udang windu terhadap laju pertumbuhan dan sintasan udang windu

PENYEBARAN DAN PREVALENSI WHITE SPOT SYNDROME VIRUS (WSSV) PADA BUDI DAYA UDANG WINDU (Penaeus monodon)

Penelitian ini bertujuan untuk mengetahui penyebaran dan tingkat prevalensi serangan WSSV pada budi daya udang windu. Pengumpulan sampel dan deteksi WSSV dengan teknik PCR dilakukan dari bulan April 2004 sampai November 2006. Sampel induk udang windu yang dikumpulkan berasal dari perairan Jawa Tengah, Sulawesi Selatan, Gorontalo, Kalimantan Timur, dan Timika. Sedangkan benur, tokolan, dan udang yang dibudidayakan dikumpulkan dari beberapa lokasi di Sulawesi Selatan. Sampel udang diambil bagian kaki jalan, kaki renang, tangkai mata, karapaks, insang, dan ekor. Benur yang berjumlah ± 30 ekor diekstrak menggunakan buffer lisis untuk mendapatkan DNA total. DNA WSSV diamplifikasi dengan teknik First dan Nested. PCR menggunakan kit amplifikasi spesifik WSSV (IQ 2000TM WSSV Detection and Prevention System). Visualisasi DNA WSSV dilakukan dengan gell documentation. Hasil penelitian menunjukkan, bahwa lebih dari 33% daerah sumber induk udang windu di Indonesia dan 90% daerah pertambakan yang ada di Sulawesi Selatan telah terinfeksi oleh WSSV. WSSV ditemukan pada induk, benur, tokolan, dan udang yang dibudidayakan di tambak. Dengan tingkat prevalensi serangan WSSV tertinggi pada udang windu yang dibudidayakan di tambak adalah 40,4% dan terendah pada benur 4,4%.The aims of this experiment was to know the distribution and prevalences of WSSV on tiger shrimp. Sample collection and WSSV detection conducted with PCR method was carried out during April 2004 to November 2006. Tiger shrimp broodstock samples were collected from Central Java, South Sulawesi, Gorontalo, Kalimantan, and Timika waters, and the other samples (tiger shrimp post larvae, juveniles, and cultured shrimp) were collected from several region in South Sulawesi. The pleopod, pereiopod, eye stalk, carapax, gill, tail, muscle of broodstock, juveniles, and cultured shrimp, and 30 pcs of postlarvae were extracted using lysis buffer to collect genomic DNA. WSSV DNA amplification was carried out using first and nested PCR technique by specific sequence amplification kit (IQ2000TM  Detection and Prevention system). The WSSV DNA was visualized by gell documentation system. The result showed that more than 33% of broodstock resources of Indonesia waters and 90% of shrimp culture area of South Sulawesi were contaminated by WSSV. WSSV was also infected tiger shrimp broodstock, postlarvae, juveniles, and tiger shrimp cultured with the highest prevalence (40.4%) was on tiger shrimp cultured and the lowest prevalence (5.4%) was on postlarvae

PENGARUH PENGGUNAAN KEKERANGAN SEBAGAI BIOFILTER TERHADAP KELIMPAHAN DAN KOMPOSISI JENIS BAKTERI PADA BUDIDAYA UDANG WINDU (Penaeus monodon) DENGAN SISTEM RESIRKULASI AIR

Penelitian ini bertujuan untuk melihat pengaruh penggunaan kekerangan terhadapkomposisi jenis dan kelimpahan bakteri pada pemeliharaan udang windu (Perweue monodon) dalam wadah dengan sistem resirkulasi air

KARAKTERISASI,ANALISIS GEN 16S-rRNA BAKTERT BL542 DAN EVALUASI EFEK BAKTERISIDANYA TERHADAP Vibrio harveyi PENYEBAB PENYAKIT PADA UDANG WINDU (penaeus monodon)

Penelitian bertujuan untuk mengetahui karakteristik, mengevaluasi efek bakterisida, dan menentukan posisi relatif isolat BL542 melalui analisis sekuen 16S-rRNA. Penelitian ini terdiri atas beberapa tahapan kerja yaitu: (1) karakterisasi fisiologi dan biokimia, (2) uji sensitivitas terhadap antibiotik, (3) uji daya hambat isolat BL542 terhadap V. Harveyi, (4) uji patogenisitas isolat BL542 terhadap larva udang, (5) uji tantang secara in vitro maupun secara in vivo isolat BL542 dengan V. Haeveyi, (6) analisis gen 16S-rRNA isolat BLS42

EFEKTIVITAS PEMBERIAN SEL UTUH (WHOLE CELL) BAKTERI VIBRIO TERHADAP PENINGKATAN KEKEBALAN TUBUH UDANG WINDU (Penaeus monodon FABR.) DARI SERANGAN WHITE SPOT SYNDROME VIRUS(WSSV)

Penelitian ini bertujuan untuk mengetahui efektivitas penggunaan lipopolisakarida (bakterin) dan konsentrasi bakterin paling tepat yang dapat memberikan pengaruh terbaik terhadap peningkatan kekebalan tubuh serta sintasan benur windu dari serangan WSSV. Lipopolisakarida yang digunakan dalam penelitian ini adalah hasil ekstraksi dari baktiri Vibrio harueyi. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan yaitu pemberian bakterin dengan konsentrasi 0 mg/L (A = kontrot), 20 glkg pakan (B), 30 g/kg pakin (cj, dan 40 g/ kg pakan (D). Ulangan dilakukan sebanyak 3 kali untuk tiap perlakuan aan setiap ulangan menggunakan 80 ekor benur windu